Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Two photoacoustic spectral coloring compensation techniques adapted to the context of human in-vivo oxygenation measurements

Khodaverdi, Azin LU ; Jayet, Baptiste ; Erlöv, Tobias LU ; Albinsson, John LU orcid ; Merdasa, Aboma LU orcid ; Gustafsson, Nils LU ; Sheikh, Rafi LU orcid ; Malmsjö, Malin LU orcid ; Andersson-Engels, Stefan LU and Cinthio, Magnus LU orcid (2025) In Biomedical Optics Express 16(6). p.2217-2231
Abstract

Photoacoustic imaging can potentially map oxygen saturation (sO2) non-invasively. However, in-vivo human application is challenging due to spectral coloring, which causes a wavelength-dependent fluence attenuation and uncertainty in the estimation of chromophore concentrations deep in tissue. This study compares the performances of two previously proposed methods for spectral coloring compensation on in-vivo human data. Both methods have been modified and adapted to this context. The first modified method was evaluated using a tissue-mimicking phantom, showing restoration of the original spectrum of the target and decreasing the relative mean square error from 65% to 1.2% for the highest concentration. Spatial maps of... (More)

Photoacoustic imaging can potentially map oxygen saturation (sO2) non-invasively. However, in-vivo human application is challenging due to spectral coloring, which causes a wavelength-dependent fluence attenuation and uncertainty in the estimation of chromophore concentrations deep in tissue. This study compares the performances of two previously proposed methods for spectral coloring compensation on in-vivo human data. Both methods have been modified and adapted to this context. The first modified method was evaluated using a tissue-mimicking phantom, showing restoration of the original spectrum of the target and decreasing the relative mean square error from 65% to 1.2% for the highest concentration. Spatial maps of sO2 were estimated from in-vivo human finger measurements using both methods and compared with linear unmixing. Both methods reconstructed comparable values of sO2 and reduced depth-dependent changes in sO2, typically seen with linear unmixing, resulting in a gradient of saturation closer to zero as expected physiologically.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomedical Optics Express
volume
16
issue
6
pages
2217 - 2231
publisher
Optical Society of America
external identifiers
  • scopus:105005006855
ISSN
2156-7085
DOI
10.1364/BOE.555305
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2025 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
id
a4dbea19-356e-4e28-ac28-fc9f24b59bde
date added to LUP
2025-06-09 08:53:25
date last changed
2025-06-09 09:01:41
@article{a4dbea19-356e-4e28-ac28-fc9f24b59bde,
  abstract     = {{<p>Photoacoustic imaging can potentially map oxygen saturation (sO<sub>2</sub>) non-invasively. However, in-vivo human application is challenging due to spectral coloring, which causes a wavelength-dependent fluence attenuation and uncertainty in the estimation of chromophore concentrations deep in tissue. This study compares the performances of two previously proposed methods for spectral coloring compensation on in-vivo human data. Both methods have been modified and adapted to this context. The first modified method was evaluated using a tissue-mimicking phantom, showing restoration of the original spectrum of the target and decreasing the relative mean square error from 65% to 1.2% for the highest concentration. Spatial maps of sO<sub>2 </sub>were estimated from in-vivo human finger measurements using both methods and compared with linear unmixing. Both methods reconstructed comparable values of sO<sub>2</sub> and reduced depth-dependent changes in sO<sub>2</sub>, typically seen with linear unmixing, resulting in a gradient of saturation closer to zero as expected physiologically.</p>}},
  author       = {{Khodaverdi, Azin and Jayet, Baptiste and Erlöv, Tobias and Albinsson, John and Merdasa, Aboma and Gustafsson, Nils and Sheikh, Rafi and Malmsjö, Malin and Andersson-Engels, Stefan and Cinthio, Magnus}},
  issn         = {{2156-7085}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{6}},
  pages        = {{2217--2231}},
  publisher    = {{Optical Society of America}},
  series       = {{Biomedical Optics Express}},
  title        = {{Two photoacoustic spectral coloring compensation techniques adapted to the context of human in-vivo oxygenation measurements}},
  url          = {{http://dx.doi.org/10.1364/BOE.555305}},
  doi          = {{10.1364/BOE.555305}},
  volume       = {{16}},
  year         = {{2025}},
}