Ion currents of cardiomyocytes in different regions of the Göttingen minipig heart
(2017) In Journal of Pharmacological and Toxicological Methods 86. p.12-18- Abstract
Introduction The Göttingen minipig is a promising model for pharmacological safety assessment and for translational research in cardiology. We have examined the main ion currents in cardiomyocytes of the minipig heart. Methods Cardiac cells were isolated from different cardiac regions (endo-, mid- and epicardial left ventricle and right ventricle) from Göttingen minipigs and examined using the whole cell patch clamp technique combined with pharmacological interventions. Results The inward rectifier (IK1), the delayed rectifier (IK), with the rapid and slow components, (IKr, IKs) and the L-type Ca2 + channel (ICa,L) were identified in the different regions of the heart,... (More)
Introduction The Göttingen minipig is a promising model for pharmacological safety assessment and for translational research in cardiology. We have examined the main ion currents in cardiomyocytes of the minipig heart. Methods Cardiac cells were isolated from different cardiac regions (endo-, mid- and epicardial left ventricle and right ventricle) from Göttingen minipigs and examined using the whole cell patch clamp technique combined with pharmacological interventions. Results The inward rectifier (IK1), the delayed rectifier (IK), with the rapid and slow components, (IKr, IKs) and the L-type Ca2 + channel (ICa,L) were identified in the different regions of the heart, whereas the Ca2 +-independent transient outward current (Ito1) was observed in only a few cells. IK1 was similar in the cardiac regions with a slightly lower value in the epicardial cells. IKs was smaller in epi- and endo-cardial regions. Discussion The equivalents of the main human cardiac ion currents are present in the minipig cardiomyocytes with the exception of the Ca2 +-independent Ito1. The study provides further evidence that the minipig is a valid model for investigating cardiovascular pharmacology.
(Less)
- author
- Arlock, Per LU ; Mow, Tomas ; Sjöberg, Trygve LU ; Arner, Anders LU ; Steen, Stig LU and Laursen, Morten
- organization
- publishing date
- 2017-07-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cardiac electrophysiology, Delayed rectifier K channel, Göttingen minipig, Heart, hERG, Inward rectifier K channel, Ion channel, L-type Ca channel, Patch clamp
- in
- Journal of Pharmacological and Toxicological Methods
- volume
- 86
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85014413301
- pmid:28254506
- wos:000405993700002
- ISSN
- 1056-8719
- DOI
- 10.1016/j.vascn.2017.02.022
- language
- English
- LU publication?
- yes
- id
- a23c32de-8f7f-4d9c-8d54-03003e09f0fe
- date added to LUP
- 2017-03-14 08:45:46
- date last changed
- 2024-06-10 15:25:31
@article{a23c32de-8f7f-4d9c-8d54-03003e09f0fe,
abstract = {{<p>Introduction The Göttingen minipig is a promising model for pharmacological safety assessment and for translational research in cardiology. We have examined the main ion currents in cardiomyocytes of the minipig heart. Methods Cardiac cells were isolated from different cardiac regions (endo-, mid- and epicardial left ventricle and right ventricle) from Göttingen minipigs and examined using the whole cell patch clamp technique combined with pharmacological interventions. Results The inward rectifier (I<sub>K1</sub>), the delayed rectifier (I<sub>K</sub>), with the rapid and slow components, (I<sub>Kr</sub>, I<sub>Ks</sub>) and the L-type Ca<sup>2 +</sup> channel (I<sub>Ca,L</sub>) were identified in the different regions of the heart, whereas the Ca<sup>2 +</sup>-independent transient outward current (I<sub>to1</sub>) was observed in only a few cells. I<sub>K1</sub> was similar in the cardiac regions with a slightly lower value in the epicardial cells. I<sub>Ks</sub> was smaller in epi- and endo-cardial regions. Discussion The equivalents of the main human cardiac ion currents are present in the minipig cardiomyocytes with the exception of the Ca<sup>2 +</sup>-independent I<sub>to1</sub>. The study provides further evidence that the minipig is a valid model for investigating cardiovascular pharmacology.</p>}},
author = {{Arlock, Per and Mow, Tomas and Sjöberg, Trygve and Arner, Anders and Steen, Stig and Laursen, Morten}},
issn = {{1056-8719}},
keywords = {{Cardiac electrophysiology; Delayed rectifier K channel; Göttingen minipig; Heart; hERG; Inward rectifier K channel; Ion channel; L-type Ca channel; Patch clamp}},
language = {{eng}},
month = {{07}},
pages = {{12--18}},
publisher = {{Elsevier}},
series = {{Journal of Pharmacological and Toxicological Methods}},
title = {{Ion currents of cardiomyocytes in different regions of the Göttingen minipig heart}},
url = {{http://dx.doi.org/10.1016/j.vascn.2017.02.022}},
doi = {{10.1016/j.vascn.2017.02.022}},
volume = {{86}},
year = {{2017}},
}