Advanced

Crystal structures of human PAICS reveal substrate and product binding of an emerging cancer target

Škerlová, Jana ; Unterlass, Judith ; Göttmann, Mona ; Marttila, Petra ; Homan, Evert ; Helleday, Thomas ; Jemth, Ann-Sofie and Stenmark, Pål LU (2020) In Journal of Biological Chemistry 295(33). p.11656-11668
Abstract

The bifunctional human enzyme phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (PAICS) catalyzes two essential steps in the de novo purine biosynthesis pathway. PAICS is overexpressed in many cancers and could be a promising target for the development of cancer therapeutics. Here, using gene knockdowns and clonogenic survival and cell viability assays, we demonstrate that PAICS is required for growth and survival of prostate cancer cells. PAICS catalyzes the carboxylation of aminoimidazole ribonucleotide (AIR) and the subsequent conversion of carboxyaminoimidazole ribonucleotide (CAIR) and L-aspartate to N-succinylcarboxamide-5-aminoimidazole ribonucleotide (SAICAR). Of note, we... (More)

The bifunctional human enzyme phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (PAICS) catalyzes two essential steps in the de novo purine biosynthesis pathway. PAICS is overexpressed in many cancers and could be a promising target for the development of cancer therapeutics. Here, using gene knockdowns and clonogenic survival and cell viability assays, we demonstrate that PAICS is required for growth and survival of prostate cancer cells. PAICS catalyzes the carboxylation of aminoimidazole ribonucleotide (AIR) and the subsequent conversion of carboxyaminoimidazole ribonucleotide (CAIR) and L-aspartate to N-succinylcarboxamide-5-aminoimidazole ribonucleotide (SAICAR). Of note, we present the first structures of human octameric PAICS in complexes with native ligands. In particular, we report the structure of PAICS with CAIR bound in the active sites of both domains and SAICAR bound in one of the SAICAR synthetase domains. Moreover, we report the PAICS structure with SAICAR and an ATP analog occupying the SAICAR synthetase active site. These structures provide insight into substrate and product binding and the architecture of the active sites, disclosing important structural information for rational design of PAICS inhibitors as potential anticancer drugs.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
295
issue
33
pages
11656 - 11668
publisher
ASBMB
external identifiers
  • pmid:32571877
  • scopus:85089787270
ISSN
1083-351X
DOI
10.1074/jbc.RA120.013695
language
English
LU publication?
yes
additional info
Published under license by The American Society for Biochemistry and Molecular Biology, Inc.
id
bbd72179-f41c-43f5-ae48-292a6ed7c253
date added to LUP
2020-06-26 08:11:29
date last changed
2020-09-26 03:00:34
@article{bbd72179-f41c-43f5-ae48-292a6ed7c253,
  abstract     = {<p>The bifunctional human enzyme phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (PAICS) catalyzes two essential steps in the de novo purine biosynthesis pathway. PAICS is overexpressed in many cancers and could be a promising target for the development of cancer therapeutics. Here, using gene knockdowns and clonogenic survival and cell viability assays, we demonstrate that PAICS is required for growth and survival of prostate cancer cells. PAICS catalyzes the carboxylation of aminoimidazole ribonucleotide (AIR) and the subsequent conversion of carboxyaminoimidazole ribonucleotide (CAIR) and L-aspartate to N-succinylcarboxamide-5-aminoimidazole ribonucleotide (SAICAR). Of note, we present the first structures of human octameric PAICS in complexes with native ligands. In particular, we report the structure of PAICS with CAIR bound in the active sites of both domains and SAICAR bound in one of the SAICAR synthetase domains. Moreover, we report the PAICS structure with SAICAR and an ATP analog occupying the SAICAR synthetase active site. These structures provide insight into substrate and product binding and the architecture of the active sites, disclosing important structural information for rational design of PAICS inhibitors as potential anticancer drugs.</p>},
  author       = {Škerlová, Jana and Unterlass, Judith and Göttmann, Mona and Marttila, Petra and Homan, Evert and Helleday, Thomas and Jemth, Ann-Sofie and Stenmark, Pål},
  issn         = {1083-351X},
  language     = {eng},
  month        = {06},
  number       = {33},
  pages        = {11656--11668},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Crystal structures of human PAICS reveal substrate and product binding of an emerging cancer target},
  url          = {http://dx.doi.org/10.1074/jbc.RA120.013695},
  doi          = {10.1074/jbc.RA120.013695},
  volume       = {295},
  year         = {2020},
}