Evidence for new catecholamines or related amino acids in some invertebrate neurons
(1977) In Cell and Tissue Research 182. p.525-536- Abstract
- In certain sensory neurons of many different invertebrate species, including the sea anemones. Metridium senile and Tealia felina and the crustacean Anemia salina, fluorophores are formed during the course of the fluorescent histochemical technique of Falck-Hillarp. The presumed catecholamine nature of the neuronal fluorogenic compound was investigated by microspectrofluorometry, and the spectral characteristics of the fluorescence in the taxonomically different species was found to be very similar (excitation maximum at 375 nm with a smaller peak or shoulder at 330 nm and sometimes a shoulder in the spectrum at 410 nm; emission maximum at 475 nm). The emission maximum coincides with that of the catecholamines and DOPA (475 nm). The... (More)
- In certain sensory neurons of many different invertebrate species, including the sea anemones. Metridium senile and Tealia felina and the crustacean Anemia salina, fluorophores are formed during the course of the fluorescent histochemical technique of Falck-Hillarp. The presumed catecholamine nature of the neuronal fluorogenic compound was investigated by microspectrofluorometry, and the spectral characteristics of the fluorescence in the taxonomically different species was found to be very similar (excitation maximum at 375 nm with a smaller peak or shoulder at 330 nm and sometimes a shoulder in the spectrum at 410 nm; emission maximum at 475 nm). The emission maximum coincides with that of the catecholamines and DOPA (475 nm). The excitation maximum (375 nm) directly after formaldehyde treatment, however, differs from that of the catecholamines and DOPA (410 nm), but is similar to the excitation maximum displayed by these catechol derivatives at acid pH. The spectral characteristics of the fluorophore in the sensory cells might therefore theoretically be explained by an acid pH in the cells. This seems improbable, however, and it is suggested that the phenomenon is due to the presence of unknown catechol derivatives. Analyses of the pH-dependent spectral changes indicate that the presumed catechol derivative in Tealia felina is β-hydroxylated, whereas that in Anemia salina is not. (Less)
- Abstract (Swedish)
- In certain sensory neurons of many different invertebrate species, including the sea anemones. Metridium senile and Tealia felina and the crustacean Anemia salina, fluorophores are formed during the course of the fluorescent histochemical technique of Falck-Hillarp. The presumed catecholamine nature of the neuronal fluorogenic compound was investigated by microspectrofluorometry, and the spectral characteristics of the fluorescence in the taxonomically different species was found to be very similar (excitation maximum at 375 nm with a smaller peak or shoulder at 330 nm and sometimes a shoulder in the spectrum at 410 nm; emission maximum at 475 nm). The emission maximum coincides with that of the catecholamines and DOPA (475 nm). The... (More)
- In certain sensory neurons of many different invertebrate species, including the sea anemones. Metridium senile and Tealia felina and the crustacean Anemia salina, fluorophores are formed during the course of the fluorescent histochemical technique of Falck-Hillarp. The presumed catecholamine nature of the neuronal fluorogenic compound was investigated by microspectrofluorometry, and the spectral characteristics of the fluorescence in the taxonomically different species was found to be very similar (excitation maximum at 375 nm with a smaller peak or shoulder at 330 nm and sometimes a shoulder in the spectrum at 410 nm; emission maximum at 475 nm). The emission maximum coincides with that of the catecholamines and DOPA (475 nm). The excitation maximum (375 nm) directly after formaldehyde treatment, however, differs from that of the catecholamines and DOPA (410 nm), but is similar to the excitation maximum displayed by these catechol derivatives at acid pH. The spectral characteristics of the fluorophore in the sensory cells might therefore theoretically be explained by an acid pH in the cells. This seems improbable, however, and it is suggested that the phenomenon is due to the presence of unknown catechol derivatives. Analyses of the pH-dependent spectral changes indicate that the presumed catechol derivative in Tealia felina is β-hydroxylated, whereas that in Anemia salina is not. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/a7ae1375-0719-40cb-8818-90dadf379e31
- author
- Elofsson, Rolf LU ; Falck, Bengt LU ; Lindvall, Olle LU and Myhrberg, Harry
- organization
- publishing date
- 1977
- type
- Contribution to journal
- publication status
- published
- keywords
- Coelenterates, Crustaceans, Microspectrofluorometry
- in
- Cell and Tissue Research
- volume
- 182
- pages
- 525 - 536
- publisher
- Springer
- external identifiers
-
- scopus:0017689417
- ISSN
- 1432-0878
- DOI
- 10.1007/BF00219834
- language
- English
- LU publication?
- yes
- id
- a7ae1375-0719-40cb-8818-90dadf379e31
- date added to LUP
- 2016-11-30 16:32:02
- date last changed
- 2021-01-03 06:24:06
@article{a7ae1375-0719-40cb-8818-90dadf379e31,
abstract = {{In certain sensory neurons of many different invertebrate species, including the sea anemones. Metridium senile and Tealia felina and the crustacean Anemia salina, fluorophores are formed during the course of the fluorescent histochemical technique of Falck-Hillarp. The presumed catecholamine nature of the neuronal fluorogenic compound was investigated by microspectrofluorometry, and the spectral characteristics of the fluorescence in the taxonomically different species was found to be very similar (excitation maximum at 375 nm with a smaller peak or shoulder at 330 nm and sometimes a shoulder in the spectrum at 410 nm; emission maximum at 475 nm). The emission maximum coincides with that of the catecholamines and DOPA (475 nm). The excitation maximum (375 nm) directly after formaldehyde treatment, however, differs from that of the catecholamines and DOPA (410 nm), but is similar to the excitation maximum displayed by these catechol derivatives at acid pH. The spectral characteristics of the fluorophore in the sensory cells might therefore theoretically be explained by an acid pH in the cells. This seems improbable, however, and it is suggested that the phenomenon is due to the presence of unknown catechol derivatives. Analyses of the pH-dependent spectral changes indicate that the presumed catechol derivative in Tealia felina is β-hydroxylated, whereas that in Anemia salina is not.}},
author = {{Elofsson, Rolf and Falck, Bengt and Lindvall, Olle and Myhrberg, Harry}},
issn = {{1432-0878}},
keywords = {{Coelenterates; Crustaceans; Microspectrofluorometry}},
language = {{eng}},
pages = {{525--536}},
publisher = {{Springer}},
series = {{Cell and Tissue Research}},
title = {{Evidence for new catecholamines or related amino acids in some invertebrate neurons}},
url = {{http://dx.doi.org/10.1007/BF00219834}},
doi = {{10.1007/BF00219834}},
volume = {{182}},
year = {{1977}},
}