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Early Metabolic Flare in Squamous Cell Carcinoma after Chemotherapy is a Marker of Treatment Sensitivity In Vitro

Bjurberg, Maria LU ; Abedinpour, Parisa LU ; Brun, Eva LU ; Baldetorp, Bo LU ; Borgström, Per; Wennerberg, Johan LU and Kjellén, Elisabeth LU (2010) In Nuclear medicine and molecular imaging 44(3). p.165-169
Abstract

PURPOSE: Early metabolic response with a decrease in glucose demand after cytotoxic treatment has been reported to precede tumor volume shrinkage. However, preclinical studies report of a very early rise in metabolism, a flare, following treatment. To elucidate these observations, we performed an experimental study on early metabolic response with sequential analysis of metabolic changes. METHODS: Three squamous cell carcinoma cell lines and one nontumorigenic cell line were exposed to cisplatin. The uptake of the fluorescent glucose analogue 2-NBDG was examined at days 1-6 using fluorescence microscopy. The relation between 2-NBDG-uptake and cell survival was evaluated. RESULTS: The tumor cells exhibited a high uptake of 2-NBDG,... (More)

PURPOSE: Early metabolic response with a decrease in glucose demand after cytotoxic treatment has been reported to precede tumor volume shrinkage. However, preclinical studies report of a very early rise in metabolism, a flare, following treatment. To elucidate these observations, we performed an experimental study on early metabolic response with sequential analysis of metabolic changes. METHODS: Three squamous cell carcinoma cell lines and one nontumorigenic cell line were exposed to cisplatin. The uptake of the fluorescent glucose analogue 2-NBDG was examined at days 1-6 using fluorescence microscopy. The relation between 2-NBDG-uptake and cell survival was evaluated. RESULTS: The tumor cells exhibited a high uptake of 2-NBDG, whereas the uptake in the nonmalignant cells was low. The more cisplatin sensitive cell lines exhibited a more pronounced metabolic flare than the less sensitive cell line. CONCLUSION: A metabolic flare was a very early sign of treatment response and potentially it could be used as an early marker of treatment sensitivity.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nuclear medicine and molecular imaging
volume
44
issue
3
pages
5 pages
publisher
Springer Verlag
external identifiers
  • scopus:79952738194
ISSN
1869-3474
DOI
10.1007/s13139-010-0034-z
language
English
LU publication?
yes
id
ace82896-7bbf-42c7-a036-6a6f1f4e7eaa
alternative location
https://europepmc.org/abstract/pmc/pmc2987232
date added to LUP
2019-07-01 14:17:12
date last changed
2019-08-13 18:02:06
@article{ace82896-7bbf-42c7-a036-6a6f1f4e7eaa,
  abstract     = {<p>PURPOSE: Early metabolic response with a decrease in glucose demand after cytotoxic treatment has been reported to precede tumor volume shrinkage. However, preclinical studies report of a very early rise in metabolism, a flare, following treatment. To elucidate these observations, we performed an experimental study on early metabolic response with sequential analysis of metabolic changes. METHODS: Three squamous cell carcinoma cell lines and one nontumorigenic cell line were exposed to cisplatin. The uptake of the fluorescent glucose analogue 2-NBDG was examined at days 1-6 using fluorescence microscopy. The relation between 2-NBDG-uptake and cell survival was evaluated. RESULTS: The tumor cells exhibited a high uptake of 2-NBDG, whereas the uptake in the nonmalignant cells was low. The more cisplatin sensitive cell lines exhibited a more pronounced metabolic flare than the less sensitive cell line. CONCLUSION: A metabolic flare was a very early sign of treatment response and potentially it could be used as an early marker of treatment sensitivity.</p>},
  author       = {Bjurberg, Maria and Abedinpour, Parisa and Brun, Eva and Baldetorp, Bo and Borgström, Per and Wennerberg, Johan and Kjellén, Elisabeth},
  issn         = {1869-3474},
  language     = {eng},
  number       = {3},
  pages        = {165--169},
  publisher    = {Springer Verlag},
  series       = {Nuclear medicine and molecular imaging},
  title        = {Early Metabolic Flare in Squamous Cell Carcinoma after Chemotherapy is a Marker of Treatment Sensitivity In Vitro},
  url          = {http://dx.doi.org/10.1007/s13139-010-0034-z},
  volume       = {44},
  year         = {2010},
}