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Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum

Lee, Sang Wook LU ; Laurell, Thomas LU ; Jeong, Ok Chan and Kim, Soyoun (2018) In Biochip Journal 12(1). p.69-74
Abstract

A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 103 orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Macro/nano-structure silicon, Prostate cancer, Prostate-specific antigen, Reverse-phase assay, Sol-gel microarray
in
Biochip Journal
volume
12
issue
1
pages
6 pages
publisher
Korean BioChip Society (KBCS)
external identifiers
  • scopus:85044193601
ISSN
1976-0280
DOI
10.1007/s13206-017-2109-8
language
English
LU publication?
yes
id
a884ddc0-2b18-414a-8797-3cdb79e134f6
date added to LUP
2018-04-05 14:50:29
date last changed
2019-02-26 13:18:29
@article{a884ddc0-2b18-414a-8797-3cdb79e134f6,
  abstract     = {<p>A sol-gel-based reverse-phase microarray was developed with improved sensitivity for prostatespecific antigen (PSA) from serum. The pore-sizecontrolled 3D sol-gel matrix was created with a large surface area to capture target molecules densely. Using the optically active sol-gel nanocomposites, human female serum was spiked with PSA and assessed using the reverse-phase protein microarray. The reverse-phase assay exhibited a limit of detection of 1 pg/mL for PSA and a dynamic range of 10<sup>3</sup> orders of magnitude. Notably, the platform matched the demand of the immunoassay in a simple and feasible manner. Moreover, the platform shortened the total assay time with an increased accuracy of diagnosis.</p>},
  author       = {Lee, Sang Wook and Laurell, Thomas and Jeong, Ok Chan and Kim, Soyoun},
  issn         = {1976-0280},
  keyword      = {Macro/nano-structure silicon,Prostate cancer,Prostate-specific antigen,Reverse-phase assay,Sol-gel microarray},
  language     = {eng},
  month        = {03},
  number       = {1},
  pages        = {69--74},
  publisher    = {Korean BioChip Society (KBCS)},
  series       = {Biochip Journal},
  title        = {Development of a Sol-gel-assisted Reverse-phase Microarray Platform for Simple and Rapid Detection of Prostate-specific Antigen from Serum},
  url          = {http://dx.doi.org/10.1007/s13206-017-2109-8},
  volume       = {12},
  year         = {2018},
}