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Cation and buffer specific effects on the DNA-lipid interaction

Mura, Monica ; Humphreys, Ben LU ; Gilbert, Jennifer LU orcid ; Salis, Andrea and Nylander, Tommy LU (2023) In Colloids and Surfaces B: Biointerfaces 223.
Abstract
Knowledge of DNA - lipid layer interactions is key for the development of biosensors, synthetic nanopores, scaffolds, and gene-delivery systems. These interactions are strongly affected by the ionic composition of the solvent. We have combined quartz crystal microbalance (QCM) and ellipsometry measurements to reveal how pH, buffers and alkali metal chloride salts affect the interaction of DNA with lipid bilayers (DOTAP/DOPC 30:70 in moles). We found that the thickness of the DNA layer adsorbed onto the lipid bilayer decreased in the order citrate > phosphate > Tris > HEPES. The effect of cations on the thickness of the DNA layer decreased in the order (K+ > Na+ > Cs+ ∼ Li+).... (More)
Knowledge of DNA - lipid layer interactions is key for the development of biosensors, synthetic nanopores, scaffolds, and gene-delivery systems. These interactions are strongly affected by the ionic composition of the solvent. We have combined quartz crystal microbalance (QCM) and ellipsometry measurements to reveal how pH, buffers and alkali metal chloride salts affect the interaction of DNA with lipid bilayers (DOTAP/DOPC 30:70 in moles). We found that the thickness of the DNA layer adsorbed onto the lipid bilayer decreased in the order citrate > phosphate > Tris > HEPES. The effect of cations on the thickness of the DNA layer decreased in the order (K+ > Na+ > Cs+ ∼ Li+). Rationalization of the experimental results requires that adsorption, due to cation specific charge screening, is driven by the simultaneous action of two mechanisms namely, the law of matching water affinities for kosmotropes (Li+) and ion dispersion forces for chaotropes (Cs+). The outcome of these two opposing mechanisms is a “bell-shaped” specific cations sequence. Moreover, a superimposed buffer specificity, which goes beyond the simple effect of pH regulation, further modulated cation specificity. In summary, DNA-lipid bilayer interactions are maximized if citrate buffer (50 mM, pH 7.4) and KCl (100 mM) are used. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces B: Biointerfaces
volume
223
article number
113187
publisher
Elsevier
external identifiers
  • scopus:85149783441
  • pmid:36739672
ISSN
0927-7765
DOI
10.1016/j.colsurfb.2023.113187
language
English
LU publication?
yes
id
ab67ee7c-5645-4890-b6bb-abaf649db4fd
date added to LUP
2023-05-10 03:29:14
date last changed
2023-11-08 05:46:07
@article{ab67ee7c-5645-4890-b6bb-abaf649db4fd,
  abstract     = {{Knowledge of DNA - lipid layer interactions is key for the development of biosensors, synthetic nanopores, scaffolds, and gene-delivery systems. These interactions are strongly affected by the ionic composition of the solvent. We have combined quartz crystal microbalance (QCM) and ellipsometry measurements to reveal how pH, buffers and alkali metal chloride salts affect the interaction of DNA with lipid bilayers (DOTAP/DOPC 30:70 in moles). We found that the thickness of the DNA layer adsorbed onto the lipid bilayer decreased in the order citrate &gt; phosphate &gt; Tris &gt; HEPES. The effect of cations on the thickness of the DNA layer decreased in the order (K<sup>+</sup> &gt; Na<sup>+</sup> &gt; Cs<sup>+</sup> ∼ Li<sup>+</sup>). Rationalization of the experimental results requires that adsorption, due to cation specific charge screening, is driven by the simultaneous action of two mechanisms namely, the law of matching water affinities for kosmotropes (Li<sup>+</sup>) and ion dispersion forces for chaotropes (Cs<sup>+</sup>). The outcome of these two opposing mechanisms is a “bell-shaped” specific cations sequence. Moreover, a superimposed buffer specificity, which goes beyond the simple effect of pH regulation, further modulated cation specificity. In summary, DNA-lipid bilayer interactions are maximized if citrate buffer (50 mM, pH 7.4) and KCl (100 mM) are used.}},
  author       = {{Mura, Monica and Humphreys, Ben and Gilbert, Jennifer and Salis, Andrea and Nylander, Tommy}},
  issn         = {{0927-7765}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Colloids and Surfaces B: Biointerfaces}},
  title        = {{Cation and buffer specific effects on the DNA-lipid interaction}},
  url          = {{http://dx.doi.org/10.1016/j.colsurfb.2023.113187}},
  doi          = {{10.1016/j.colsurfb.2023.113187}},
  volume       = {{223}},
  year         = {{2023}},
}