Size-selectivity of the glomerular barrier to high molecular weight proteins: upper size limitations of shunt pathways
(1998) In Kidney International 53(3). p.709-715- Abstract
- To evaluate the large pore radius of the glomerular capillary filter, plasma-to-urine fractional clearances of a number of endogenous proteins were assessed in normal and in nephrotic Wistar rats in which proximal tubular reabsorption had been inhibited using lysine. The proteins studied varied in radius from 16.2 A (Beta 2-microglobulin) to 90 A (alpha 2-macroglobulin). The nephrotic syndrome was induced by puromycin aminonucleoside (PAN). A marked restriction of the transport of large proteins across the glomerular capillary wall was found, indicating that there are no non-discriminatory 'shunt pathways' in the glomerular barrier. Rather, there seems to be large pores of radius 110 to 115 A accounting for the clearance of large proteins... (More)
- To evaluate the large pore radius of the glomerular capillary filter, plasma-to-urine fractional clearances of a number of endogenous proteins were assessed in normal and in nephrotic Wistar rats in which proximal tubular reabsorption had been inhibited using lysine. The proteins studied varied in radius from 16.2 A (Beta 2-microglobulin) to 90 A (alpha 2-macroglobulin). The nephrotic syndrome was induced by puromycin aminonucleoside (PAN). A marked restriction of the transport of large proteins across the glomerular capillary wall was found, indicating that there are no non-discriminatory 'shunt pathways' in the glomerular barrier. Rather, there seems to be large pores of radius 110 to 115 A accounting for the clearance of large proteins into the primary urine. This protein excretion pattern was almost the same for control and nephrotic rats, except that in the latter, the number of large pores was increased 170 times. The ratio between the number of large and small pores was calculated to be approximately equal to 7 x 10(-7) in normal rats and to 1.2 x 10(-4) in PAN nephrotic rats, assuming no classic shunt pathways. If classic shunt pathways had still existed, they would normally contribute to no more than approximately equal to 10(-5) of the total glomerular filtration rate. We postulate that very large macromolecules like IgM will not pass the glomerular filter at all under normal conditions, whereas the urine concentration of alpha2-macroglobulin will normally be extremely low. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1113002
- author
- Tencer, Jan LU ; Frick, Inga-Maria LU ; Öquist, Björn W ; Alm, Per LU and Rippe, Bengt LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glomerular capillary filter, size selectivity of glomerular barrier, proteins, shunt pathways, clearance
- in
- Kidney International
- volume
- 53
- issue
- 3
- pages
- 709 - 715
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:9507218
- scopus:0031939927
- pmid:9507218
- ISSN
- 1523-1755
- DOI
- 10.1046/j.1523-1755.1998.00797.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Nephrology (013230024), Pathology, (Lund) (013030000), Division of Infection Medicine (BMC) (013024020)
- id
- b43a7e8b-58b6-4574-8f12-42598a82a8a7 (old id 1113002)
- date added to LUP
- 2016-04-01 16:33:25
- date last changed
- 2022-03-22 19:26:11
@article{b43a7e8b-58b6-4574-8f12-42598a82a8a7,
abstract = {{To evaluate the large pore radius of the glomerular capillary filter, plasma-to-urine fractional clearances of a number of endogenous proteins were assessed in normal and in nephrotic Wistar rats in which proximal tubular reabsorption had been inhibited using lysine. The proteins studied varied in radius from 16.2 A (Beta 2-microglobulin) to 90 A (alpha 2-macroglobulin). The nephrotic syndrome was induced by puromycin aminonucleoside (PAN). A marked restriction of the transport of large proteins across the glomerular capillary wall was found, indicating that there are no non-discriminatory 'shunt pathways' in the glomerular barrier. Rather, there seems to be large pores of radius 110 to 115 A accounting for the clearance of large proteins into the primary urine. This protein excretion pattern was almost the same for control and nephrotic rats, except that in the latter, the number of large pores was increased 170 times. The ratio between the number of large and small pores was calculated to be approximately equal to 7 x 10(-7) in normal rats and to 1.2 x 10(-4) in PAN nephrotic rats, assuming no classic shunt pathways. If classic shunt pathways had still existed, they would normally contribute to no more than approximately equal to 10(-5) of the total glomerular filtration rate. We postulate that very large macromolecules like IgM will not pass the glomerular filter at all under normal conditions, whereas the urine concentration of alpha2-macroglobulin will normally be extremely low.}},
author = {{Tencer, Jan and Frick, Inga-Maria and Öquist, Björn W and Alm, Per and Rippe, Bengt}},
issn = {{1523-1755}},
keywords = {{glomerular capillary filter; size selectivity of glomerular barrier; proteins; shunt pathways; clearance}},
language = {{eng}},
number = {{3}},
pages = {{709--715}},
publisher = {{Nature Publishing Group}},
series = {{Kidney International}},
title = {{Size-selectivity of the glomerular barrier to high molecular weight proteins: upper size limitations of shunt pathways}},
url = {{http://dx.doi.org/10.1046/j.1523-1755.1998.00797.x}},
doi = {{10.1046/j.1523-1755.1998.00797.x}},
volume = {{53}},
year = {{1998}},
}