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Effect of membrane composition on DivIVA-membrane interaction

Jurásek, Miroslav ; Flärdh, Klas LU and Vácha, Robert (2019) In Biochimica et Biophysica Acta - Biomembranes
Abstract

DivIVA is a crucial membrane-binding protein that helps to localize other proteins to negatively curved membranes at cellular poles and division septa in Gram-positive bacteria. The N-terminal domain of DivIVA is responsible for membrane binding. However, to which lipids the domain binds or how it recognizes the membrane negative curvature remains elusive. Using computer simulations, we demonstrate that the N-terminal domain of Streptomyces coelicolor DivIVA adsorbs to membranes with affinity and orientation dependent on the lipid composition. The domain interacts non-specifically with lipid phosphates via its arginine-rich tip and the strongest interaction is with cardiolipin. Moreover, we observed a specific attraction between a... (More)

DivIVA is a crucial membrane-binding protein that helps to localize other proteins to negatively curved membranes at cellular poles and division septa in Gram-positive bacteria. The N-terminal domain of DivIVA is responsible for membrane binding. However, to which lipids the domain binds or how it recognizes the membrane negative curvature remains elusive. Using computer simulations, we demonstrate that the N-terminal domain of Streptomyces coelicolor DivIVA adsorbs to membranes with affinity and orientation dependent on the lipid composition. The domain interacts non-specifically with lipid phosphates via its arginine-rich tip and the strongest interaction is with cardiolipin. Moreover, we observed a specific attraction between a negatively charged side patch of the domain and ethanolamine lipids, which addition caused the change of the domain orientation from perpendicular to parallel alignment to the membrane plane. Similar but less electrostatically dependent behavior was observed for the N-terminal domain of Bacillus subtilis. The domain propensity for lipids which prefer negatively curved membranes could be a mechanism for the cellular localization of DivIVA protein.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
DivIVA protein, Lipid membrane, Lipid preference, Molecular dynamics, N-terminal domain, Negative curvature
in
Biochimica et Biophysica Acta - Biomembranes
article number
183144
publisher
Elsevier
external identifiers
  • scopus:85076861655
  • pmid:31821790
ISSN
0005-2736
DOI
10.1016/j.bbamem.2019.183144
language
English
LU publication?
yes
id
a88095f2-a7ad-40ee-a3eb-880ff769fcb3
date added to LUP
2020-01-09 14:57:10
date last changed
2020-01-10 01:55:36
@article{a88095f2-a7ad-40ee-a3eb-880ff769fcb3,
  abstract     = {<p>DivIVA is a crucial membrane-binding protein that helps to localize other proteins to negatively curved membranes at cellular poles and division septa in Gram-positive bacteria. The N-terminal domain of DivIVA is responsible for membrane binding. However, to which lipids the domain binds or how it recognizes the membrane negative curvature remains elusive. Using computer simulations, we demonstrate that the N-terminal domain of Streptomyces coelicolor DivIVA adsorbs to membranes with affinity and orientation dependent on the lipid composition. The domain interacts non-specifically with lipid phosphates via its arginine-rich tip and the strongest interaction is with cardiolipin. Moreover, we observed a specific attraction between a negatively charged side patch of the domain and ethanolamine lipids, which addition caused the change of the domain orientation from perpendicular to parallel alignment to the membrane plane. Similar but less electrostatically dependent behavior was observed for the N-terminal domain of Bacillus subtilis. The domain propensity for lipids which prefer negatively curved membranes could be a mechanism for the cellular localization of DivIVA protein.</p>},
  author       = {Jurásek, Miroslav and Flärdh, Klas and Vácha, Robert},
  issn         = {0005-2736},
  language     = {eng},
  month        = {12},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Biomembranes},
  title        = {Effect of membrane composition on DivIVA-membrane interaction},
  url          = {http://dx.doi.org/10.1016/j.bbamem.2019.183144},
  doi          = {10.1016/j.bbamem.2019.183144},
  year         = {2019},
}