Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals
(2005) In Cell and Tissue Research 321(1). p.141-146- Abstract
- The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of... (More)
- The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of both mouse models compared with wildtypes. Thus, gastrin is not necessary for the formation of G-cells as such but the lack of gastrin reduces the number and size of their secretory granules suggesting that gastrin is vital for the formation and/or maintenance of secretory granules in G-cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/233540
- author
- Friis-Hansen, L ; Wierup, Nils LU ; Rehfeld, J F and Sundler, Frank LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- transgenic mice, ultrastructure, G-cells, secretion, gastrin, cholecystokinin
- in
- Cell and Tissue Research
- volume
- 321
- issue
- 1
- pages
- 141 - 146
- publisher
- Springer
- external identifiers
-
- pmid:15906100
- wos:000230317000015
- scopus:22144441290
- ISSN
- 1432-0878
- DOI
- 10.1007/s00441-005-1110-z
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008)
- id
- b7dc7a09-1753-4b74-94cb-510a300d2b95 (old id 233540)
- date added to LUP
- 2016-04-01 11:55:29
- date last changed
- 2022-01-26 20:16:08
@article{b7dc7a09-1753-4b74-94cb-510a300d2b95, abstract = {{The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of both mouse models compared with wildtypes. Thus, gastrin is not necessary for the formation of G-cells as such but the lack of gastrin reduces the number and size of their secretory granules suggesting that gastrin is vital for the formation and/or maintenance of secretory granules in G-cells.}}, author = {{Friis-Hansen, L and Wierup, Nils and Rehfeld, J F and Sundler, Frank}}, issn = {{1432-0878}}, keywords = {{transgenic mice; ultrastructure; G-cells; secretion; gastrin; cholecystokinin}}, language = {{eng}}, number = {{1}}, pages = {{141--146}}, publisher = {{Springer}}, series = {{Cell and Tissue Research}}, title = {{Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals}}, url = {{http://dx.doi.org/10.1007/s00441-005-1110-z}}, doi = {{10.1007/s00441-005-1110-z}}, volume = {{321}}, year = {{2005}}, }