Non-invasive monitoring of protein adsorption and removal in a turbulent flow cell
Karlsson, Camilla A.C. LU ; Wahlgren, Marie C. LU
and Trägårdh, A. Christian
LU
(2001)
In Colloids and Surfaces B: Biointerfaces
20(1).
p.9-25
- Abstract
A flow cell was designed for continuous non-invasive measurements of macromolecular adsorption and removal under well-defined flow conditions. The adsorption of the protein β-lactoglobulin to stainless steel surfaces and its subsequent detergent-induced removal were followed in the flow cell using in situ ellipsometry under both laminar and turbulent conditions. Factors varied include the cleaning agent type and concentration, the flow rate and the protein concentration. In addition, the experiments studying different cleaning agents were repeated in a stirred cuvette system employed in previous work. The ellipsometer as well as the experimental set-ups are described, with particular emphasis on the flow cell design. Evaluation of the... (More)
A flow cell was designed for continuous non-invasive measurements of macromolecular adsorption and removal under well-defined flow conditions. The adsorption of the protein β-lactoglobulin to stainless steel surfaces and its subsequent detergent-induced removal were followed in the flow cell using in situ ellipsometry under both laminar and turbulent conditions. Factors varied include the cleaning agent type and concentration, the flow rate and the protein concentration. In addition, the experiments studying different cleaning agents were repeated in a stirred cuvette system employed in previous work. The ellipsometer as well as the experimental set-ups are described, with particular emphasis on the flow cell design. Evaluation of the results is done by comparing the amounts of protein adsorbed from solution, the initial adsorption rates, the amounts removed in water or detergent and the residual amounts after cleaning. The results are discussed in terms of β-lactoglobulin unfolding, preferential adsorption, different states of adsorbed molecules, additional physicochemical aspects and hydrodynamic characteristics. (C) 2001 Elsevier Science B.V.
(Less)
- author
- Karlsson, Camilla A.C.
LU
; Wahlgren, Marie C.
LU
and Trägårdh, A. Christian
LU
- organization
- publishing date
- 2001-01-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- β-Lactoglobulin, Adsorption, Cleaning, Ellipsometry, Laminar and turbulent flow
- in
- Colloids and Surfaces B: Biointerfaces
- volume
- 20
- issue
- 1
- pages
- 17 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0035864029
- ISSN
- 0927-7765
- DOI
- 10.1016/S0927-7765(99)00134-4
- language
- English
- LU publication?
- yes
- id
- b1f56cab-79a0-4b1c-87a9-86c197309500
- date added to LUP
- 2018-12-16 10:33:41
- date last changed
- 2023-09-08 14:07:12
@article{b1f56cab-79a0-4b1c-87a9-86c197309500,
abstract = {{<p>A flow cell was designed for continuous non-invasive measurements of macromolecular adsorption and removal under well-defined flow conditions. The adsorption of the protein β-lactoglobulin to stainless steel surfaces and its subsequent detergent-induced removal were followed in the flow cell using in situ ellipsometry under both laminar and turbulent conditions. Factors varied include the cleaning agent type and concentration, the flow rate and the protein concentration. In addition, the experiments studying different cleaning agents were repeated in a stirred cuvette system employed in previous work. The ellipsometer as well as the experimental set-ups are described, with particular emphasis on the flow cell design. Evaluation of the results is done by comparing the amounts of protein adsorbed from solution, the initial adsorption rates, the amounts removed in water or detergent and the residual amounts after cleaning. The results are discussed in terms of β-lactoglobulin unfolding, preferential adsorption, different states of adsorbed molecules, additional physicochemical aspects and hydrodynamic characteristics. (C) 2001 Elsevier Science B.V.</p>}},
author = {{Karlsson, Camilla A.C. and Wahlgren, Marie C. and Trägårdh, A. Christian}},
issn = {{0927-7765}},
keywords = {{β-Lactoglobulin; Adsorption; Cleaning; Ellipsometry; Laminar and turbulent flow}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{9--25}},
publisher = {{Elsevier}},
series = {{Colloids and Surfaces B: Biointerfaces}},
title = {{Non-invasive monitoring of protein adsorption and removal in a turbulent flow cell}},
url = {{http://dx.doi.org/10.1016/S0927-7765(99)00134-4}},
doi = {{10.1016/S0927-7765(99)00134-4}},
volume = {{20}},
year = {{2001}},
}