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In vitro methods to study colon release : State of the art and an outlook on new strategies for better in-vitro biorelevant release media

Wahlgren, Marie LU ; Axenstrand, Magdalena LU ; Håkansson, Åsa LU ; Marefati, Ali LU and Pedersen, Betty Lomstein (2019) In Pharmaceutics 11(2).
Abstract


The primary focus of this review is a discussion regarding in vitro media for colon release, but we also give a brief overview of colon delivery and the colon microbiota as a baseline for this discussion. The large intestine is colonized by a vast number of bacteria, approximately 10
12
per gram of intestinal content. The microbial community in the colon is complex and there is still much that is unknown about its composition and the activity of the microbiome. However, it is evident that this complex microbiota will affect the release from... (More)


The primary focus of this review is a discussion regarding in vitro media for colon release, but we also give a brief overview of colon delivery and the colon microbiota as a baseline for this discussion. The large intestine is colonized by a vast number of bacteria, approximately 10
12
per gram of intestinal content. The microbial community in the colon is complex and there is still much that is unknown about its composition and the activity of the microbiome. However, it is evident that this complex microbiota will affect the release from oral formulations targeting the colon. This includes the release of active drug substances, food supplements, and live microorganisms, such as probiotic bacteria and bacteria used for microbiota transplantations. Currently, there are no standardized colon release media, but researchers employ in vitro models representing the colon ranging from reasonable simple systems with adjusted pH with or without key enzymes to the use of fecal samples. In this review, we present the pros and cons for different existing in vitro models. Furthermore, we summarize the current knowledge of the colonic microbiota composition which is of importance to the fermentation capacity of carbohydrates and suggest a strategy to choose bacteria for a new more standardized in vitro dissolution medium for the colon.

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Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Colon delivery, Colon microbiota, In vitro systems
in
Pharmaceutics
volume
11
issue
2
article number
95
publisher
MDPI AG
external identifiers
  • scopus:85063381561
  • pmid:30813323
ISSN
1999-4923
DOI
10.3390/pharmaceutics11020095
language
English
LU publication?
yes
id
acd85bcc-cc9f-4cf3-9eda-5ec1079b4137
date added to LUP
2019-04-08 12:29:08
date last changed
2020-10-25 05:45:19
@article{acd85bcc-cc9f-4cf3-9eda-5ec1079b4137,
  abstract     = {<p><br>
                                                         The primary focus of this review is a discussion regarding in vitro media for colon release, but we also give a brief overview of colon delivery and the colon microbiota as a baseline for this discussion. The large intestine is colonized by a vast number of bacteria, approximately 10                             <br>
                            <sup>12</sup><br>
                                                          per gram of intestinal content. The microbial community in the colon is complex and there is still much that is unknown about its composition and the activity of the microbiome. However, it is evident that this complex microbiota will affect the release from oral formulations targeting the colon. This includes the release of active drug substances, food supplements, and live microorganisms, such as probiotic bacteria and bacteria used for microbiota transplantations. Currently, there are no standardized colon release media, but researchers employ in vitro models representing the colon ranging from reasonable simple systems with adjusted pH with or without key enzymes to the use of fecal samples. In this review, we present the pros and cons for different existing in vitro models. Furthermore, we summarize the current knowledge of the colonic microbiota composition which is of importance to the fermentation capacity of carbohydrates and suggest a strategy to choose bacteria for a new more standardized in vitro dissolution medium for the colon.                         <br>
                        </p>},
  author       = {Wahlgren, Marie and Axenstrand, Magdalena and Håkansson, Åsa and Marefati, Ali and Pedersen, Betty Lomstein},
  issn         = {1999-4923},
  language     = {eng},
  month        = {02},
  number       = {2},
  publisher    = {MDPI AG},
  series       = {Pharmaceutics},
  title        = {In vitro methods to study colon release : State of the art and an outlook on new strategies for better in-vitro biorelevant release media},
  url          = {http://dx.doi.org/10.3390/pharmaceutics11020095},
  doi          = {10.3390/pharmaceutics11020095},
  volume       = {11},
  year         = {2019},
}