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Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis.

Gupta, Shashank LU ; Utoft, Regine; Hasseldam, Henrik; Schmidt-Christensen, Anja LU ; Hannibal, Tine LU ; Hansen, Lisbeth LU ; Fransén Pettersson, Nina LU ; Agarwal-Gupta, Noopur; Rozell, Björn and Andersson, Åsa LU , et al. (2013) In PLoS ONE 8(10).
Abstract
Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a... (More)
Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders. (Less)
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PLoS ONE
volume
8
issue
10
publisher
Public Library of Science
external identifiers
  • wos:000325489100102
  • pmid:24124545
  • scopus:84885046957
ISSN
1932-6203
DOI
10.1371/journal.pone.0076330
language
English
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yes
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a849f353-a2ea-4e13-97f4-158c56191c00 (old id 4143267)
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http://www.ncbi.nlm.nih.gov/pubmed/24124545?dopt=Abstract
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2013-11-07 10:16:52
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2019-02-20 05:19:09
@article{a849f353-a2ea-4e13-97f4-158c56191c00,
  abstract     = {Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.},
  articleno    = {e76330},
  author       = {Gupta, Shashank and Utoft, Regine and Hasseldam, Henrik and Schmidt-Christensen, Anja and Hannibal, Tine and Hansen, Lisbeth and Fransén Pettersson, Nina and Agarwal-Gupta, Noopur and Rozell, Björn and Andersson, Åsa and Holmberg, Dan},
  issn         = {1932-6203},
  language     = {eng},
  number       = {10},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis.},
  url          = {http://dx.doi.org/10.1371/journal.pone.0076330},
  volume       = {8},
  year         = {2013},
}