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Ion currents of cardiomyocytes in different regions of the Göttingen minipig heart

Arlock, Per LU ; Mow, Tomas; Sjöberg, Trygve LU ; Arner, Anders LU ; Steen, Stig LU and Laursen, Morten (2017) In Journal of Pharmacological and Toxicological Methods 86. p.12-18
Abstract

Introduction The Göttingen minipig is a promising model for pharmacological safety assessment and for translational research in cardiology. We have examined the main ion currents in cardiomyocytes of the minipig heart. Methods Cardiac cells were isolated from different cardiac regions (endo-, mid- and epicardial left ventricle and right ventricle) from Göttingen minipigs and examined using the whole cell patch clamp technique combined with pharmacological interventions. Results The inward rectifier (IK1), the delayed rectifier (IK), with the rapid and slow components, (IKr, IKs) and the L-type Ca2 + channel (ICa,L) were identified in the different regions of the heart,... (More)

Introduction The Göttingen minipig is a promising model for pharmacological safety assessment and for translational research in cardiology. We have examined the main ion currents in cardiomyocytes of the minipig heart. Methods Cardiac cells were isolated from different cardiac regions (endo-, mid- and epicardial left ventricle and right ventricle) from Göttingen minipigs and examined using the whole cell patch clamp technique combined with pharmacological interventions. Results The inward rectifier (IK1), the delayed rectifier (IK), with the rapid and slow components, (IKr, IKs) and the L-type Ca2 + channel (ICa,L) were identified in the different regions of the heart, whereas the Ca2 +-independent transient outward current (Ito1) was observed in only a few cells. IK1 was similar in the cardiac regions with a slightly lower value in the epicardial cells. IKs was smaller in epi- and endo-cardial regions. Discussion The equivalents of the main human cardiac ion currents are present in the minipig cardiomyocytes with the exception of the Ca2 +-independent Ito1. The study provides further evidence that the minipig is a valid model for investigating cardiovascular pharmacology.

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Contribution to journal
publication status
published
subject
keywords
Cardiac electrophysiology, Delayed rectifier K channel, Göttingen minipig, Heart, hERG, Inward rectifier K channel, Ion channel, L-type Ca channel, Patch clamp
in
Journal of Pharmacological and Toxicological Methods
volume
86
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:85014413301
  • wos:000405993700002
ISSN
1056-8719
DOI
10.1016/j.vascn.2017.02.022
language
English
LU publication?
yes
id
a23c32de-8f7f-4d9c-8d54-03003e09f0fe
date added to LUP
2017-03-14 08:45:46
date last changed
2018-01-07 11:55:08
@article{a23c32de-8f7f-4d9c-8d54-03003e09f0fe,
  abstract     = {<p>Introduction The Göttingen minipig is a promising model for pharmacological safety assessment and for translational research in cardiology. We have examined the main ion currents in cardiomyocytes of the minipig heart. Methods Cardiac cells were isolated from different cardiac regions (endo-, mid- and epicardial left ventricle and right ventricle) from Göttingen minipigs and examined using the whole cell patch clamp technique combined with pharmacological interventions. Results The inward rectifier (I<sub>K1</sub>), the delayed rectifier (I<sub>K</sub>), with the rapid and slow components, (I<sub>Kr</sub>, I<sub>Ks</sub>) and the L-type Ca<sup>2 +</sup> channel (I<sub>Ca,L</sub>) were identified in the different regions of the heart, whereas the Ca<sup>2 +</sup>-independent transient outward current (I<sub>to1</sub>) was observed in only a few cells. I<sub>K1</sub> was similar in the cardiac regions with a slightly lower value in the epicardial cells. I<sub>Ks</sub> was smaller in epi- and endo-cardial regions. Discussion The equivalents of the main human cardiac ion currents are present in the minipig cardiomyocytes with the exception of the Ca<sup>2 +</sup>-independent I<sub>to1</sub>. The study provides further evidence that the minipig is a valid model for investigating cardiovascular pharmacology.</p>},
  author       = {Arlock, Per and Mow, Tomas and Sjöberg, Trygve and Arner, Anders and Steen, Stig and Laursen, Morten},
  issn         = {1056-8719},
  keyword      = {Cardiac electrophysiology,Delayed rectifier K channel,Göttingen minipig,Heart,hERG,Inward rectifier K channel,Ion channel,L-type Ca channel,Patch clamp},
  language     = {eng},
  month        = {07},
  pages        = {12--18},
  publisher    = {Elsevier},
  series       = {Journal of Pharmacological and Toxicological Methods},
  title        = {Ion currents of cardiomyocytes in different regions of the Göttingen minipig heart},
  url          = {http://dx.doi.org/10.1016/j.vascn.2017.02.022},
  volume       = {86},
  year         = {2017},
}