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A 5-hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance

Petersson, A; Almeida, Joao LU ; Modig, Tobias LU ; Karhumaa, Kaisa LU ; Hahn-Hägerdal, Bärbel LU ; Gorwa-Grauslund, Marie-Francoise LU and Lidén, Gunnar LU (2006) In Yeast 23(6). p.455-464
Abstract
The fermentation of lignocellulose hydrolysates by Saccharomyces cerevisiae for fuel ethanol production is inhibited by 5-hydroxymethyl furfural (HMF), a furan derivative which is formed during the hydrolysis of lignocellulosic materials. The inhibition can be avoided if the yeast strain used in the fermentation has the ability to reduce HMF to 5-hydroxymethylfurfuryl alcohol. To enable the identification of enzyme(s) responsible for HMF conversion in S. cerevisiae, microarray analyses of two strains with different abilities to convert HMF were performed. Based on the expression data, a subset of 15 reductase genes was chosen to be further examined using an overexpression strain collection. Three candidate genes were cloned from two... (More)
The fermentation of lignocellulose hydrolysates by Saccharomyces cerevisiae for fuel ethanol production is inhibited by 5-hydroxymethyl furfural (HMF), a furan derivative which is formed during the hydrolysis of lignocellulosic materials. The inhibition can be avoided if the yeast strain used in the fermentation has the ability to reduce HMF to 5-hydroxymethylfurfuryl alcohol. To enable the identification of enzyme(s) responsible for HMF conversion in S. cerevisiae, microarray analyses of two strains with different abilities to convert HMF were performed. Based on the expression data, a subset of 15 reductase genes was chosen to be further examined using an overexpression strain collection. Three candidate genes were cloned from two different strains, TMB3000 and the laboratory strain CEN.PK 113-5D, and overexpressed using a strong promoter in the strain CEN.PK 113-5D. Strains overexpressing ADH6 had increased HMF conversion activity in cell-free crude extracts with both NADPH and NADH as co-factors. In vitro activities were recorded of 8 mU/mg with NADH as co-factor and as high as 1200 mU/mg for the NADPH-coupled reduction. Yeast strains overexpressing ADH6 also had a substantially higher in vivo conversion rate of HMF in both aerobic and anaerobic cultures, showing that the overexpression indeed conveyed the desired increased reduction capacity. Copyright (c) 2006 John Wiley & Sons, Ltd. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hydrolysates, lignocellulose, genome-wide analysis, 5-hydroxymethyl furfural, ADH6, Saccharomyces cerevisiae
in
Yeast
volume
23
issue
6
pages
455 - 464
publisher
John Wiley & Sons
external identifiers
  • pmid:16652391
  • wos:000237859900003
  • scopus:33744474816
ISSN
1097-0061
DOI
10.1002/yea.1370
language
English
LU publication?
yes
id
a284b2f8-bd3b-4041-88b9-147a56f291fc (old id 407513)
date added to LUP
2007-10-14 13:08:40
date last changed
2019-03-19 02:30:40
@article{a284b2f8-bd3b-4041-88b9-147a56f291fc,
  abstract     = {The fermentation of lignocellulose hydrolysates by Saccharomyces cerevisiae for fuel ethanol production is inhibited by 5-hydroxymethyl furfural (HMF), a furan derivative which is formed during the hydrolysis of lignocellulosic materials. The inhibition can be avoided if the yeast strain used in the fermentation has the ability to reduce HMF to 5-hydroxymethylfurfuryl alcohol. To enable the identification of enzyme(s) responsible for HMF conversion in S. cerevisiae, microarray analyses of two strains with different abilities to convert HMF were performed. Based on the expression data, a subset of 15 reductase genes was chosen to be further examined using an overexpression strain collection. Three candidate genes were cloned from two different strains, TMB3000 and the laboratory strain CEN.PK 113-5D, and overexpressed using a strong promoter in the strain CEN.PK 113-5D. Strains overexpressing ADH6 had increased HMF conversion activity in cell-free crude extracts with both NADPH and NADH as co-factors. In vitro activities were recorded of 8 mU/mg with NADH as co-factor and as high as 1200 mU/mg for the NADPH-coupled reduction. Yeast strains overexpressing ADH6 also had a substantially higher in vivo conversion rate of HMF in both aerobic and anaerobic cultures, showing that the overexpression indeed conveyed the desired increased reduction capacity. Copyright (c) 2006 John Wiley & Sons, Ltd.},
  author       = {Petersson, A and Almeida, Joao and Modig, Tobias and Karhumaa, Kaisa and Hahn-Hägerdal, Bärbel and Gorwa-Grauslund, Marie-Francoise and Lidén, Gunnar},
  issn         = {1097-0061},
  keyword      = {hydrolysates,lignocellulose,genome-wide analysis,5-hydroxymethyl furfural,ADH6,Saccharomyces cerevisiae},
  language     = {eng},
  number       = {6},
  pages        = {455--464},
  publisher    = {John Wiley & Sons},
  series       = {Yeast},
  title        = {A 5-hydroxymethyl furfural reducing enzyme encoded by the Saccharomyces cerevisiae ADH6 gene conveys HMF tolerance},
  url          = {http://dx.doi.org/10.1002/yea.1370},
  volume       = {23},
  year         = {2006},
}