Prevention of Adhesions by PL/PG after Adhesiolysis
(2012) In Journal of Tissue Science & Engineering 3(4).- Abstract
- Background: Previous studies of differently charged polypeptides, Poly-L-lysine (PL) and Poly-L-Glutamate (PG) have shown promising results, reducing postoperative adhesions. This study aimed to investigate the possible anti adhesion effect of those combined polypeptides, after adhesiolys. The concentration of tPA, PAI-1 and active TGFb1 in biopsies from adhesions, unharmed peritoneum before and after adhesiolysis, was also investigated.
Materials and methods: A total of 24 male rats were divided in three groups A (N=8), B (N=8) and C (N=8). All rats underwent primary adhesion creating surgery at day 0, and adhesiolysis at day 7. Adhesions were evaluated at day 7 and 14, where group B received PL/PG after surgery at day 0 and... (More) - Background: Previous studies of differently charged polypeptides, Poly-L-lysine (PL) and Poly-L-Glutamate (PG) have shown promising results, reducing postoperative adhesions. This study aimed to investigate the possible anti adhesion effect of those combined polypeptides, after adhesiolys. The concentration of tPA, PAI-1 and active TGFb1 in biopsies from adhesions, unharmed peritoneum before and after adhesiolysis, was also investigated.
Materials and methods: A total of 24 male rats were divided in three groups A (N=8), B (N=8) and C (N=8). All rats underwent primary adhesion creating surgery at day 0, and adhesiolysis at day 7. Adhesions were evaluated at day 7 and 14, where group B received PL/PG after surgery at day 0 and after adhesiolysis at day 7, and group C received PL/PG after adhesiolysis at day 7. Tissue plasminogen activator (tPA), Plasminogen activator inhibitor 1(PAI-1) and active transforming growth factor beta 1(TGF-β1) were collected from biopsies of adhesions and normal peritoneum at day 0, 7 and 14.
Results: Significant reduction of adhesions p<0.05 was seen in group B at day 7 after primary surgery, and at day 14 after adhesiolysis. Significantly p<0.05 reduction of adhesions was seen at day 14 after adhesiolysis in group C. Some variations were seen in tPA, PAI-1 and active TGFb1.
Conclusions: PL/PG may be used to prevent adhesion formation after adhesiolysis. The process of fibrinolysis and fibrosis was not affected, after PL/PG prophylaxis and adhesiolysis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4333697
- author
- Åkerberg, Daniel LU ; Bauden, Monika LU ; Isaksson, Karolin LU ; Andersson, Roland LU and Tingstedt, Bobby LU
- organization
- publishing date
- 2012
- type
- Contribution to specialist publication or newspaper
- publication status
- published
- subject
- keywords
- Abdominal adhesions, Prevention, Polypeptides, Adhesiolysis
- categories
- Popular Science
- in
- Journal of Tissue Science & Engineering
- volume
- 3
- issue
- 4
- ISSN
- 2157-7552
- DOI
- 10.4172/2157-7552.1000121
- language
- English
- LU publication?
- yes
- id
- ba539d12-87f6-4dad-ade3-e90362b579b9 (old id 4333697)
- date added to LUP
- 2016-04-04 09:11:15
- date last changed
- 2018-11-21 20:51:21
@misc{ba539d12-87f6-4dad-ade3-e90362b579b9, abstract = {{Background: Previous studies of differently charged polypeptides, Poly-L-lysine (PL) and Poly-L-Glutamate (PG) have shown promising results, reducing postoperative adhesions. This study aimed to investigate the possible anti adhesion effect of those combined polypeptides, after adhesiolys. The concentration of tPA, PAI-1 and active TGFb1 in biopsies from adhesions, unharmed peritoneum before and after adhesiolysis, was also investigated.<br/><br> Materials and methods: A total of 24 male rats were divided in three groups A (N=8), B (N=8) and C (N=8). All rats underwent primary adhesion creating surgery at day 0, and adhesiolysis at day 7. Adhesions were evaluated at day 7 and 14, where group B received PL/PG after surgery at day 0 and after adhesiolysis at day 7, and group C received PL/PG after adhesiolysis at day 7. Tissue plasminogen activator (tPA), Plasminogen activator inhibitor 1(PAI-1) and active transforming growth factor beta 1(TGF-β1) were collected from biopsies of adhesions and normal peritoneum at day 0, 7 and 14.<br/><br> Results: Significant reduction of adhesions p<0.05 was seen in group B at day 7 after primary surgery, and at day 14 after adhesiolysis. Significantly p<0.05 reduction of adhesions was seen at day 14 after adhesiolysis in group C. Some variations were seen in tPA, PAI-1 and active TGFb1.<br/><br> Conclusions: PL/PG may be used to prevent adhesion formation after adhesiolysis. The process of fibrinolysis and fibrosis was not affected, after PL/PG prophylaxis and adhesiolysis.}}, author = {{Åkerberg, Daniel and Bauden, Monika and Isaksson, Karolin and Andersson, Roland and Tingstedt, Bobby}}, issn = {{2157-7552}}, keywords = {{Abdominal adhesions; Prevention; Polypeptides; Adhesiolysis}}, language = {{eng}}, number = {{4}}, series = {{Journal of Tissue Science & Engineering}}, title = {{Prevention of Adhesions by PL/PG after Adhesiolysis}}, url = {{http://dx.doi.org/10.4172/2157-7552.1000121}}, doi = {{10.4172/2157-7552.1000121}}, volume = {{3}}, year = {{2012}}, }