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The heme and radical scavenger α1-microglobulin (A1M) confers early protection of the immature brain following preterm intraventricular hemorrhage

Romantsik, Olga LU ; Agyemang, Alex Adusei LU ; Sveinsdóttir, Snjolaug LU ; Rutardóttir, Sigurbjörg LU ; Holmqvist, Bo LU ; Cinthio, Magnus LU ; Mörgelin, Mattias LU ; Gumus, Gulcin ; Karlsson, Helena LU and Hansson, Stefan R. LU , et al. (2019) In Journal of Neuroinflammation 16(1).
Abstract

Background: Germinal matrix intraventricular hemorrhage (GM-IVH) is associated with cerebro-cerebellar damage in very preterm infants, leading to neurodevelopmental impairment. Penetration, from the intraventricular space, of extravasated red blood cells and extracellular hemoglobin (Hb), to the periventricular parenchyma and the cerebellum has been shown to be causal in the development of brain injury following GM-IVH. Furthermore, the damage has been described to be associated with the cytotoxic nature of extracellular Hb-metabolites. To date, there is no therapy available to prevent infants from developing either hydrocephalus or serious neurological disability. Mechanisms previously described to cause brain damage following GM-IVH,... (More)

Background: Germinal matrix intraventricular hemorrhage (GM-IVH) is associated with cerebro-cerebellar damage in very preterm infants, leading to neurodevelopmental impairment. Penetration, from the intraventricular space, of extravasated red blood cells and extracellular hemoglobin (Hb), to the periventricular parenchyma and the cerebellum has been shown to be causal in the development of brain injury following GM-IVH. Furthermore, the damage has been described to be associated with the cytotoxic nature of extracellular Hb-metabolites. To date, there is no therapy available to prevent infants from developing either hydrocephalus or serious neurological disability. Mechanisms previously described to cause brain damage following GM-IVH, i.e., oxidative stress and Hb-metabolite toxicity, suggest that the free radical and heme scavenger α1-microglobulin (A1M) may constitute a potential neuroprotective intervention. Methods: Using a preterm rabbit pup model of IVH, where IVH was induced shortly after birth in pups delivered by cesarean section at E29 (3 days prior to term), we investigated the brain distribution of recombinant A1M (rA1M) following intracerebroventricular (i.c.v.) administration at 24 h post-IVH induction. Further, short-term functional protection of i.c.v.-administered human A1M (hA1M) following IVH in the preterm rabbit pup model was evaluated. Results: Following i.c.v. administration, rA1M was distributed in periventricular white matter regions, throughout the fore- and midbrain and extending to the cerebellum. The regional distribution of rA1M was accompanied by a high co-existence of positive staining for extracellular Hb. Administration of i.c.v.-injected hA1M was associated with decreased structural tissue and mitochondrial damage and with reduced mRNA expression for proinflammatory and inflammatory signaling-related genes induced by IVH in periventricular brain tissue. Conclusions: The results of this study indicate that rA1M/hA1M is a potential candidate for neuroprotective treatment following preterm IVH.

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type
Contribution to journal
publication status
published
subject
keywords
Damage to the immature brain, Hemoglobin, Intraventricular hemorrhage, Oxidative stress, α-microglobulin
in
Journal of Neuroinflammation
volume
16
issue
1
article number
122
publisher
BioMed Central (BMC)
external identifiers
  • scopus:85066875595
  • pmid:31174551
ISSN
1742-2094
DOI
10.1186/s12974-019-1486-4
language
English
LU publication?
yes
id
b8a2fb87-e10d-4ccc-b14e-aac5dfdd9b49
alternative location
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6554963/
date added to LUP
2019-06-27 12:05:11
date last changed
2021-02-23 04:02:34
@article{b8a2fb87-e10d-4ccc-b14e-aac5dfdd9b49,
  abstract     = {<p>Background: Germinal matrix intraventricular hemorrhage (GM-IVH) is associated with cerebro-cerebellar damage in very preterm infants, leading to neurodevelopmental impairment. Penetration, from the intraventricular space, of extravasated red blood cells and extracellular hemoglobin (Hb), to the periventricular parenchyma and the cerebellum has been shown to be causal in the development of brain injury following GM-IVH. Furthermore, the damage has been described to be associated with the cytotoxic nature of extracellular Hb-metabolites. To date, there is no therapy available to prevent infants from developing either hydrocephalus or serious neurological disability. Mechanisms previously described to cause brain damage following GM-IVH, i.e., oxidative stress and Hb-metabolite toxicity, suggest that the free radical and heme scavenger α<sub>1</sub>-microglobulin (A1M) may constitute a potential neuroprotective intervention. Methods: Using a preterm rabbit pup model of IVH, where IVH was induced shortly after birth in pups delivered by cesarean section at E29 (3 days prior to term), we investigated the brain distribution of recombinant A1M (rA1M) following intracerebroventricular (i.c.v.) administration at 24 h post-IVH induction. Further, short-term functional protection of i.c.v.-administered human A1M (hA1M) following IVH in the preterm rabbit pup model was evaluated. Results: Following i.c.v. administration, rA1M was distributed in periventricular white matter regions, throughout the fore- and midbrain and extending to the cerebellum. The regional distribution of rA1M was accompanied by a high co-existence of positive staining for extracellular Hb. Administration of i.c.v.-injected hA1M was associated with decreased structural tissue and mitochondrial damage and with reduced mRNA expression for proinflammatory and inflammatory signaling-related genes induced by IVH in periventricular brain tissue. Conclusions: The results of this study indicate that rA1M/hA1M is a potential candidate for neuroprotective treatment following preterm IVH.</p>},
  author       = {Romantsik, Olga and Agyemang, Alex Adusei and Sveinsdóttir, Snjolaug and Rutardóttir, Sigurbjörg and Holmqvist, Bo and Cinthio, Magnus and Mörgelin, Mattias and Gumus, Gulcin and Karlsson, Helena and Hansson, Stefan R. and Åkerström, Bo and Ley, David and Gram, Magnus},
  issn         = {1742-2094},
  language     = {eng},
  month        = {06},
  number       = {1},
  publisher    = {BioMed Central (BMC)},
  series       = {Journal of Neuroinflammation},
  title        = {The heme and radical scavenger α<sub>1</sub>-microglobulin (A1M) confers early protection of the immature brain following preterm intraventricular hemorrhage},
  url          = {http://dx.doi.org/10.1186/s12974-019-1486-4},
  doi          = {10.1186/s12974-019-1486-4},
  volume       = {16},
  year         = {2019},
}