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Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform

Syamila, Noor ; Hamzah, Amir Syahir Amir ; Laurell, Thomas LU ; Sulaiman, Yusran ; Ikeno, Shinya ; Siang Tan, Wen and Ahmad Tajudin, Asilah LU (2024) In Journal of Micromechanics and Microengineering 34(1).
Abstract

The displacement of an electroactive monitoring agent, i.e., polyamidoamine dendrimers encapsulated gold nanoparticles (PAMAM-Au) upon the presence of a target antibody via acoustic streaming has been studied. Acoustic streaming has been used to improve the mass transfer and reduce the sample incubation rate, thus this study investigated its ability in enhancing the PAMAM-Au displacement efficiency of our immunosensor. For this purpose, the bio-nanogate components of maltose-binding protein carrying the antigenic determinant (MBP-aD) of hepatitis B surface antigen (HBsAg) as a bioreceptor was functionalized, followed by the monitoring agent i.e. PAMAM-Au on the electrode prior to the incubation with targeted anti-hepatitis B surface... (More)

The displacement of an electroactive monitoring agent, i.e., polyamidoamine dendrimers encapsulated gold nanoparticles (PAMAM-Au) upon the presence of a target antibody via acoustic streaming has been studied. Acoustic streaming has been used to improve the mass transfer and reduce the sample incubation rate, thus this study investigated its ability in enhancing the PAMAM-Au displacement efficiency of our immunosensor. For this purpose, the bio-nanogate components of maltose-binding protein carrying the antigenic determinant (MBP-aD) of hepatitis B surface antigen (HBsAg) as a bioreceptor was functionalized, followed by the monitoring agent i.e. PAMAM-Au on the electrode prior to the incubation with targeted anti-hepatitis B surface antigen (anti-HBsAg) antibody. The modified electrode was then coupled with a piezotransducer and connected to the signal transducer to induce acoustic streaming upon sample incubation. Under optimal acoustic actuation, the sample incubation time has been reduced from 20 min to 8 min via the enhancement of PAMAM-Au displacement induced by acoustic streaming. The result also demonstrated that the specificity and selectivity of the sensing platform under acoustic actuation are comparable to the static incubation in detecting the targeted antibody.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acoustic streaming, anti-HBsAg antibody, bio-nanogate, displacement-based immunosensor, PAMAM-Au
in
Journal of Micromechanics and Microengineering
volume
34
issue
1
article number
015001
publisher
IOP Publishing
external identifiers
  • scopus:85178137974
ISSN
0960-1317
DOI
10.1088/1361-6439/ad0e42
language
English
LU publication?
yes
id
b8344303-2140-4b5f-9d20-5d071b87abf4
date added to LUP
2023-12-18 15:09:05
date last changed
2025-04-04 15:02:35
@article{b8344303-2140-4b5f-9d20-5d071b87abf4,
  abstract     = {{<p>The displacement of an electroactive monitoring agent, i.e., polyamidoamine dendrimers encapsulated gold nanoparticles (PAMAM-Au) upon the presence of a target antibody via acoustic streaming has been studied. Acoustic streaming has been used to improve the mass transfer and reduce the sample incubation rate, thus this study investigated its ability in enhancing the PAMAM-Au displacement efficiency of our immunosensor. For this purpose, the bio-nanogate components of maltose-binding protein carrying the antigenic determinant (MBP-aD) of hepatitis B surface antigen (HBsAg) as a bioreceptor was functionalized, followed by the monitoring agent i.e. PAMAM-Au on the electrode prior to the incubation with targeted anti-hepatitis B surface antigen (anti-HBsAg) antibody. The modified electrode was then coupled with a piezotransducer and connected to the signal transducer to induce acoustic streaming upon sample incubation. Under optimal acoustic actuation, the sample incubation time has been reduced from 20 min to 8 min via the enhancement of PAMAM-Au displacement induced by acoustic streaming. The result also demonstrated that the specificity and selectivity of the sensing platform under acoustic actuation are comparable to the static incubation in detecting the targeted antibody.</p>}},
  author       = {{Syamila, Noor and Hamzah, Amir Syahir Amir and Laurell, Thomas and Sulaiman, Yusran and Ikeno, Shinya and Siang Tan, Wen and Ahmad Tajudin, Asilah}},
  issn         = {{0960-1317}},
  keywords     = {{acoustic streaming; anti-HBsAg antibody; bio-nanogate; displacement-based immunosensor; PAMAM-Au}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{IOP Publishing}},
  series       = {{Journal of Micromechanics and Microengineering}},
  title        = {{Displacement of PAMAM-Au via acoustic streaming on an electrochemical immunosensing platform}},
  url          = {{http://dx.doi.org/10.1088/1361-6439/ad0e42}},
  doi          = {{10.1088/1361-6439/ad0e42}},
  volume       = {{34}},
  year         = {{2024}},
}