The Design of 3D-Printed Polylactic Acid–Bioglass Composite Scaffold : A Potential Implant Material for Bone Tissue Engineering
(2022) In Molecules 27(21).- Abstract
Bio-based and patient-specific three-dimensional (3D) scaffolds can present next generation strategies for bone tissue engineering (BTE) to treat critical bone size defects. In the present study, a composite filament of poly lactic acid (PLA) and 45S5 bioglass (BG) were used to 3D print scaffolds intended for bone tissue regeneration. The thermally induced phase separation (TIPS) technique was used to produce composite spheres that were extruded into a continuous filament to 3D print a variety of composite scaffolds. These scaffolds were analyzed for their macro- and microstructures, mechanical properties, in vitro cytotoxicity and in vivo biocompatibility. The results show that the BG particles were homogeneously distributed within the... (More)
Bio-based and patient-specific three-dimensional (3D) scaffolds can present next generation strategies for bone tissue engineering (BTE) to treat critical bone size defects. In the present study, a composite filament of poly lactic acid (PLA) and 45S5 bioglass (BG) were used to 3D print scaffolds intended for bone tissue regeneration. The thermally induced phase separation (TIPS) technique was used to produce composite spheres that were extruded into a continuous filament to 3D print a variety of composite scaffolds. These scaffolds were analyzed for their macro- and microstructures, mechanical properties, in vitro cytotoxicity and in vivo biocompatibility. The results show that the BG particles were homogeneously distributed within the PLA matrix and contributed to an 80% increase in the mechanical strength of the scaffolds. The in vitro cytotoxicity analysis of PLA-BG scaffolds using L929 mouse fibroblast cells confirmed their biocompatibility. During the in vivo studies, the population of the cells showed an elevated level of macrophages and active fibroblasts that are involved in collagen extracellular matrix synthesis. This study demonstrates successful processing of PLA-BG 3D-printed composite scaffolds and their potential as an implant material with a tunable pore structure and mechanical properties for regenerative bone tissue engineering.
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- author
- Sultan, Sahar ; Thomas, Nebu ; Varghese, Mekha ; Dalvi, Yogesh ; Joy, Shilpa ; Hall, Stephen LU and Mathew, Aji P.
- organization
- publishing date
- 2022-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 3D printing, bioglass, bone scaffold, polylactic acid
- in
- Molecules
- volume
- 27
- issue
- 21
- article number
- 7214
- publisher
- MDPI AG
- external identifiers
-
- pmid:36364053
- scopus:85141846156
- ISSN
- 1420-3049
- DOI
- 10.3390/molecules27217214
- language
- English
- LU publication?
- yes
- id
- a192f5ce-09fc-4aec-a10e-98b5f1e89afa
- date added to LUP
- 2023-01-03 14:46:21
- date last changed
- 2024-04-18 11:56:31
@article{a192f5ce-09fc-4aec-a10e-98b5f1e89afa,
abstract = {{<p>Bio-based and patient-specific three-dimensional (3D) scaffolds can present next generation strategies for bone tissue engineering (BTE) to treat critical bone size defects. In the present study, a composite filament of poly lactic acid (PLA) and 45S5 bioglass (BG) were used to 3D print scaffolds intended for bone tissue regeneration. The thermally induced phase separation (TIPS) technique was used to produce composite spheres that were extruded into a continuous filament to 3D print a variety of composite scaffolds. These scaffolds were analyzed for their macro- and microstructures, mechanical properties, in vitro cytotoxicity and in vivo biocompatibility. The results show that the BG particles were homogeneously distributed within the PLA matrix and contributed to an 80% increase in the mechanical strength of the scaffolds. The in vitro cytotoxicity analysis of PLA-BG scaffolds using L929 mouse fibroblast cells confirmed their biocompatibility. During the in vivo studies, the population of the cells showed an elevated level of macrophages and active fibroblasts that are involved in collagen extracellular matrix synthesis. This study demonstrates successful processing of PLA-BG 3D-printed composite scaffolds and their potential as an implant material with a tunable pore structure and mechanical properties for regenerative bone tissue engineering.</p>}},
author = {{Sultan, Sahar and Thomas, Nebu and Varghese, Mekha and Dalvi, Yogesh and Joy, Shilpa and Hall, Stephen and Mathew, Aji P.}},
issn = {{1420-3049}},
keywords = {{3D printing; bioglass; bone scaffold; polylactic acid}},
language = {{eng}},
number = {{21}},
publisher = {{MDPI AG}},
series = {{Molecules}},
title = {{The Design of 3D-Printed Polylactic Acid–Bioglass Composite Scaffold : A Potential Implant Material for Bone Tissue Engineering}},
url = {{http://dx.doi.org/10.3390/molecules27217214}},
doi = {{10.3390/molecules27217214}},
volume = {{27}},
year = {{2022}},
}