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Quantitative Time-Resolved Fluorescence Imaging of Androgen Receptor and Prostate-Specific Antigen in Prostate Tissue Sections

Krzyzanowska, Agnieszka LU ; Lippolis, Giuseppe LU ; Helczynski, Leszek LU ; Anand, Aseem LU ; Peltola, Mari; Pettersson, Kim; Lilja, Hans LU and Bjartell, Anders LU (2016) In Journal of Histochemistry and Cytochemistry 64(5). p.311-322
Abstract

Androgen receptor (AR) and prostate-specific antigen (PSA) are expressed in the prostate and are involved in prostate cancer (PCa). The aim of this study was to develop reliable protocols for reproducible quantification of AR and PSA in benign and malignant prostate tissue using time-resolved fluorescence (TRF) imaging techniques. AR and PSA were detected with TRF in tissue microarrays from 91 PCa patients. p63/ alpha-methylacyl-CoA racemase (AMACR) staining on consecutive sections was used to categorize tissue areas as benign or cancerous. Automated image analysis was used to quantify staining intensity. AR intensity was significantly higher in AMACR+ and lower in AMACR- cancer areas as compared with benign epithelium. The PSA... (More)

Androgen receptor (AR) and prostate-specific antigen (PSA) are expressed in the prostate and are involved in prostate cancer (PCa). The aim of this study was to develop reliable protocols for reproducible quantification of AR and PSA in benign and malignant prostate tissue using time-resolved fluorescence (TRF) imaging techniques. AR and PSA were detected with TRF in tissue microarrays from 91 PCa patients. p63/ alpha-methylacyl-CoA racemase (AMACR) staining on consecutive sections was used to categorize tissue areas as benign or cancerous. Automated image analysis was used to quantify staining intensity. AR intensity was significantly higher in AMACR+ and lower in AMACR- cancer areas as compared with benign epithelium. The PSA intensity was significantly lower in cancer areas, particularly in AMACR- glands. The AR/PSA ratio varied significantly in the AMACR+ tumor cells as compared with benign glands. There was a trend of more rapid disease progression in patients with higher AR/PSA ratios in the AMACR- areas. This study demonstrates the feasibility of developing reproducible protocols for TRF imaging and automated image analysis to study the expression of AR and PSA in benign and malignant prostate. It also highlighted the differences in AR and PSA protein expression within AMACR- and AMACR+ cancer regions.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
androgen receptor, biomarkers, digital image analysis, prostate cancer, prostate-specific antigen, time-resolved fluorescence imaging
in
Journal of Histochemistry and Cytochemistry
volume
64
issue
5
pages
12 pages
publisher
Histochemical Society
external identifiers
  • Scopus:84964886289
  • WOS:000375128100003
ISSN
0022-1554
DOI
10.1369/0022155416640466
language
English
LU publication?
yes
id
b17d4c05-777a-4380-a133-92192e0c719c
date added to LUP
2016-06-01 11:16:19
date last changed
2017-02-22 11:44:45
@article{b17d4c05-777a-4380-a133-92192e0c719c,
  abstract     = {<p>Androgen receptor (AR) and prostate-specific antigen (PSA) are expressed in the prostate and are involved in prostate cancer (PCa). The aim of this study was to develop reliable protocols for reproducible quantification of AR and PSA in benign and malignant prostate tissue using time-resolved fluorescence (TRF) imaging techniques. AR and PSA were detected with TRF in tissue microarrays from 91 PCa patients. p63/ alpha-methylacyl-CoA racemase (AMACR) staining on consecutive sections was used to categorize tissue areas as benign or cancerous. Automated image analysis was used to quantify staining intensity. AR intensity was significantly higher in AMACR+ and lower in AMACR- cancer areas as compared with benign epithelium. The PSA intensity was significantly lower in cancer areas, particularly in AMACR- glands. The AR/PSA ratio varied significantly in the AMACR+ tumor cells as compared with benign glands. There was a trend of more rapid disease progression in patients with higher AR/PSA ratios in the AMACR- areas. This study demonstrates the feasibility of developing reproducible protocols for TRF imaging and automated image analysis to study the expression of AR and PSA in benign and malignant prostate. It also highlighted the differences in AR and PSA protein expression within AMACR- and AMACR+ cancer regions.</p>},
  author       = {Krzyzanowska, Agnieszka and Lippolis, Giuseppe and Helczynski, Leszek and Anand, Aseem and Peltola, Mari and Pettersson, Kim and Lilja, Hans and Bjartell, Anders},
  issn         = {0022-1554},
  keyword      = {androgen receptor,biomarkers,digital image analysis,prostate cancer,prostate-specific antigen,time-resolved fluorescence imaging},
  language     = {eng},
  number       = {5},
  pages        = {311--322},
  publisher    = {Histochemical Society},
  series       = {Journal of Histochemistry and Cytochemistry},
  title        = {Quantitative Time-Resolved Fluorescence Imaging of Androgen Receptor and Prostate-Specific Antigen in Prostate Tissue Sections},
  url          = {http://dx.doi.org/10.1369/0022155416640466},
  volume       = {64},
  year         = {2016},
}