A Mixture of Endocrine Disrupting Chemicals Associated with Lower Birth Weight in Children Induces Adipogenesis and DNA Methylation Changes in Human Mesenchymal Stem Cells
(2022) In International Journal of Molecular Sciences 23(4).- Abstract
Endocrine disrupting chemicals (EDCs) are man-made compounds that alter functions of the endocrine system. Environmental mixtures of EDCs might have adverse effects on human health, even though their individual concentrations are below regulatory levels of concerns. However, studies identifying and experimentally testing adverse effects of real-life mixtures are scarce. In this study, we aimed at evaluating an epidemiologically identified EDC mixture in an experimental setting to delineate its cellular and epigenetic effects. The mixture was established using data from the Swedish Environmental Longitudinal Mother and child Asthma and allergy (SELMA) study where it was associated with lower birth weight, an early marker for prenatal... (More)
Endocrine disrupting chemicals (EDCs) are man-made compounds that alter functions of the endocrine system. Environmental mixtures of EDCs might have adverse effects on human health, even though their individual concentrations are below regulatory levels of concerns. However, studies identifying and experimentally testing adverse effects of real-life mixtures are scarce. In this study, we aimed at evaluating an epidemiologically identified EDC mixture in an experimental setting to delineate its cellular and epigenetic effects. The mixture was established using data from the Swedish Environmental Longitudinal Mother and child Asthma and allergy (SELMA) study where it was associated with lower birth weight, an early marker for prenatal metabolic programming. This mixture was then tested for its ability to change metabolic programming of human mesenchymal stem cells. In these cells, we assessed if the mixture induced adipogenesis and genome-wide DNA methylation changes. The mixture increased lipid droplet accumulation already at concentrations corresponding to levels measured in the pregnant women of the SELMA study. Furthermore, we identified differentially methylated regions in genes important for adipogenesis and thermogenesis. This study shows that a mixture reflecting human real-life exposure can induce molecular and cellular changes during development that could underlie adverse outcomes.
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- author
- Lizunkova, Polina ; Engdahl, Elin ; Borbély, Gábor ; Gennings, Chris ; Lindh, Christian LU ; Bornehag, Carl Gustaf LU and Rüegg, Joëlle
- organization
- publishing date
- 2022-02
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adipogenesis, Chemical mixtures, DNA methylation, EDC, Endocrine disrupting chemicals, Low birth weight, Mesenchymal stem cells
- in
- International Journal of Molecular Sciences
- volume
- 23
- issue
- 4
- article number
- 2320
- publisher
- MDPI AG
- external identifiers
-
- pmid:35216435
- scopus:85124884554
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms23042320
- language
- English
- LU publication?
- yes
- id
- a3055cf4-2e5c-47f9-8609-464752d9aa78
- date added to LUP
- 2022-04-13 10:39:53
- date last changed
- 2024-08-13 20:38:08
@article{a3055cf4-2e5c-47f9-8609-464752d9aa78, abstract = {{<p>Endocrine disrupting chemicals (EDCs) are man-made compounds that alter functions of the endocrine system. Environmental mixtures of EDCs might have adverse effects on human health, even though their individual concentrations are below regulatory levels of concerns. However, studies identifying and experimentally testing adverse effects of real-life mixtures are scarce. In this study, we aimed at evaluating an epidemiologically identified EDC mixture in an experimental setting to delineate its cellular and epigenetic effects. The mixture was established using data from the Swedish Environmental Longitudinal Mother and child Asthma and allergy (SELMA) study where it was associated with lower birth weight, an early marker for prenatal metabolic programming. This mixture was then tested for its ability to change metabolic programming of human mesenchymal stem cells. In these cells, we assessed if the mixture induced adipogenesis and genome-wide DNA methylation changes. The mixture increased lipid droplet accumulation already at concentrations corresponding to levels measured in the pregnant women of the SELMA study. Furthermore, we identified differentially methylated regions in genes important for adipogenesis and thermogenesis. This study shows that a mixture reflecting human real-life exposure can induce molecular and cellular changes during development that could underlie adverse outcomes.</p>}}, author = {{Lizunkova, Polina and Engdahl, Elin and Borbély, Gábor and Gennings, Chris and Lindh, Christian and Bornehag, Carl Gustaf and Rüegg, Joëlle}}, issn = {{1661-6596}}, keywords = {{Adipogenesis; Chemical mixtures; DNA methylation; EDC; Endocrine disrupting chemicals; Low birth weight; Mesenchymal stem cells}}, language = {{eng}}, number = {{4}}, publisher = {{MDPI AG}}, series = {{International Journal of Molecular Sciences}}, title = {{A Mixture of Endocrine Disrupting Chemicals Associated with Lower Birth Weight in Children Induces Adipogenesis and DNA Methylation Changes in Human Mesenchymal Stem Cells}}, url = {{http://dx.doi.org/10.3390/ijms23042320}}, doi = {{10.3390/ijms23042320}}, volume = {{23}}, year = {{2022}}, }