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Ultra-high-performance supercritical fluid chromatography with quadrupole-time-of-flight mass spectrometry (UHPSFC/QTOF-MS) for analysis of lignin-derived monomeric compounds in processed lignin samples

Prothmann, Jens LU ; Sun, Mingzhe LU ; Spégel, Peter LU ; Sandahl, Margareta LU and Turner, Charlotta LU orcid (2017) In Analytical and Bioanalytical Chemistry 409(30). p.7049-7061
Abstract

The conversion of lignin to potentially high-value low molecular weight compounds often results in complex mixtures of monomeric and oligomeric compounds. In this study, a method for the quantitative and qualitative analysis of 40 lignin-derived compounds using ultra-high-performance supercritical fluid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UHPSFC/QTOF-MS) has been developed. Seven different columns were explored for maximum selectivity. Makeup solvent composition and ion source settings were optimised using a D-optimal design of experiment (DoE). Differently processed lignin samples were analysed and used for the method validation. The new UHPSFC/QTOF-MS method showed good separation of the 40 compounds... (More)

The conversion of lignin to potentially high-value low molecular weight compounds often results in complex mixtures of monomeric and oligomeric compounds. In this study, a method for the quantitative and qualitative analysis of 40 lignin-derived compounds using ultra-high-performance supercritical fluid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UHPSFC/QTOF-MS) has been developed. Seven different columns were explored for maximum selectivity. Makeup solvent composition and ion source settings were optimised using a D-optimal design of experiment (DoE). Differently processed lignin samples were analysed and used for the method validation. The new UHPSFC/QTOF-MS method showed good separation of the 40 compounds within only 6-min retention time, and out of these, 36 showed high ionisation efficiency in negative electrospray ionisation mode. [Figure not available: see fulltext.]

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Column selectivity, Design of experiment, Ionisation efficiency, Lignin, Supercritical fluid chromatography
in
Analytical and Bioanalytical Chemistry
volume
409
issue
30
pages
7049 - 7061
publisher
Springer
external identifiers
  • scopus:85031409367
  • pmid:29030670
  • wos:000417149000010
ISSN
1618-2642
DOI
10.1007/s00216-017-0663-5
language
English
LU publication?
yes
id
a8af10ed-30bf-47fe-9c29-6336ba4b9bde
date added to LUP
2017-10-26 09:06:18
date last changed
2025-01-07 23:26:36
@article{a8af10ed-30bf-47fe-9c29-6336ba4b9bde,
  abstract     = {{<p>The conversion of lignin to potentially high-value low molecular weight compounds often results in complex mixtures of monomeric and oligomeric compounds. In this study, a method for the quantitative and qualitative analysis of 40 lignin-derived compounds using ultra-high-performance supercritical fluid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UHPSFC/QTOF-MS) has been developed. Seven different columns were explored for maximum selectivity. Makeup solvent composition and ion source settings were optimised using a D-optimal design of experiment (DoE). Differently processed lignin samples were analysed and used for the method validation. The new UHPSFC/QTOF-MS method showed good separation of the 40 compounds within only 6-min retention time, and out of these, 36 showed high ionisation efficiency in negative electrospray ionisation mode. [Figure not available: see fulltext.]</p>}},
  author       = {{Prothmann, Jens and Sun, Mingzhe and Spégel, Peter and Sandahl, Margareta and Turner, Charlotta}},
  issn         = {{1618-2642}},
  keywords     = {{Column selectivity; Design of experiment; Ionisation efficiency; Lignin; Supercritical fluid chromatography}},
  language     = {{eng}},
  number       = {{30}},
  pages        = {{7049--7061}},
  publisher    = {{Springer}},
  series       = {{Analytical and Bioanalytical Chemistry}},
  title        = {{Ultra-high-performance supercritical fluid chromatography with quadrupole-time-of-flight mass spectrometry (UHPSFC/QTOF-MS) for analysis of lignin-derived monomeric compounds in processed lignin samples}},
  url          = {{http://dx.doi.org/10.1007/s00216-017-0663-5}},
  doi          = {{10.1007/s00216-017-0663-5}},
  volume       = {{409}},
  year         = {{2017}},
}