Evidence for xylooligosaccharides utilization in Weissella strains isolated from Indian fermented foods and vegetables.
Patel, Ami LU ; Falck, Peter LU ; Shah, Nihir LU ; Immerzeel, Peter LU ; Adlercreutz, Patrick LU
; Stålbrand, Henrik
LU
; Prajapati, J B
; Holst, Olle
LU
and Nordberg Karlsson, Eva
LU
(2013)
In FEMS Microbiology Letters
346(1).
p.20-28
- Abstract
- Six strains isolated from fermented food were by 16S rDNA sequencing identified as Weissella species, clustering with the species-pair W. confusa/ W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS was observed for strain 85, 92, 145 and AV1, firstly by optical density measurements in microtiter plates and secondly in batch cultures also confirming concomitant decrease in pH. Analysis of XOS before and after growth established consumption in the DP2 - DP5 range in the four XOS-fermenting strains. XOS were consumed simultaneously with glucose, while xylose was consumed after glucose depletion. Cell-associated... (More)
- Six strains isolated from fermented food were by 16S rDNA sequencing identified as Weissella species, clustering with the species-pair W. confusa/ W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS was observed for strain 85, 92, 145 and AV1, firstly by optical density measurements in microtiter plates and secondly in batch cultures also confirming concomitant decrease in pH. Analysis of XOS before and after growth established consumption in the DP2 - DP5 range in the four XOS-fermenting strains. XOS were consumed simultaneously with glucose, while xylose was consumed after glucose depletion. Cell-associated β-xylosidase activity was detected in the XOS fermenting strains. Analysis of genomic data suggests this activity to be linked to genes encoding glycoside hydrolases from family 3, 8 or 43. No endo-β-xylanase activity was detectable. Main fermentation end products were lactate and acetate. A higher ratio of acetic acid/lactic acid was produced during growth on XOS compared to growth on glucose. This is the first report on utilization of XOS in Weissella, indicating an increased probiotic potential for XOS-utilizing strains from the species pair W. confusa/ W. cibaria, but also showing that XOS utilization is strain-dependent for these species. This article is protected by copyright. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3913698
- author
- Patel, Ami
LU
; Falck, Peter
LU
; Shah, Nihir
LU
; Immerzeel, Peter
LU
; Adlercreutz, Patrick
LU
; Stålbrand, Henrik
LU
; Prajapati, J B
; Holst, Olle
LU
and Nordberg Karlsson, Eva
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEMS Microbiology Letters
- volume
- 346
- issue
- 1
- pages
- 20 - 28
- publisher
- Oxford University Press
- external identifiers
-
- wos:000322715600003
- pmid:23738850
- scopus:84881375389
- pmid:23738850
- ISSN
- 1574-6968
- DOI
- 10.1111/1574-6968.12191
- project
- ANTIDIABETIC FOOD CENTRE
- language
- English
- LU publication?
- yes
- id
- b90d4700-2034-466b-9ef2-d4c791f83ab5 (old id 3913698)
- date added to LUP
- 2016-04-01 10:00:07
- date last changed
- 2022-01-25 18:49:14
@article{b90d4700-2034-466b-9ef2-d4c791f83ab5,
abstract = {{Six strains isolated from fermented food were by 16S rDNA sequencing identified as Weissella species, clustering with the species-pair W. confusa/ W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS was observed for strain 85, 92, 145 and AV1, firstly by optical density measurements in microtiter plates and secondly in batch cultures also confirming concomitant decrease in pH. Analysis of XOS before and after growth established consumption in the DP2 - DP5 range in the four XOS-fermenting strains. XOS were consumed simultaneously with glucose, while xylose was consumed after glucose depletion. Cell-associated β-xylosidase activity was detected in the XOS fermenting strains. Analysis of genomic data suggests this activity to be linked to genes encoding glycoside hydrolases from family 3, 8 or 43. No endo-β-xylanase activity was detectable. Main fermentation end products were lactate and acetate. A higher ratio of acetic acid/lactic acid was produced during growth on XOS compared to growth on glucose. This is the first report on utilization of XOS in Weissella, indicating an increased probiotic potential for XOS-utilizing strains from the species pair W. confusa/ W. cibaria, but also showing that XOS utilization is strain-dependent for these species. This article is protected by copyright. All rights reserved.}},
author = {{Patel, Ami and Falck, Peter and Shah, Nihir and Immerzeel, Peter and Adlercreutz, Patrick and Stålbrand, Henrik and Prajapati, J B and Holst, Olle and Nordberg Karlsson, Eva}},
issn = {{1574-6968}},
language = {{eng}},
number = {{1}},
pages = {{20--28}},
publisher = {{Oxford University Press}},
series = {{FEMS Microbiology Letters}},
title = {{Evidence for xylooligosaccharides utilization in Weissella strains isolated from Indian fermented foods and vegetables.}},
url = {{http://dx.doi.org/10.1111/1574-6968.12191}},
doi = {{10.1111/1574-6968.12191}},
volume = {{346}},
year = {{2013}},
}