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Lipase action on a monoolein/sodium oleate aqueous cubic liquid crystalline phase - a NMR and X-ray diffraction study

Caboi, F ; Borné, Johanna LU ; Nylander, Tommy LU ; Khan, Ali LU ; Svendsen, A and Patkar, S (2002) In Colloids and Surfaces B: Biointerfaces 26(1-2). p.159-171
Abstract
The effect of adding Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a monoolein (MO)/sodium oleate (NaO) aqueous cubic liquid crystalline phase has been studied. H-1-NMR, C-13-NMR, H-1-PGSE (Pulsed-magnetic field Gradient Spin-Echo) self-diffusion measurements, and Small Angle X-ray Diffraction were used to follow the degradation of the cubic phase. The reaction sequence in terms of phase transitions follows the order bicontinuous cubic --> reverse hexagonal --> micellar cubic --> micellar phase and corresponds to the previously determined phase diagrams. These changes correlate with changes in the lipid composition observed by C-13-NMR and confirmed by HPLC analysis. The initial decrease of the diffusion coefficients... (More)
The effect of adding Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a monoolein (MO)/sodium oleate (NaO) aqueous cubic liquid crystalline phase has been studied. H-1-NMR, C-13-NMR, H-1-PGSE (Pulsed-magnetic field Gradient Spin-Echo) self-diffusion measurements, and Small Angle X-ray Diffraction were used to follow the degradation of the cubic phase. The reaction sequence in terms of phase transitions follows the order bicontinuous cubic --> reverse hexagonal --> micellar cubic --> micellar phase and corresponds to the previously determined phase diagrams. These changes correlate with changes in the lipid composition observed by C-13-NMR and confirmed by HPLC analysis. The initial decrease of the diffusion coefficients of water and lipid can be related to the transformation of the bicontinuous cubic phase to a reverse hexagonal structure. (C) 2002 Elsevier Science B.V. All rights reserved. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
self-assembly structure, lipolysis, cubic phase, lipase, monoolein
in
Colloids and Surfaces B: Biointerfaces
volume
26
issue
1-2
pages
159 - 171
publisher
Elsevier
external identifiers
  • wos:000179487200016
  • scopus:0036074332
ISSN
1873-4367
DOI
10.1016/S0927-7765(02)00035-8
language
English
LU publication?
yes
id
aa29a0c6-0751-4b95-badd-9ecb8325b69b (old id 322664)
date added to LUP
2016-04-01 16:29:27
date last changed
2025-10-14 10:02:07
@article{aa29a0c6-0751-4b95-badd-9ecb8325b69b,
  abstract     = {{The effect of adding Thermomyces (formerly Humicola) lanuginosa lipase (TLL) to a monoolein (MO)/sodium oleate (NaO) aqueous cubic liquid crystalline phase has been studied. H-1-NMR, C-13-NMR, H-1-PGSE (Pulsed-magnetic field Gradient Spin-Echo) self-diffusion measurements, and Small Angle X-ray Diffraction were used to follow the degradation of the cubic phase. The reaction sequence in terms of phase transitions follows the order bicontinuous cubic --> reverse hexagonal --> micellar cubic --> micellar phase and corresponds to the previously determined phase diagrams. These changes correlate with changes in the lipid composition observed by C-13-NMR and confirmed by HPLC analysis. The initial decrease of the diffusion coefficients of water and lipid can be related to the transformation of the bicontinuous cubic phase to a reverse hexagonal structure. (C) 2002 Elsevier Science B.V. All rights reserved.}},
  author       = {{Caboi, F and Borné, Johanna and Nylander, Tommy and Khan, Ali and Svendsen, A and Patkar, S}},
  issn         = {{1873-4367}},
  keywords     = {{self-assembly structure; lipolysis; cubic phase; lipase; monoolein}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{159--171}},
  publisher    = {{Elsevier}},
  series       = {{Colloids and Surfaces B: Biointerfaces}},
  title        = {{Lipase action on a monoolein/sodium oleate aqueous cubic liquid crystalline phase - a NMR and X-ray diffraction study}},
  url          = {{http://dx.doi.org/10.1016/S0927-7765(02)00035-8}},
  doi          = {{10.1016/S0927-7765(02)00035-8}},
  volume       = {{26}},
  year         = {{2002}},
}