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Use of dielectrophoresis for directing T cells to microwells before nanostraw transfection : modelling and experiments

Lard, Mercy LU ; Ho, Bao D. LU ; Beech, Jason P. LU ; Tegenfeldt, Jonas O. LU orcid and Prinz, Christelle N. LU (2022) In RSC Advances 12(47). p.30295-30303
Abstract

Nanostraw substrates have great potential for achieving minimally invasive cell transfection. Cells located on the nanostraw substrate are subjected to mild DC electric pulses applied across the nanostraw substrate, which open pores in the cell membrane on top of the nanostraws and drives charged cargo through these pores via electrophoresis. However, with this method, the current may leak through uncovered nanostraws, thereby decreasing the desired effect in the cell-covered nanostraws. A minimization of the number of uncovered nanostraws could be achieved by high cell coverage, but this is challenging when working with small cell populations. Nanostraw substrates of smaller area could be covered by smaller cell populations but are... (More)

Nanostraw substrates have great potential for achieving minimally invasive cell transfection. Cells located on the nanostraw substrate are subjected to mild DC electric pulses applied across the nanostraw substrate, which open pores in the cell membrane on top of the nanostraws and drives charged cargo through these pores via electrophoresis. However, with this method, the current may leak through uncovered nanostraws, thereby decreasing the desired effect in the cell-covered nanostraws. A minimization of the number of uncovered nanostraws could be achieved by high cell coverage, but this is challenging when working with small cell populations. Nanostraw substrates of smaller area could be covered by smaller cell populations but are hard to integrate into fluidics systems. Here, we use simulations and experiments to show that this issue can be addressed by covering the nanostraw substrate with an insulating layer containing pores of similar size to cells. The pores act as traps into which cells can be guided using dielectrophoresis, ensuring a high degree of occupancy while maintaining a high cell viability, even if the total number of cells is low.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
RSC Advances
volume
12
issue
47
pages
9 pages
publisher
Royal Society of Chemistry
external identifiers
  • pmid:36337971
  • scopus:85142273632
ISSN
2046-2069
DOI
10.1039/d2ra05119b
language
English
LU publication?
yes
additional info
This journal is © The Royal Society of Chemistry.
id
b8d09812-99d5-40ce-b277-652fbc9c9223
date added to LUP
2022-11-10 18:24:15
date last changed
2024-06-14 23:20:20
@article{b8d09812-99d5-40ce-b277-652fbc9c9223,
  abstract     = {{<p>Nanostraw substrates have great potential for achieving minimally invasive cell transfection. Cells located on the nanostraw substrate are subjected to mild DC electric pulses applied across the nanostraw substrate, which open pores in the cell membrane on top of the nanostraws and drives charged cargo through these pores  via electrophoresis. However, with this method, the current may leak through uncovered nanostraws, thereby decreasing the desired effect in the cell-covered nanostraws. A minimization of the number of uncovered nanostraws could be achieved by high cell coverage, but this is challenging when working with small cell populations. Nanostraw substrates of smaller area could be covered by smaller cell populations but are hard to integrate into fluidics systems. Here, we use simulations and experiments to show that this issue can be addressed by covering the nanostraw substrate with an insulating layer containing pores of similar size to cells. The pores act as traps into which cells can be guided using dielectrophoresis, ensuring a high degree of occupancy while maintaining a high cell viability, even if the total number of cells is low. </p>}},
  author       = {{Lard, Mercy and Ho, Bao D. and Beech, Jason P. and Tegenfeldt, Jonas O. and Prinz, Christelle N.}},
  issn         = {{2046-2069}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{47}},
  pages        = {{30295--30303}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{RSC Advances}},
  title        = {{Use of dielectrophoresis for directing T cells to microwells before nanostraw transfection : modelling and experiments}},
  url          = {{http://dx.doi.org/10.1039/d2ra05119b}},
  doi          = {{10.1039/d2ra05119b}},
  volume       = {{12}},
  year         = {{2022}},
}