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Characterization of olfactory sensory neurons in the red clover seed weevil, Protapion trifolii (Coleoptera : Brentidae) and comparison to the closely related species P. fulvipes

Carrasco, David LU ; Nyabuga, Franklin N. LU ; Anderbrant, Olle LU ; Svensson, Glenn P. LU ; Birgersson, Göran LU ; Lankinen, Åsa LU ; Larsson, Mattias C. LU and Andersson, Martin N. LU (2019) In Journal of Insect Physiology 119.
Abstract

Protapion trifolii and P. fulvipes (Coleoptera: Brentidae) are major pests in European clover seed production. Previous studies have reported a high host plant fidelity of these weevils for red and white clover species, respectively, driven by host plant olfactory cues. Given the specific host preferences observed in these weevils, we aimed to elucidate to which extent such selectivity is reflected in their peripheral olfactory systems. Using an electrophysiological approach, we performed the first functional characterisation of olfactory sensory neurons (OSNs) in P. trifolii to a panel of volatile compounds emitted by red clover plants, and compared the results with the reported OSN types of P. fulvipes. Nineteen OSN classes were... (More)

Protapion trifolii and P. fulvipes (Coleoptera: Brentidae) are major pests in European clover seed production. Previous studies have reported a high host plant fidelity of these weevils for red and white clover species, respectively, driven by host plant olfactory cues. Given the specific host preferences observed in these weevils, we aimed to elucidate to which extent such selectivity is reflected in their peripheral olfactory systems. Using an electrophysiological approach, we performed the first functional characterisation of olfactory sensory neurons (OSNs) in P. trifolii to a panel of volatile compounds emitted by red clover plants, and compared the results with the reported OSN types of P. fulvipes. Nineteen OSN classes were characterized in P. trifolii, with the majority of these neurons responding strongly to common volatiles released by the host plant. Based on response profiles, eight of these OSN classes have clear matches to OSN classes in P. fulvipes. The OSN colocalisation patterns and antennal frequency of these classes are similar in the two species. Additionally, the responses of these OSNs are generally highly conserved in the two species, with clear response shifts only revealed for two of the OSN classes. These response shifts in combination with additional response dissimilarities for compounds that vary in abundance between red and white clover plants may underlie the species-specific host preferences. Further behavioural and field experiments should focus on these differentially detected compounds to elucidate their potential role in host selection and use in semiochemical-based control of these pests.

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author
; ; ; ; ; ; and
organization
publishing date
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Contribution to journal
publication status
published
subject
keywords
Clover odour, Host plant discrimination, Odor coding, Olfactory sensory neuron, Protapion, Single sensillum recordings
in
Journal of Insect Physiology
volume
119
article number
103948
publisher
Elsevier Ltd
external identifiers
  • pmid:31539550
  • scopus:85074248765
ISSN
0022-1910
DOI
10.1016/j.jinsphys.2019.103948
language
English
LU publication?
yes
id
a3ebeada-a884-4efb-a733-81fa422365d0
date added to LUP
2019-11-13 16:50:13
date last changed
2021-06-23 02:58:21
@article{a3ebeada-a884-4efb-a733-81fa422365d0,
  abstract     = {<p>Protapion trifolii and P. fulvipes (Coleoptera: Brentidae) are major pests in European clover seed production. Previous studies have reported a high host plant fidelity of these weevils for red and white clover species, respectively, driven by host plant olfactory cues. Given the specific host preferences observed in these weevils, we aimed to elucidate to which extent such selectivity is reflected in their peripheral olfactory systems. Using an electrophysiological approach, we performed the first functional characterisation of olfactory sensory neurons (OSNs) in P. trifolii to a panel of volatile compounds emitted by red clover plants, and compared the results with the reported OSN types of P. fulvipes. Nineteen OSN classes were characterized in P. trifolii, with the majority of these neurons responding strongly to common volatiles released by the host plant. Based on response profiles, eight of these OSN classes have clear matches to OSN classes in P. fulvipes. The OSN colocalisation patterns and antennal frequency of these classes are similar in the two species. Additionally, the responses of these OSNs are generally highly conserved in the two species, with clear response shifts only revealed for two of the OSN classes. These response shifts in combination with additional response dissimilarities for compounds that vary in abundance between red and white clover plants may underlie the species-specific host preferences. Further behavioural and field experiments should focus on these differentially detected compounds to elucidate their potential role in host selection and use in semiochemical-based control of these pests.</p>},
  author       = {Carrasco, David and Nyabuga, Franklin N. and Anderbrant, Olle and Svensson, Glenn P. and Birgersson, Göran and Lankinen, Åsa and Larsson, Mattias C. and Andersson, Martin N.},
  issn         = {0022-1910},
  language     = {eng},
  publisher    = {Elsevier Ltd},
  series       = {Journal of Insect Physiology},
  title        = {Characterization of olfactory sensory neurons in the red clover seed weevil, Protapion trifolii (Coleoptera : Brentidae) and comparison to the closely related species P. fulvipes},
  url          = {http://dx.doi.org/10.1016/j.jinsphys.2019.103948},
  doi          = {10.1016/j.jinsphys.2019.103948},
  volume       = {119},
  year         = {2019},
}