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Dietary alpha-ketoglutarate increases cold tolerance in Drosophila melanogaster and enhances protein pool and antioxidant defense in sex-specific manner

Bayliak, Maria M.; Lylyk, Maria P.; Shmihel, Halyna V.; Sorochynska, Oksana M.; Manyukh, Oksana V.; Pierzynowski, Stefan G. LU and Lushchak, Volodymyr I. (2016) In Journal of Thermal Biology 60. p.1-11
Abstract

Alpha-ketoglutarate (AKG) is an important intermediate in Krebs cycle which bridges the metabolism of amino acids and carbohydrates. Its effects as a dietary supplement on cold tolerance were studied in Drosophila melanogaster Canton S. Two-day-old adult flies fed at larval and adult stages with AKG at moderate concentrations (5-10 mM) recovered faster from chill coma (0 °C for 15 min or 3 h) than control ones. The beneficial effect of AKG on chill coma recovery was not found at its higher concentrations, which suggests hormetic like action of this keto acid. Time of 50% observed mortality after 2 h recovery from continuous cold exposure (-1 °C for 3-31 h) (LTi50) was higher for flies reared on 10 mM AKG compared with control... (More)

Alpha-ketoglutarate (AKG) is an important intermediate in Krebs cycle which bridges the metabolism of amino acids and carbohydrates. Its effects as a dietary supplement on cold tolerance were studied in Drosophila melanogaster Canton S. Two-day-old adult flies fed at larval and adult stages with AKG at moderate concentrations (5-10 mM) recovered faster from chill coma (0 °C for 15 min or 3 h) than control ones. The beneficial effect of AKG on chill coma recovery was not found at its higher concentrations, which suggests hormetic like action of this keto acid. Time of 50% observed mortality after 2 h recovery from continuous cold exposure (-1 °C for 3-31 h) (LTi50) was higher for flies reared on 10 mM AKG compared with control ones, showing that the diet with AKG enhanced insect cold tolerance. In parallel with enhancement of cold tolerance, dietary AKG improved fly locomotor activity. Metabolic effects of AKG differed partly in males and females. In males fed on AKG, there were no differences in total protein and free amino acid levels, but the total antioxidant capacity, catalase activity and low molecular mass thiol content were higher than in control animals. In females, dietary AKG promoted higher total antioxidant capacity and higher levels of proteins, total amino acids, proline and low molecular mass thiols. The levels of lipid peroxides were lower in both fly sexes reared on AKG as compared with control ones. We conclude that both enhancement of antioxidant system capacity and synthesis of amino acids can be important for AKG-promoted cold tolerance in D. melanogaster. The involvement of AKG in metabolic pathways of Drosophila males and females is discussed.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Amino acids, Chill coma, Proline, Stress recovery, Thiols
in
Journal of Thermal Biology
volume
60
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:84973299519
  • wos:000382349700001
ISSN
0306-4565
DOI
10.1016/j.jtherbio.2016.06.001
language
English
LU publication?
yes
id
9cda2dcd-173a-4ca8-8f66-b9e12e0eb5a9
date added to LUP
2016-12-15 10:42:50
date last changed
2017-09-18 11:31:22
@article{9cda2dcd-173a-4ca8-8f66-b9e12e0eb5a9,
  abstract     = {<p>Alpha-ketoglutarate (AKG) is an important intermediate in Krebs cycle which bridges the metabolism of amino acids and carbohydrates. Its effects as a dietary supplement on cold tolerance were studied in Drosophila melanogaster Canton S. Two-day-old adult flies fed at larval and adult stages with AKG at moderate concentrations (5-10 mM) recovered faster from chill coma (0 °C for 15 min or 3 h) than control ones. The beneficial effect of AKG on chill coma recovery was not found at its higher concentrations, which suggests hormetic like action of this keto acid. Time of 50% observed mortality after 2 h recovery from continuous cold exposure (-1 °C for 3-31 h) (LTi<sub>50</sub>) was higher for flies reared on 10 mM AKG compared with control ones, showing that the diet with AKG enhanced insect cold tolerance. In parallel with enhancement of cold tolerance, dietary AKG improved fly locomotor activity. Metabolic effects of AKG differed partly in males and females. In males fed on AKG, there were no differences in total protein and free amino acid levels, but the total antioxidant capacity, catalase activity and low molecular mass thiol content were higher than in control animals. In females, dietary AKG promoted higher total antioxidant capacity and higher levels of proteins, total amino acids, proline and low molecular mass thiols. The levels of lipid peroxides were lower in both fly sexes reared on AKG as compared with control ones. We conclude that both enhancement of antioxidant system capacity and synthesis of amino acids can be important for AKG-promoted cold tolerance in D. melanogaster. The involvement of AKG in metabolic pathways of Drosophila males and females is discussed.</p>},
  author       = {Bayliak, Maria M. and Lylyk, Maria P. and Shmihel, Halyna V. and Sorochynska, Oksana M. and Manyukh, Oksana V. and Pierzynowski, Stefan G. and Lushchak, Volodymyr I.},
  issn         = {0306-4565},
  keyword      = {Amino acids,Chill coma,Proline,Stress recovery,Thiols},
  language     = {eng},
  month        = {08},
  pages        = {1--11},
  publisher    = {Elsevier},
  series       = {Journal of Thermal Biology},
  title        = {Dietary alpha-ketoglutarate increases cold tolerance in Drosophila melanogaster and enhances protein pool and antioxidant defense in sex-specific manner},
  url          = {http://dx.doi.org/10.1016/j.jtherbio.2016.06.001},
  volume       = {60},
  year         = {2016},
}