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Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals

Friis-Hansen, L ; Wierup, Nils LU ; Rehfeld, J F and Sundler, Frank LU (2005) In Cell and Tissue Research 321(1). p.141-146
Abstract
The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of... (More)
The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of both mouse models compared with wildtypes. Thus, gastrin is not necessary for the formation of G-cells as such but the lack of gastrin reduces the number and size of their secretory granules suggesting that gastrin is vital for the formation and/or maintenance of secretory granules in G-cells. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
transgenic mice, ultrastructure, G-cells, secretion, gastrin, cholecystokinin
in
Cell and Tissue Research
volume
321
issue
1
pages
141 - 146
publisher
Springer
external identifiers
  • pmid:15906100
  • wos:000230317000015
  • scopus:22144441290
ISSN
1432-0878
DOI
10.1007/s00441-005-1110-z
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008)
id
b7dc7a09-1753-4b74-94cb-510a300d2b95 (old id 233540)
date added to LUP
2016-04-01 11:55:29
date last changed
2025-10-14 11:47:16
@article{b7dc7a09-1753-4b74-94cb-510a300d2b95,
  abstract     = {{The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of both mouse models compared with wildtypes. Thus, gastrin is not necessary for the formation of G-cells as such but the lack of gastrin reduces the number and size of their secretory granules suggesting that gastrin is vital for the formation and/or maintenance of secretory granules in G-cells.}},
  author       = {{Friis-Hansen, L and Wierup, Nils and Rehfeld, J F and Sundler, Frank}},
  issn         = {{1432-0878}},
  keywords     = {{transgenic mice; ultrastructure; G-cells; secretion; gastrin; cholecystokinin}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{141--146}},
  publisher    = {{Springer}},
  series       = {{Cell and Tissue Research}},
  title        = {{Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals}},
  url          = {{http://dx.doi.org/10.1007/s00441-005-1110-z}},
  doi          = {{10.1007/s00441-005-1110-z}},
  volume       = {{321}},
  year         = {{2005}},
}