Advanced

Time and concentration dependence of Fenton-induced oxidation of dG

Grey, Carl LU and Adlercreutz, Patrick LU (2006) In Nucleosides, Nucleotides & Nucleic Acids 25(3). p.259-278
Abstract
The influence of incubation time and Fenton reagent concentrations was investigated on the oxidation of 2'-deoxyguanosine. The compounds identified and quantified, through use of an LC-MS/MS system, were 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'- cyclo-2'-deoxyguanosine (8,5'cyclodG) and the secondary oxidation products guanidinohydantoin and dehydro-guanidinohydantoin. 8-oxodG and 8,5'cyclodG formed very quickly, reaching a maximum rapidly, but with 8-oxodC a rapid decline occurred thereafter due to its further oxidation into the secondary products, which formed more slowly. Due to the better stability, 8,5'cyclodG correlated better with the general level of oxidation than 8-oxodG. The results emphasize the advantages of... (More)
The influence of incubation time and Fenton reagent concentrations was investigated on the oxidation of 2'-deoxyguanosine. The compounds identified and quantified, through use of an LC-MS/MS system, were 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'- cyclo-2'-deoxyguanosine (8,5'cyclodG) and the secondary oxidation products guanidinohydantoin and dehydro-guanidinohydantoin. 8-oxodG and 8,5'cyclodG formed very quickly, reaching a maximum rapidly, but with 8-oxodC a rapid decline occurred thereafter due to its further oxidation into the secondary products, which formed more slowly. Due to the better stability, 8,5'cyclodG correlated better with the general level of oxidation than 8-oxodG. The results emphasize the advantages of measuring other oxidation adducts than 8-oxodG atone. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fenton reaction, hydrogen peroxide, iron sulfate, 8-oxo-7, 8-dihydro-2 '-deoxyguanosine, LC-MS/MS, 8, 5 '-cyclo-2, '-deoxyguanosine, guanidinohydantoin, dehydro-guanidinohydantoin, free radicals, quantification, of modified nucleosides, hydroxyl radical, modified nucleosides, oxidative damage, 2 '-deoxyguanosine
in
Nucleosides, Nucleotides & Nucleic Acids
volume
25
issue
3
pages
259 - 278
publisher
Taylor & Francis
external identifiers
  • wos:000236794200003
  • scopus:33646429061
ISSN
1525-7770
DOI
10.1080/15257770500446956
language
English
LU publication?
yes
id
a52b51c1-a713-4807-a17c-8be33b594560 (old id 413934)
date added to LUP
2007-10-03 10:59:01
date last changed
2019-08-23 02:22:28
@article{a52b51c1-a713-4807-a17c-8be33b594560,
  abstract     = {The influence of incubation time and Fenton reagent concentrations was investigated on the oxidation of 2'-deoxyguanosine. The compounds identified and quantified, through use of an LC-MS/MS system, were 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'- cyclo-2'-deoxyguanosine (8,5'cyclodG) and the secondary oxidation products guanidinohydantoin and dehydro-guanidinohydantoin. 8-oxodG and 8,5'cyclodG formed very quickly, reaching a maximum rapidly, but with 8-oxodC a rapid decline occurred thereafter due to its further oxidation into the secondary products, which formed more slowly. Due to the better stability, 8,5'cyclodG correlated better with the general level of oxidation than 8-oxodG. The results emphasize the advantages of measuring other oxidation adducts than 8-oxodG atone.},
  author       = {Grey, Carl and Adlercreutz, Patrick},
  issn         = {1525-7770},
  keyword      = {Fenton reaction,hydrogen peroxide,iron sulfate,8-oxo-7,8-dihydro-2 '-deoxyguanosine,LC-MS/MS,8,5 '-cyclo-2,'-deoxyguanosine,guanidinohydantoin,dehydro-guanidinohydantoin,free radicals,quantification,of modified nucleosides,hydroxyl radical,modified nucleosides,oxidative damage,2 '-deoxyguanosine},
  language     = {eng},
  number       = {3},
  pages        = {259--278},
  publisher    = {Taylor & Francis},
  series       = {Nucleosides, Nucleotides & Nucleic Acids},
  title        = {Time and concentration dependence of Fenton-induced oxidation of dG},
  url          = {http://dx.doi.org/10.1080/15257770500446956},
  volume       = {25},
  year         = {2006},
}