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Protein phosphorylation/dephosphorylation in the inner membrane of potato tuber mitochondria

Struglics, André LU ; Fredlund, Kenneth M.; Konstantinov, Yuri M.; Allen, John F. LU and Møller, Ian M. (2000) In FEBS Letters 475(3). p.213-217
Abstract

Inside-out inner mitochondrial membranes free of matrix proteins were isolated from purified potato tuber (Solanum tuberosum L.) mitochondria and incubated with [γ-32P]ATP. Proteins were separated by SDS-PAGE and visualized by autoradiography. Phosphorylation of inner membrane proteins, including ATPase subunits, was strongly inhibited by the phosphoprotein phosphatase inhibitor NaF. We propose that an inner membrane phosphoprotein phosphatase is required for activation of the inner membrane protein kinase. When prelabelled inner membranes were incubated in the absence of [γ- 32P]ATP, there was no phosphoprotein dephosphorylation unless a soluble matrix fraction was added. This dephosphorylation was inhibited by... (More)

Inside-out inner mitochondrial membranes free of matrix proteins were isolated from purified potato tuber (Solanum tuberosum L.) mitochondria and incubated with [γ-32P]ATP. Proteins were separated by SDS-PAGE and visualized by autoradiography. Phosphorylation of inner membrane proteins, including ATPase subunits, was strongly inhibited by the phosphoprotein phosphatase inhibitor NaF. We propose that an inner membrane phosphoprotein phosphatase is required for activation of the inner membrane protein kinase. When prelabelled inner membranes were incubated in the absence of [γ- 32P]ATP, there was no phosphoprotein dephosphorylation unless a soluble matrix fraction was added. This dephosphorylation was inhibited by NaF, but not by okadaic acid. We conclude that the mitochondrial matrix contains a phosphoprotein phosphatase that is responsible for dephosphorylation of inner membrane phosphoproteins. (C) 2000 Federation of European Biochemical Societies.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
(Plant) mitochondrion, Inner membrane, Matrix, Protein kinase, Protein kinase phosphatase, Protein phosphatase, Protein phosphorylation
in
FEBS Letters
volume
475
issue
3
pages
213 - 217
publisher
Wiley-Blackwell
external identifiers
  • scopus:0034705703
ISSN
0014-5793
DOI
10.1016/S0014-5793(00)01680-X
language
English
LU publication?
yes
id
a9ba1f8e-a445-4408-952d-8cdde743a0b6
date added to LUP
2016-10-14 13:07:55
date last changed
2017-06-28 14:25:41
@article{a9ba1f8e-a445-4408-952d-8cdde743a0b6,
  abstract     = {<p>Inside-out inner mitochondrial membranes free of matrix proteins were isolated from purified potato tuber (Solanum tuberosum L.) mitochondria and incubated with [γ-<sup>32</sup>P]ATP. Proteins were separated by SDS-PAGE and visualized by autoradiography. Phosphorylation of inner membrane proteins, including ATPase subunits, was strongly inhibited by the phosphoprotein phosphatase inhibitor NaF. We propose that an inner membrane phosphoprotein phosphatase is required for activation of the inner membrane protein kinase. When prelabelled inner membranes were incubated in the absence of [γ- <sup>32</sup>P]ATP, there was no phosphoprotein dephosphorylation unless a soluble matrix fraction was added. This dephosphorylation was inhibited by NaF, but not by okadaic acid. We conclude that the mitochondrial matrix contains a phosphoprotein phosphatase that is responsible for dephosphorylation of inner membrane phosphoproteins. (C) 2000 Federation of European Biochemical Societies.</p>},
  author       = {Struglics, André and Fredlund, Kenneth M. and Konstantinov, Yuri M. and Allen, John F. and Møller, Ian M.},
  issn         = {0014-5793},
  keyword      = {(Plant) mitochondrion,Inner membrane,Matrix,Protein kinase,Protein kinase phosphatase,Protein phosphatase,Protein phosphorylation},
  language     = {eng},
  month        = {06},
  number       = {3},
  pages        = {213--217},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Protein phosphorylation/dephosphorylation in the inner membrane of potato tuber mitochondria},
  url          = {http://dx.doi.org/10.1016/S0014-5793(00)01680-X},
  volume       = {475},
  year         = {2000},
}