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Evidence for xylooligosaccharides utilization in Weissella strains isolated from Indian fermented foods and vegetables.

Patel, Ami LU ; Falck, Peter LU ; Shah, Nihir LU ; Immerzeel, Peter LU ; Adlercreutz, Patrick LU ; Stålbrand, Henrik LU ; Prajapati, J B; Holst, Olle LU and Nordberg Karlsson, Eva LU (2013) In FEMS Microbiology Letters 346(1). p.20-28
Abstract
Six strains isolated from fermented food were by 16S rDNA sequencing identified as Weissella species, clustering with the species-pair W. confusa/ W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS was observed for strain 85, 92, 145 and AV1, firstly by optical density measurements in microtiter plates and secondly in batch cultures also confirming concomitant decrease in pH. Analysis of XOS before and after growth established consumption in the DP2 - DP5 range in the four XOS-fermenting strains. XOS were consumed simultaneously with glucose, while xylose was consumed after glucose depletion. Cell-associated... (More)
Six strains isolated from fermented food were by 16S rDNA sequencing identified as Weissella species, clustering with the species-pair W. confusa/ W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS was observed for strain 85, 92, 145 and AV1, firstly by optical density measurements in microtiter plates and secondly in batch cultures also confirming concomitant decrease in pH. Analysis of XOS before and after growth established consumption in the DP2 - DP5 range in the four XOS-fermenting strains. XOS were consumed simultaneously with glucose, while xylose was consumed after glucose depletion. Cell-associated β-xylosidase activity was detected in the XOS fermenting strains. Analysis of genomic data suggests this activity to be linked to genes encoding glycoside hydrolases from family 3, 8 or 43. No endo-β-xylanase activity was detectable. Main fermentation end products were lactate and acetate. A higher ratio of acetic acid/lactic acid was produced during growth on XOS compared to growth on glucose. This is the first report on utilization of XOS in Weissella, indicating an increased probiotic potential for XOS-utilizing strains from the species pair W. confusa/ W. cibaria, but also showing that XOS utilization is strain-dependent for these species. This article is protected by copyright. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEMS Microbiology Letters
volume
346
issue
1
pages
20 - 28
publisher
Elsevier
external identifiers
  • wos:000322715600003
  • pmid:23738850
  • scopus:84881375389
ISSN
1574-6968
DOI
10.1111/1574-6968.12191
language
English
LU publication?
yes
id
b90d4700-2034-466b-9ef2-d4c791f83ab5 (old id 3913698)
date added to LUP
2013-07-11 13:03:38
date last changed
2019-08-23 02:22:56
@article{b90d4700-2034-466b-9ef2-d4c791f83ab5,
  abstract     = {Six strains isolated from fermented food were by 16S rDNA sequencing identified as Weissella species, clustering with the species-pair W. confusa/ W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS was observed for strain 85, 92, 145 and AV1, firstly by optical density measurements in microtiter plates and secondly in batch cultures also confirming concomitant decrease in pH. Analysis of XOS before and after growth established consumption in the DP2 - DP5 range in the four XOS-fermenting strains. XOS were consumed simultaneously with glucose, while xylose was consumed after glucose depletion. Cell-associated β-xylosidase activity was detected in the XOS fermenting strains. Analysis of genomic data suggests this activity to be linked to genes encoding glycoside hydrolases from family 3, 8 or 43. No endo-β-xylanase activity was detectable. Main fermentation end products were lactate and acetate. A higher ratio of acetic acid/lactic acid was produced during growth on XOS compared to growth on glucose. This is the first report on utilization of XOS in Weissella, indicating an increased probiotic potential for XOS-utilizing strains from the species pair W. confusa/ W. cibaria, but also showing that XOS utilization is strain-dependent for these species. This article is protected by copyright. All rights reserved.},
  author       = {Patel, Ami and Falck, Peter and Shah, Nihir and Immerzeel, Peter and Adlercreutz, Patrick and Stålbrand, Henrik and Prajapati, J B and Holst, Olle and Nordberg Karlsson, Eva},
  issn         = {1574-6968},
  language     = {eng},
  number       = {1},
  pages        = {20--28},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Evidence for xylooligosaccharides utilization in Weissella strains isolated from Indian fermented foods and vegetables.},
  url          = {http://dx.doi.org/10.1111/1574-6968.12191},
  volume       = {346},
  year         = {2013},
}