Dual modulation of neuron-specific microRNAs and the REST complex promotes functional maturation of human adult induced neurons
(2019) In FEBS Letters 593(23). p.3370-3380- Abstract
Direct neuronal reprogramming can be achieved using different approaches: by expressing neuronal transcription factors or microRNAs; and by knocking down neuronal repressive elements. However, there still exists a high variability in terms of the quality and maturity of the induced neurons obtained, depending on the reprogramming strategy employed. Here, we evaluate different long-term culture conditions and study the effect of expressing the neuronal-specific microRNAs, miR124 and miR9/9*, while reprogramming with forced expression of the transcription factors Ascl1, Brn2, and knockdown of the neuronal repressor REST. We show that the addition of microRNAs supports neuronal maturation in terms of gene and protein expression, as well as... (More)
Direct neuronal reprogramming can be achieved using different approaches: by expressing neuronal transcription factors or microRNAs; and by knocking down neuronal repressive elements. However, there still exists a high variability in terms of the quality and maturity of the induced neurons obtained, depending on the reprogramming strategy employed. Here, we evaluate different long-term culture conditions and study the effect of expressing the neuronal-specific microRNAs, miR124 and miR9/9*, while reprogramming with forced expression of the transcription factors Ascl1, Brn2, and knockdown of the neuronal repressor REST. We show that the addition of microRNAs supports neuronal maturation in terms of gene and protein expression, as well as in terms of electrophysiological properties.
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- author
- Birtele, Marcella LU ; Sharma, Yogita LU ; Kidnapillai, Srisaiyini LU ; Lau, Shong LU ; Stoker, Thomas B. ; Barker, Roger A. LU ; Rylander Ottosson, Daniella LU ; Drouin-Ouellet, Janelle LU and Parmar, Malin LU
- organization
- publishing date
- 2019-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- direct reprogramming, electrophysiology, human adult neurons, induced neurons, miR124, miR9
- in
- FEBS Letters
- volume
- 593
- issue
- 23
- pages
- 11 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:85074443776
- pmid:31535361
- ISSN
- 0014-5793
- DOI
- 10.1002/1873-3468.13612
- language
- English
- LU publication?
- yes
- id
- ac8505d5-aa1e-48f1-8dec-abe86fa9a4dc
- date added to LUP
- 2019-11-22 12:36:42
- date last changed
- 2024-04-16 23:51:06
@article{ac8505d5-aa1e-48f1-8dec-abe86fa9a4dc, abstract = {{<p>Direct neuronal reprogramming can be achieved using different approaches: by expressing neuronal transcription factors or microRNAs; and by knocking down neuronal repressive elements. However, there still exists a high variability in terms of the quality and maturity of the induced neurons obtained, depending on the reprogramming strategy employed. Here, we evaluate different long-term culture conditions and study the effect of expressing the neuronal-specific microRNAs, miR124 and miR9/9*, while reprogramming with forced expression of the transcription factors Ascl1, Brn2, and knockdown of the neuronal repressor REST. We show that the addition of microRNAs supports neuronal maturation in terms of gene and protein expression, as well as in terms of electrophysiological properties.</p>}}, author = {{Birtele, Marcella and Sharma, Yogita and Kidnapillai, Srisaiyini and Lau, Shong and Stoker, Thomas B. and Barker, Roger A. and Rylander Ottosson, Daniella and Drouin-Ouellet, Janelle and Parmar, Malin}}, issn = {{0014-5793}}, keywords = {{direct reprogramming; electrophysiology; human adult neurons; induced neurons; miR124; miR9}}, language = {{eng}}, number = {{23}}, pages = {{3370--3380}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Dual modulation of neuron-specific microRNAs and the REST complex promotes functional maturation of human adult induced neurons}}, url = {{http://dx.doi.org/10.1002/1873-3468.13612}}, doi = {{10.1002/1873-3468.13612}}, volume = {{593}}, year = {{2019}}, }