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Non-invasive monitoring of protein adsorption and removal in a turbulent flow cell

Karlsson, Camilla A.C. LU ; Wahlgren, Marie C. LU orcid and Trägårdh, A. Christian LU (2001) In Colloids and Surfaces B: Biointerfaces 20(1). p.9-25
Abstract

A flow cell was designed for continuous non-invasive measurements of macromolecular adsorption and removal under well-defined flow conditions. The adsorption of the protein β-lactoglobulin to stainless steel surfaces and its subsequent detergent-induced removal were followed in the flow cell using in situ ellipsometry under both laminar and turbulent conditions. Factors varied include the cleaning agent type and concentration, the flow rate and the protein concentration. In addition, the experiments studying different cleaning agents were repeated in a stirred cuvette system employed in previous work. The ellipsometer as well as the experimental set-ups are described, with particular emphasis on the flow cell design. Evaluation of the... (More)

A flow cell was designed for continuous non-invasive measurements of macromolecular adsorption and removal under well-defined flow conditions. The adsorption of the protein β-lactoglobulin to stainless steel surfaces and its subsequent detergent-induced removal were followed in the flow cell using in situ ellipsometry under both laminar and turbulent conditions. Factors varied include the cleaning agent type and concentration, the flow rate and the protein concentration. In addition, the experiments studying different cleaning agents were repeated in a stirred cuvette system employed in previous work. The ellipsometer as well as the experimental set-ups are described, with particular emphasis on the flow cell design. Evaluation of the results is done by comparing the amounts of protein adsorbed from solution, the initial adsorption rates, the amounts removed in water or detergent and the residual amounts after cleaning. The results are discussed in terms of β-lactoglobulin unfolding, preferential adsorption, different states of adsorbed molecules, additional physicochemical aspects and hydrodynamic characteristics. (C) 2001 Elsevier Science B.V.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
β-Lactoglobulin, Adsorption, Cleaning, Ellipsometry, Laminar and turbulent flow
in
Colloids and Surfaces B: Biointerfaces
volume
20
issue
1
pages
17 pages
publisher
Elsevier
external identifiers
  • scopus:0035864029
ISSN
0927-7765
DOI
10.1016/S0927-7765(99)00134-4
language
English
LU publication?
yes
id
b1f56cab-79a0-4b1c-87a9-86c197309500
date added to LUP
2018-12-16 10:33:41
date last changed
2021-02-17 02:00:37
@article{b1f56cab-79a0-4b1c-87a9-86c197309500,
  abstract     = {<p>A flow cell was designed for continuous non-invasive measurements of macromolecular adsorption and removal under well-defined flow conditions. The adsorption of the protein β-lactoglobulin to stainless steel surfaces and its subsequent detergent-induced removal were followed in the flow cell using in situ ellipsometry under both laminar and turbulent conditions. Factors varied include the cleaning agent type and concentration, the flow rate and the protein concentration. In addition, the experiments studying different cleaning agents were repeated in a stirred cuvette system employed in previous work. The ellipsometer as well as the experimental set-ups are described, with particular emphasis on the flow cell design. Evaluation of the results is done by comparing the amounts of protein adsorbed from solution, the initial adsorption rates, the amounts removed in water or detergent and the residual amounts after cleaning. The results are discussed in terms of β-lactoglobulin unfolding, preferential adsorption, different states of adsorbed molecules, additional physicochemical aspects and hydrodynamic characteristics. (C) 2001 Elsevier Science B.V.</p>},
  author       = {Karlsson, Camilla A.C. and Wahlgren, Marie C. and Trägårdh, A. Christian},
  issn         = {0927-7765},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {9--25},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Non-invasive monitoring of protein adsorption and removal in a turbulent flow cell},
  url          = {http://dx.doi.org/10.1016/S0927-7765(99)00134-4},
  doi          = {10.1016/S0927-7765(99)00134-4},
  volume       = {20},
  year         = {2001},
}