Particulate matter constituents trigger the formation of extracellular amyloid β and Tau -containing plaques and neurite shortening in vitro
Sebastijanović, Aleksandar ; Azzurra Camassa, Laura Maria ; Malmborg, Vilhelm LU
; Kralj, Slavko
; Pagels, Joakim
LU
; Vogel, Ulla
; Zienolddiny-Narui, Shan
; Urbančič, Iztok
; Koklič, Tilen
and Štrancar, Janez
(2024)
In Nanotoxicology
18(4).
p.335-353
- Abstract
Air pollution is an environmental factor associated with an increased risk of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, characterized by decreased cognitive abilities and memory. The limited models of sporadic Alzheimer’s disease fail to replicate all pathological hallmarks of the disease, making it challenging to uncover potential environmental causes. Environmentally driven models of Alzheimer’s disease are thus timely and necessary. We used live-cell confocal fluorescent imaging combined with high-resolution stimulated emission depletion (STED) microscopy to follow the response of retinoic acid-differentiated human neuroblastoma SH-SY5Y cells to nanomaterial exposure. Here, we report that exposure of the cells... (More)
Air pollution is an environmental factor associated with an increased risk of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, characterized by decreased cognitive abilities and memory. The limited models of sporadic Alzheimer’s disease fail to replicate all pathological hallmarks of the disease, making it challenging to uncover potential environmental causes. Environmentally driven models of Alzheimer’s disease are thus timely and necessary. We used live-cell confocal fluorescent imaging combined with high-resolution stimulated emission depletion (STED) microscopy to follow the response of retinoic acid-differentiated human neuroblastoma SH-SY5Y cells to nanomaterial exposure. Here, we report that exposure of the cells to some particulate matter constituents reproduces a neurodegenerative phenotype, including extracellular amyloid beta-containing plaques and decreased neurite length. Consistent with the existing in vivo research, we observed detrimental effects, specifically a substantial reduction in neurite length and formation of amyloid beta plaques, after exposure to iron oxide and diesel exhaust particles. Conversely, after exposure to engineered cerium oxide nanoparticles, the lengths of neurites were maintained, and almost no extracellular amyloid beta plaques were formed. Although the exact mechanism behind this effect remains to be explained, the retinoic acid differentiated SH-SY5Y cell in vitro model could serve as an alternative, environmentally driven model of neurodegenerative diseases, including Alzheimer’s disease.
(Less)
- author
- Sebastijanović, Aleksandar
; Azzurra Camassa, Laura Maria
; Malmborg, Vilhelm
LU
; Kralj, Slavko
; Pagels, Joakim
LU
; Vogel, Ulla
; Zienolddiny-Narui, Shan
; Urbančič, Iztok
; Koklič, Tilen
and Štrancar, Janez
- organization
- publishing date
- 2024
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- air pollution, amyloid β (Aβ), CeO nanoparticles, diesel exhaust, iron oxide, microtubule-associated protein (Tau), Neurite shortening, neuronal degeneration, particulate matter constituents, TiO nanotubes
- in
- Nanotoxicology
- volume
- 18
- issue
- 4
- pages
- 19 pages
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:38907733
- scopus:85196626395
- ISSN
- 1743-5390
- DOI
- 10.1080/17435390.2024.2362367
- language
- English
- LU publication?
- yes
- id
- 9dfa2957-9c49-4485-872b-34c5ea4018bf
- date added to LUP
- 2024-09-02 11:21:34
- date last changed
- 2024-09-02 11:22:24
@article{9dfa2957-9c49-4485-872b-34c5ea4018bf,
abstract = {{<p>Air pollution is an environmental factor associated with an increased risk of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, characterized by decreased cognitive abilities and memory. The limited models of sporadic Alzheimer’s disease fail to replicate all pathological hallmarks of the disease, making it challenging to uncover potential environmental causes. Environmentally driven models of Alzheimer’s disease are thus timely and necessary. We used live-cell confocal fluorescent imaging combined with high-resolution stimulated emission depletion (STED) microscopy to follow the response of retinoic acid-differentiated human neuroblastoma SH-SY5Y cells to nanomaterial exposure. Here, we report that exposure of the cells to some particulate matter constituents reproduces a neurodegenerative phenotype, including extracellular amyloid beta-containing plaques and decreased neurite length. Consistent with the existing in vivo research, we observed detrimental effects, specifically a substantial reduction in neurite length and formation of amyloid beta plaques, after exposure to iron oxide and diesel exhaust particles. Conversely, after exposure to engineered cerium oxide nanoparticles, the lengths of neurites were maintained, and almost no extracellular amyloid beta plaques were formed. Although the exact mechanism behind this effect remains to be explained, the retinoic acid differentiated SH-SY5Y cell in vitro model could serve as an alternative, environmentally driven model of neurodegenerative diseases, including Alzheimer’s disease.</p>}},
author = {{Sebastijanović, Aleksandar and Azzurra Camassa, Laura Maria and Malmborg, Vilhelm and Kralj, Slavko and Pagels, Joakim and Vogel, Ulla and Zienolddiny-Narui, Shan and Urbančič, Iztok and Koklič, Tilen and Štrancar, Janez}},
issn = {{1743-5390}},
keywords = {{air pollution; amyloid β (Aβ); CeO nanoparticles; diesel exhaust; iron oxide; microtubule-associated protein (Tau); Neurite shortening; neuronal degeneration; particulate matter constituents; TiO nanotubes}},
language = {{eng}},
number = {{4}},
pages = {{335--353}},
publisher = {{Informa Healthcare}},
series = {{Nanotoxicology}},
title = {{Particulate matter constituents trigger the formation of extracellular amyloid β and Tau -containing plaques and neurite shortening in vitro}},
url = {{http://dx.doi.org/10.1080/17435390.2024.2362367}},
doi = {{10.1080/17435390.2024.2362367}},
volume = {{18}},
year = {{2024}},
}