Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Amyloid-beta Oligomers in Cerebrospinal Fluid are Associated with Cognitive Decline in Patients with Alzheimer's Disease

Santos, Alexander Navarrete ; Ewers, Michael ; Minthon, Lennart LU ; Simm, Andreas ; Silber, Rolf-Edgar ; Blennow, Kaj ; Prvulovic, David ; Hansson, Oskar LU orcid and Hampel, Harald (2012) In Journal of Alzheimer's Disease 29(1). p.171-176
Abstract
Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer... (More)
Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio A beta-oligomers/A beta(42) was significantly elevated in AD subjects compared to non-AD subjects (p=0.001). Most important, there was a negative correlation between the amount of A beta-oligomers and the Mini-Mental Status Exam score (r=-0.65; p=0.013) in AD patients. The detection of A beta-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oligomers, cytometry, flow, cognitive decline, biomarkers, amyloid-beta, Alzheimer's disease
in
Journal of Alzheimer's Disease
volume
29
issue
1
pages
171 - 176
publisher
IOS Press
external identifiers
  • wos:000301139000015
ISSN
1387-2877
DOI
10.3233/JAD-2011-111361
language
English
LU publication?
yes
id
bc13f555-1628-40cc-a4bf-2efbbb1c7cdd (old id 2517551)
date added to LUP
2016-04-01 10:08:11
date last changed
2021-09-27 04:01:32
@article{bc13f555-1628-40cc-a4bf-2efbbb1c7cdd,
  abstract     = {{Oligomers of the amyloid-beta peptide (A beta) are thought to be the most toxic form of A beta and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n=14), non-demented controls (n=12), and other neurological disorders (dementia with Lewy bodies, n=2; vascular dementia, n=1; primary progressive aphasia, n = 1) were analyzed for the presence of A beta-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-beta (A beta)(42) were determined using ELISA. CSF A beta-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio A beta-oligomers/A beta(42) was significantly elevated in AD subjects compared to non-AD subjects (p=0.001). Most important, there was a negative correlation between the amount of A beta-oligomers and the Mini-Mental Status Exam score (r=-0.65; p=0.013) in AD patients. The detection of A beta-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity.}},
  author       = {{Santos, Alexander Navarrete and Ewers, Michael and Minthon, Lennart and Simm, Andreas and Silber, Rolf-Edgar and Blennow, Kaj and Prvulovic, David and Hansson, Oskar and Hampel, Harald}},
  issn         = {{1387-2877}},
  keywords     = {{oligomers; cytometry; flow; cognitive decline; biomarkers; amyloid-beta; Alzheimer's disease}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{171--176}},
  publisher    = {{IOS Press}},
  series       = {{Journal of Alzheimer's Disease}},
  title        = {{Amyloid-beta Oligomers in Cerebrospinal Fluid are Associated with Cognitive Decline in Patients with Alzheimer's Disease}},
  url          = {{http://dx.doi.org/10.3233/JAD-2011-111361}},
  doi          = {{10.3233/JAD-2011-111361}},
  volume       = {{29}},
  year         = {{2012}},
}