Protein phosphorylation/dephosphorylation in the inner membrane of potato tuber mitochondria
(2000) In FEBS Letters 475(3). p.213-217- Abstract
Inside-out inner mitochondrial membranes free of matrix proteins were isolated from purified potato tuber (Solanum tuberosum L.) mitochondria and incubated with [γ-32P]ATP. Proteins were separated by SDS-PAGE and visualized by autoradiography. Phosphorylation of inner membrane proteins, including ATPase subunits, was strongly inhibited by the phosphoprotein phosphatase inhibitor NaF. We propose that an inner membrane phosphoprotein phosphatase is required for activation of the inner membrane protein kinase. When prelabelled inner membranes were incubated in the absence of [γ- 32P]ATP, there was no phosphoprotein dephosphorylation unless a soluble matrix fraction was added. This dephosphorylation was inhibited by... (More)
Inside-out inner mitochondrial membranes free of matrix proteins were isolated from purified potato tuber (Solanum tuberosum L.) mitochondria and incubated with [γ-32P]ATP. Proteins were separated by SDS-PAGE and visualized by autoradiography. Phosphorylation of inner membrane proteins, including ATPase subunits, was strongly inhibited by the phosphoprotein phosphatase inhibitor NaF. We propose that an inner membrane phosphoprotein phosphatase is required for activation of the inner membrane protein kinase. When prelabelled inner membranes were incubated in the absence of [γ- 32P]ATP, there was no phosphoprotein dephosphorylation unless a soluble matrix fraction was added. This dephosphorylation was inhibited by NaF, but not by okadaic acid. We conclude that the mitochondrial matrix contains a phosphoprotein phosphatase that is responsible for dephosphorylation of inner membrane phosphoproteins. (C) 2000 Federation of European Biochemical Societies.
(Less)
- author
- Struglics, André LU ; Fredlund, Kenneth M. ; Konstantinov, Yuri M. ; Allen, John F. LU and Møller, Ian M.
- organization
- publishing date
- 2000-06-23
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- (Plant) mitochondrion, Inner membrane, Matrix, Protein kinase, Protein kinase phosphatase, Protein phosphatase, Protein phosphorylation
- in
- FEBS Letters
- volume
- 475
- issue
- 3
- pages
- 213 - 217
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:10869559
- scopus:0034705703
- ISSN
- 0014-5793
- DOI
- 10.1016/S0014-5793(00)01680-X
- language
- English
- LU publication?
- yes
- id
- a9ba1f8e-a445-4408-952d-8cdde743a0b6
- date added to LUP
- 2016-10-14 13:07:55
- date last changed
- 2024-01-04 14:24:42
@article{a9ba1f8e-a445-4408-952d-8cdde743a0b6, abstract = {{<p>Inside-out inner mitochondrial membranes free of matrix proteins were isolated from purified potato tuber (Solanum tuberosum L.) mitochondria and incubated with [γ-<sup>32</sup>P]ATP. Proteins were separated by SDS-PAGE and visualized by autoradiography. Phosphorylation of inner membrane proteins, including ATPase subunits, was strongly inhibited by the phosphoprotein phosphatase inhibitor NaF. We propose that an inner membrane phosphoprotein phosphatase is required for activation of the inner membrane protein kinase. When prelabelled inner membranes were incubated in the absence of [γ- <sup>32</sup>P]ATP, there was no phosphoprotein dephosphorylation unless a soluble matrix fraction was added. This dephosphorylation was inhibited by NaF, but not by okadaic acid. We conclude that the mitochondrial matrix contains a phosphoprotein phosphatase that is responsible for dephosphorylation of inner membrane phosphoproteins. (C) 2000 Federation of European Biochemical Societies.</p>}}, author = {{Struglics, André and Fredlund, Kenneth M. and Konstantinov, Yuri M. and Allen, John F. and Møller, Ian M.}}, issn = {{0014-5793}}, keywords = {{(Plant) mitochondrion; Inner membrane; Matrix; Protein kinase; Protein kinase phosphatase; Protein phosphatase; Protein phosphorylation}}, language = {{eng}}, month = {{06}}, number = {{3}}, pages = {{213--217}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Protein phosphorylation/dephosphorylation in the inner membrane of potato tuber mitochondria}}, url = {{http://dx.doi.org/10.1016/S0014-5793(00)01680-X}}, doi = {{10.1016/S0014-5793(00)01680-X}}, volume = {{475}}, year = {{2000}}, }