Quantitative Time-Resolved Fluorescence Imaging of Androgen Receptor and Prostate-Specific Antigen in Prostate Tissue Sections
(2016) In Journal of Histochemistry and Cytochemistry 64(5). p.311-322- Abstract
Androgen receptor (AR) and prostate-specific antigen (PSA) are expressed in the prostate and are involved in prostate cancer (PCa). The aim of this study was to develop reliable protocols for reproducible quantification of AR and PSA in benign and malignant prostate tissue using time-resolved fluorescence (TRF) imaging techniques. AR and PSA were detected with TRF in tissue microarrays from 91 PCa patients. p63/ alpha-methylacyl-CoA racemase (AMACR) staining on consecutive sections was used to categorize tissue areas as benign or cancerous. Automated image analysis was used to quantify staining intensity. AR intensity was significantly higher in AMACR+ and lower in AMACR- cancer areas as compared with benign epithelium. The PSA... (More)
Androgen receptor (AR) and prostate-specific antigen (PSA) are expressed in the prostate and are involved in prostate cancer (PCa). The aim of this study was to develop reliable protocols for reproducible quantification of AR and PSA in benign and malignant prostate tissue using time-resolved fluorescence (TRF) imaging techniques. AR and PSA were detected with TRF in tissue microarrays from 91 PCa patients. p63/ alpha-methylacyl-CoA racemase (AMACR) staining on consecutive sections was used to categorize tissue areas as benign or cancerous. Automated image analysis was used to quantify staining intensity. AR intensity was significantly higher in AMACR+ and lower in AMACR- cancer areas as compared with benign epithelium. The PSA intensity was significantly lower in cancer areas, particularly in AMACR- glands. The AR/PSA ratio varied significantly in the AMACR+ tumor cells as compared with benign glands. There was a trend of more rapid disease progression in patients with higher AR/PSA ratios in the AMACR- areas. This study demonstrates the feasibility of developing reproducible protocols for TRF imaging and automated image analysis to study the expression of AR and PSA in benign and malignant prostate. It also highlighted the differences in AR and PSA protein expression within AMACR- and AMACR+ cancer regions.
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- author
- Krzyzanowska, Agnieszka LU ; Lippolis, Giuseppe LU ; Helczynski, Leszek LU ; Anand, Aseem LU ; Peltola, Mari ; Pettersson, Kim ; Lilja, Hans LU and Bjartell, Anders LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- androgen receptor, biomarkers, digital image analysis, prostate cancer, prostate-specific antigen, time-resolved fluorescence imaging
- in
- Journal of Histochemistry and Cytochemistry
- volume
- 64
- issue
- 5
- pages
- 12 pages
- publisher
- Histochemical Society
- external identifiers
-
- scopus:84964886289
- pmid:27026295
- wos:000375128100003
- ISSN
- 0022-1554
- DOI
- 10.1369/0022155416640466
- language
- English
- LU publication?
- yes
- id
- b17d4c05-777a-4380-a133-92192e0c719c
- date added to LUP
- 2016-06-01 11:16:19
- date last changed
- 2024-07-12 11:30:34
@article{b17d4c05-777a-4380-a133-92192e0c719c, abstract = {{<p>Androgen receptor (AR) and prostate-specific antigen (PSA) are expressed in the prostate and are involved in prostate cancer (PCa). The aim of this study was to develop reliable protocols for reproducible quantification of AR and PSA in benign and malignant prostate tissue using time-resolved fluorescence (TRF) imaging techniques. AR and PSA were detected with TRF in tissue microarrays from 91 PCa patients. p63/ alpha-methylacyl-CoA racemase (AMACR) staining on consecutive sections was used to categorize tissue areas as benign or cancerous. Automated image analysis was used to quantify staining intensity. AR intensity was significantly higher in AMACR+ and lower in AMACR- cancer areas as compared with benign epithelium. The PSA intensity was significantly lower in cancer areas, particularly in AMACR- glands. The AR/PSA ratio varied significantly in the AMACR+ tumor cells as compared with benign glands. There was a trend of more rapid disease progression in patients with higher AR/PSA ratios in the AMACR- areas. This study demonstrates the feasibility of developing reproducible protocols for TRF imaging and automated image analysis to study the expression of AR and PSA in benign and malignant prostate. It also highlighted the differences in AR and PSA protein expression within AMACR- and AMACR+ cancer regions.</p>}}, author = {{Krzyzanowska, Agnieszka and Lippolis, Giuseppe and Helczynski, Leszek and Anand, Aseem and Peltola, Mari and Pettersson, Kim and Lilja, Hans and Bjartell, Anders}}, issn = {{0022-1554}}, keywords = {{androgen receptor; biomarkers; digital image analysis; prostate cancer; prostate-specific antigen; time-resolved fluorescence imaging}}, language = {{eng}}, number = {{5}}, pages = {{311--322}}, publisher = {{Histochemical Society}}, series = {{Journal of Histochemistry and Cytochemistry}}, title = {{Quantitative Time-Resolved Fluorescence Imaging of Androgen Receptor and Prostate-Specific Antigen in Prostate Tissue Sections}}, url = {{http://dx.doi.org/10.1369/0022155416640466}}, doi = {{10.1369/0022155416640466}}, volume = {{64}}, year = {{2016}}, }