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MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC

Enes, Sara Rolandsson LU ; Sjöland, Annika Andersson LU ; Skog, Ingrid LU ; Hansson, Lennart LU ; Larsson, Hillevi LU ; Le Blanc, Katarina; Eriksson, Leif LU ; Bjermer, Leif LU ; Scheding, Stefan LU and Westergren-Thorsson, Gunilla LU (2016) In Scientific Reports 6.
Abstract

Mesenchymal stromal cells (MSC) are multipotent cells with regenerative and immune-modulatory properties. Therefore, MSC have been proposed as a potential cell-therapy for bronchiolitis obliterans syndrome (BOS). On the other hand, there are publications demonstrating that MSC might be involved in the development of BOS. Despite limited knowledge regarding the functional role of tissue-resident lung-MSC, several clinical trials have been performed using MSC, particularly bone marrow (BM)-derived MSC, for various lung diseases. We aimed to compare lung-MSC with the well-characterized BM-MSC. Furthermore, MSC isolated from lung-transplanted patients with BOS were compared to patients without BOS. Our study show that lung-MSCs are smaller,... (More)

Mesenchymal stromal cells (MSC) are multipotent cells with regenerative and immune-modulatory properties. Therefore, MSC have been proposed as a potential cell-therapy for bronchiolitis obliterans syndrome (BOS). On the other hand, there are publications demonstrating that MSC might be involved in the development of BOS. Despite limited knowledge regarding the functional role of tissue-resident lung-MSC, several clinical trials have been performed using MSC, particularly bone marrow (BM)-derived MSC, for various lung diseases. We aimed to compare lung-MSC with the well-characterized BM-MSC. Furthermore, MSC isolated from lung-transplanted patients with BOS were compared to patients without BOS. Our study show that lung-MSCs are smaller, possess a higher colony-forming capacity and have a different cytokine profile compared to BM-MSC. Utilizing gene expression profiling, 89 genes including lung-specific FOXF1 and HOXB5 were found to be significantly different between BM-MSC and lung-MSC. No significant differences in cytokine secretion or gene expression were found between MSC isolated from BOS patients compared recipients without BOS. These data demonstrate that lung-resident MSC possess lung-specific properties. Furthermore, these results show that MSC isolated from lung-transplanted patients with BOS do not have an altered phenotype compared to MSC isolated from good outcome recipients.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
6
publisher
Nature Publishing Group
external identifiers
  • scopus:84977267137
  • wos:000379181500001
ISSN
2045-2322
DOI
10.1038/srep29160
language
English
LU publication?
yes
id
afd58849-6375-4563-bfc8-5d82dea4dc42
date added to LUP
2016-07-26 08:42:10
date last changed
2017-09-03 05:11:00
@article{afd58849-6375-4563-bfc8-5d82dea4dc42,
  abstract     = {<p>Mesenchymal stromal cells (MSC) are multipotent cells with regenerative and immune-modulatory properties. Therefore, MSC have been proposed as a potential cell-therapy for bronchiolitis obliterans syndrome (BOS). On the other hand, there are publications demonstrating that MSC might be involved in the development of BOS. Despite limited knowledge regarding the functional role of tissue-resident lung-MSC, several clinical trials have been performed using MSC, particularly bone marrow (BM)-derived MSC, for various lung diseases. We aimed to compare lung-MSC with the well-characterized BM-MSC. Furthermore, MSC isolated from lung-transplanted patients with BOS were compared to patients without BOS. Our study show that lung-MSCs are smaller, possess a higher colony-forming capacity and have a different cytokine profile compared to BM-MSC. Utilizing gene expression profiling, 89 genes including lung-specific FOXF1 and HOXB5 were found to be significantly different between BM-MSC and lung-MSC. No significant differences in cytokine secretion or gene expression were found between MSC isolated from BOS patients compared recipients without BOS. These data demonstrate that lung-resident MSC possess lung-specific properties. Furthermore, these results show that MSC isolated from lung-transplanted patients with BOS do not have an altered phenotype compared to MSC isolated from good outcome recipients.</p>},
  articleno    = {29160},
  author       = {Enes, Sara Rolandsson and Sjöland, Annika Andersson and Skog, Ingrid and Hansson, Lennart and Larsson, Hillevi and Le Blanc, Katarina and Eriksson, Leif and Bjermer, Leif and Scheding, Stefan and Westergren-Thorsson, Gunilla},
  issn         = {2045-2322},
  language     = {eng},
  month        = {07},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC},
  url          = {http://dx.doi.org/10.1038/srep29160},
  volume       = {6},
  year         = {2016},
}