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Acrylamide affects proliferation and differentiation of the neural progenitor cell line C17.2 and the neuroblastoma cell line SH-SY5Y

Attoff, K.; Kertika, D.; Lundqvist, J.; Oredsson, S. LU and Forsby, A. (2016) In Toxicology in Vitro 35. p.100-111
Abstract

Acrylamide is a well-known neurotoxic compound and people get exposed to the compound by food consumption and environmental pollutants. Since acrylamide crosses the placenta barrier, the fetus is also being exposed resulting in a risk for developmental neurotoxicity. In this study, the neural progenitor cell line C17.2 and the neuroblastoma cell line SH-SY5Y were used to study proliferation and differentiation as alerting indicators for developmental neurotoxicity. For both cell lines, acrylamide reduced the number of viable cells by reducing proliferation and inducing cell death in undifferentiated cells. Acrylamide concentrations starting at 10 fM attenuated the differentiation process in SH-SY5Y cells by sustaining cell proliferation... (More)

Acrylamide is a well-known neurotoxic compound and people get exposed to the compound by food consumption and environmental pollutants. Since acrylamide crosses the placenta barrier, the fetus is also being exposed resulting in a risk for developmental neurotoxicity. In this study, the neural progenitor cell line C17.2 and the neuroblastoma cell line SH-SY5Y were used to study proliferation and differentiation as alerting indicators for developmental neurotoxicity. For both cell lines, acrylamide reduced the number of viable cells by reducing proliferation and inducing cell death in undifferentiated cells. Acrylamide concentrations starting at 10 fM attenuated the differentiation process in SH-SY5Y cells by sustaining cell proliferation and neurite outgrowth was reduced at concentrations from 10 pM. Acrylamide significantly reduced the number of neurons starting at 1 μM and altered the ratio between the different phenotypes in differentiating C17.2 cell cultures. Ten micromolar of acrylamide also reduced the expression of the neuronal and astrocyte biomarkers. Although the neurotoxic concentrations in the femtomolar range seem to be specific for the SH-SY5Y cell line, the fact that micromolar concentrations of acrylamide seem to attenuate the differentiation process in both cell lines raises the interest to further investigations on the possible developmental neurotoxicity of acrylamide.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acrylamide, C17.2, Developmental neurotoxicity, Differentiation, Neural progenitor cells, SH-SY5Y
in
Toxicology in Vitro
volume
35
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:84973320237
  • wos:000380603700013
ISSN
0887-2333
DOI
10.1016/j.tiv.2016.05.014
language
English
LU publication?
yes
id
a6cbf7e5-1ab4-4365-afc9-f88d02ba68f5
date added to LUP
2016-11-23 11:30:20
date last changed
2017-08-27 06:29:41
@article{a6cbf7e5-1ab4-4365-afc9-f88d02ba68f5,
  abstract     = {<p>Acrylamide is a well-known neurotoxic compound and people get exposed to the compound by food consumption and environmental pollutants. Since acrylamide crosses the placenta barrier, the fetus is also being exposed resulting in a risk for developmental neurotoxicity. In this study, the neural progenitor cell line C17.2 and the neuroblastoma cell line SH-SY5Y were used to study proliferation and differentiation as alerting indicators for developmental neurotoxicity. For both cell lines, acrylamide reduced the number of viable cells by reducing proliferation and inducing cell death in undifferentiated cells. Acrylamide concentrations starting at 10 fM attenuated the differentiation process in SH-SY5Y cells by sustaining cell proliferation and neurite outgrowth was reduced at concentrations from 10 pM. Acrylamide significantly reduced the number of neurons starting at 1 μM and altered the ratio between the different phenotypes in differentiating C17.2 cell cultures. Ten micromolar of acrylamide also reduced the expression of the neuronal and astrocyte biomarkers. Although the neurotoxic concentrations in the femtomolar range seem to be specific for the SH-SY5Y cell line, the fact that micromolar concentrations of acrylamide seem to attenuate the differentiation process in both cell lines raises the interest to further investigations on the possible developmental neurotoxicity of acrylamide.</p>},
  author       = {Attoff, K. and Kertika, D. and Lundqvist, J. and Oredsson, S. and Forsby, A.},
  issn         = {0887-2333},
  keyword      = {Acrylamide,C17.2,Developmental neurotoxicity,Differentiation,Neural progenitor cells,SH-SY5Y},
  language     = {eng},
  month        = {09},
  pages        = {100--111},
  publisher    = {Elsevier},
  series       = {Toxicology in Vitro},
  title        = {Acrylamide affects proliferation and differentiation of the neural progenitor cell line C17.2 and the neuroblastoma cell line SH-SY5Y},
  url          = {http://dx.doi.org/10.1016/j.tiv.2016.05.014},
  volume       = {35},
  year         = {2016},
}