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First Evidence of Acyl-Hydrolase/Lipase Activity From Human Probiotic Bacteria: Lactobacillus rhamnosus GG and Bifidobacterium longum NCC 2705

Manasian, Panagiotis ; Bustos, Atma-Sol LU ; Pålsson, Björn ; Håkansson, Andreas LU ; Peñarrieta, J. Mauricio ; Nilsson, Lars LU and Linares-Pastén, Javier A. LU (2020) In Frontiers in Microbiology
Abstract
Lactobacillus rhamnosus GG (ATCC 53103) and Bifidobacterium longum NCC 2705 are among the most studied probiotics. However, the first evidence of acyl hydrolase/lipase of two annotated proteins, one in each genome of these strains, is reported in this work. Signal peptide analysis has predicted that these proteins are exported to the extracellular medium. Both proteins were produced in Escherichia coli, purified and characterized. Molecular masses (without signal peptides) were 27 and 52.3 kDa for the proteins of L. rhamnosus and B. longum, respectively. Asymmetrical flow field-flow fractionation analysis has shown that both proteins are present as monomers in their native forms at pH 7. Both have shown enzymatic activity on pNP-laurate at... (More)
Lactobacillus rhamnosus GG (ATCC 53103) and Bifidobacterium longum NCC 2705 are among the most studied probiotics. However, the first evidence of acyl hydrolase/lipase of two annotated proteins, one in each genome of these strains, is reported in this work. Signal peptide analysis has predicted that these proteins are exported to the extracellular medium. Both proteins were produced in Escherichia coli, purified and characterized. Molecular masses (without signal peptides) were 27 and 52.3 kDa for the proteins of L. rhamnosus and B. longum, respectively. Asymmetrical flow field-flow fractionation analysis has shown that both proteins are present as monomers in their native forms at pH 7. Both have shown enzymatic activity on pNP-laurate at pH 7 and 37°C. The enzyme from L. rhamnosus was characterized deeper, showing preference on pNP-esters with short chain fatty acids. In addition, a computational model of the 3D structure has allowed the prediction of the catalytic amino acids. The enzymatic activities using synthetic substrates were very low for both enzymes. The investigation of natural substrates and biological functions of these enzymes is still open. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acyl-hydrolase/lipase, Lactobacillus rhamnosus GG, Biffidobacterium longum,
in
Frontiers in Microbiology
article number
1534
publisher
Frontiers
external identifiers
  • scopus:85089238249
  • pmid:32793131
ISSN
1664-302X
DOI
10.3389/fmicb.2020.01534
language
English
LU publication?
yes
id
a10385d0-c9b1-4c2b-bcbd-df6621d827cf
date added to LUP
2020-08-07 13:08:58
date last changed
2020-08-26 05:17:50
@article{a10385d0-c9b1-4c2b-bcbd-df6621d827cf,
  abstract     = {Lactobacillus rhamnosus GG (ATCC 53103) and Bifidobacterium longum NCC 2705 are among the most studied probiotics. However, the first evidence of acyl hydrolase/lipase of two annotated proteins, one in each genome of these strains, is reported in this work. Signal peptide analysis has predicted that these proteins are exported to the extracellular medium. Both proteins were produced in Escherichia coli, purified and characterized. Molecular masses (without signal peptides) were 27 and 52.3 kDa for the proteins of L. rhamnosus and B. longum, respectively. Asymmetrical flow field-flow fractionation analysis has shown that both proteins are present as monomers in their native forms at pH 7. Both have shown enzymatic activity on pNP-laurate at pH 7 and 37°C. The enzyme from L. rhamnosus was characterized deeper, showing preference on pNP-esters with short chain fatty acids. In addition, a computational model of the 3D structure has allowed the prediction of the catalytic amino acids. The enzymatic activities using synthetic substrates were very low for both enzymes. The investigation of natural substrates and biological functions of these enzymes is still open.},
  author       = {Manasian, Panagiotis and Bustos, Atma-Sol and Pålsson, Björn and Håkansson, Andreas and Peñarrieta, J. Mauricio and Nilsson, Lars and Linares-Pastén, Javier A.},
  issn         = {1664-302X},
  language     = {eng},
  publisher    = {Frontiers},
  series       = {Frontiers in Microbiology},
  title        = {First Evidence of Acyl-Hydrolase/Lipase Activity From Human Probiotic Bacteria: Lactobacillus rhamnosus GG and Bifidobacterium longum NCC 2705},
  url          = {http://dx.doi.org/10.3389/fmicb.2020.01534},
  doi          = {10.3389/fmicb.2020.01534},
  year         = {2020},
}