Acoustic streaming on antibody-functionalized screen-printed electrode enhances detection sensitivity and total assay duration for voltammetric immunosensing of newcastle disease virus
(2025) In Bioelectrochemistry 166.- Abstract
Conventional diagnostic methods often involve long incubation times due to limited fluid mixing in confined spaces, despite offering high sensitivity. Therefore, acoustic streaming was employed to enhance microscale advection, thereby improving biomolecular interactions and reducing assay duration. The micromixing capability was demonstrated by dispersing methylene blue (MB) in deionized water and glycerol solutions, where homogenization time decreased by approximately 80 % in water and 84–88 % in glycerol under acoustic actuation. Biomolecule adsorption was modeled using MB adsorbed onto cellulose acetate–graphene oxide (CA-GO) beads, showing improved adsorption and a reduced time to saturation from 16 to 8 min. Maximum adsorption... (More)
Conventional diagnostic methods often involve long incubation times due to limited fluid mixing in confined spaces, despite offering high sensitivity. Therefore, acoustic streaming was employed to enhance microscale advection, thereby improving biomolecular interactions and reducing assay duration. The micromixing capability was demonstrated by dispersing methylene blue (MB) in deionized water and glycerol solutions, where homogenization time decreased by approximately 80 % in water and 84–88 % in glycerol under acoustic actuation. Biomolecule adsorption was modeled using MB adsorbed onto cellulose acetate–graphene oxide (CA-GO) beads, showing improved adsorption and a reduced time to saturation from 16 to 8 min. Maximum adsorption occurred at 2 MHz frequency and 20 V amplitude. By using these optimized parameters, voltammetric immunosensing of Newcastle disease virus (NDV) was performed on PEG-alkanethiol-modified screen-printed gold electrodes (SPGE). The system incorporating acoustic streaming was compared against one without it. Results demonstrated a comparable limit of detection (1.46 HA μL−1 at 3σ m−1) achieved at shorter assay duration (8 min). These findings underscore the potential of acoustic streaming in electrochemical immunosensors to accelerate diagnostic assays without compromising sensitivity or specificity, particularly for applications utilizing screen-printed electrodes.
(Less)
- author
- Abd Muain, Mohamad Farid ; Amir Hamzah, Amir Syahir ; Chia, Suet Lin ; Yusoff, Khatijah ; Lim, Hong Ngee ; Ikeno, Shinya ; Laurell, Thomas LU and Ahmad Tajudin, Asilah LU
- organization
-
- LTH Profile Area: Photon Science and Technology
- LU Profile Area: Light and Materials
- LTH Profile Area: Nanoscience and Semiconductor Technology
- LTH Profile Area: Engineering Health
- LUCC: Lund University Cancer Centre
- NanoLund: Centre for Nanoscience
- Division for Biomedical Engineering
- MultiPark: Multidisciplinary research focused on Parkinson's disease
- publishing date
- 2025-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acoustic streaming, Electrochemical immunosensor, Micromixing, Piezoelectric
- in
- Bioelectrochemistry
- volume
- 166
- article number
- 109043
- publisher
- Elsevier
- external identifiers
-
- scopus:105010675368
- pmid:40674883
- ISSN
- 1567-5394
- DOI
- 10.1016/j.bioelechem.2025.109043
- language
- English
- LU publication?
- yes
- id
- a4e0ba08-5fff-466b-8c58-97dcb7160b16
- date added to LUP
- 2025-10-28 11:00:53
- date last changed
- 2025-11-25 13:16:03
@article{a4e0ba08-5fff-466b-8c58-97dcb7160b16,
abstract = {{<p>Conventional diagnostic methods often involve long incubation times due to limited fluid mixing in confined spaces, despite offering high sensitivity. Therefore, acoustic streaming was employed to enhance microscale advection, thereby improving biomolecular interactions and reducing assay duration. The micromixing capability was demonstrated by dispersing methylene blue (MB) in deionized water and glycerol solutions, where homogenization time decreased by approximately 80 % in water and 84–88 % in glycerol under acoustic actuation. Biomolecule adsorption was modeled using MB adsorbed onto cellulose acetate–graphene oxide (CA-GO) beads, showing improved adsorption and a reduced time to saturation from 16 to 8 min. Maximum adsorption occurred at 2 MHz frequency and 20 V amplitude. By using these optimized parameters, voltammetric immunosensing of Newcastle disease virus (NDV) was performed on PEG-alkanethiol-modified screen-printed gold electrodes (SPGE). The system incorporating acoustic streaming was compared against one without it. Results demonstrated a comparable limit of detection (1.46 HA μL<sup>−1</sup> at 3σ m<sup>−1</sup>) achieved at shorter assay duration (8 min). These findings underscore the potential of acoustic streaming in electrochemical immunosensors to accelerate diagnostic assays without compromising sensitivity or specificity, particularly for applications utilizing screen-printed electrodes.</p>}},
author = {{Abd Muain, Mohamad Farid and Amir Hamzah, Amir Syahir and Chia, Suet Lin and Yusoff, Khatijah and Lim, Hong Ngee and Ikeno, Shinya and Laurell, Thomas and Ahmad Tajudin, Asilah}},
issn = {{1567-5394}},
keywords = {{Acoustic streaming; Electrochemical immunosensor; Micromixing; Piezoelectric}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Bioelectrochemistry}},
title = {{Acoustic streaming on antibody-functionalized screen-printed electrode enhances detection sensitivity and total assay duration for voltammetric immunosensing of newcastle disease virus}},
url = {{http://dx.doi.org/10.1016/j.bioelechem.2025.109043}},
doi = {{10.1016/j.bioelechem.2025.109043}},
volume = {{166}},
year = {{2025}},
}