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Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans

Persson, Andrea ; Tykesson, Emil LU orcid ; Ellervik, Ulf LU and Mani, Katrin LU (2022) In Methods in Molecular Biology 2303. p.173-182
Abstract

Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with... (More)

Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with 2-aminoacridone, and quantification using high-pressure liquid chromatography (HPLC). Here, we describe the procedure of producing xyloside-primed GAGs and how to study them structurally by disaccharide analysis.

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Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Chondroitin sulfate, Chondroitinase, Dermatan sulfate, Glycosaminoglycan, Heparan sulfate, Heparinase, High-pressure liquid chromatography (HPLC), Proteoglycan, Structural analysis, Xyloside
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
2303
pages
10 pages
publisher
Humana Press
external identifiers
  • scopus:85117104221
  • pmid:34626379
ISSN
1940-6029
1064-3745
DOI
10.1007/978-1-0716-1398-6_15
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
id
b73947bb-ba9c-4169-84b3-633297ff37d2
date added to LUP
2021-11-01 13:49:53
date last changed
2022-07-28 04:47:47
@inbook{b73947bb-ba9c-4169-84b3-633297ff37d2,
  abstract     = {{<p>Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with 2-aminoacridone, and quantification using high-pressure liquid chromatography (HPLC). Here, we describe the procedure of producing xyloside-primed GAGs and how to study them structurally by disaccharide analysis.</p>}},
  author       = {{Persson, Andrea and Tykesson, Emil and Ellervik, Ulf and Mani, Katrin}},
  booktitle    = {{Methods in Molecular Biology}},
  issn         = {{1940-6029}},
  keywords     = {{Chondroitin sulfate; Chondroitinase; Dermatan sulfate; Glycosaminoglycan; Heparan sulfate; Heparinase; High-pressure liquid chromatography (HPLC); Proteoglycan; Structural analysis; Xyloside}},
  language     = {{eng}},
  pages        = {{173--182}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-1398-6_15}},
  doi          = {{10.1007/978-1-0716-1398-6_15}},
  volume       = {{2303}},
  year         = {{2022}},
}