Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Crossed patterned structured illumination for the analysis and velocimetry of transient turbid media

Kristensson, Elias LU and Berrocal, Edouard LU (2018) In Scientific Reports 8(1).
Abstract

Imaging through turbid environments is experimentally challenging due to multiple light scattering. Structured laser illumination has proven to be effective to minimize errors arising from this phenomenon, allowing the interior of optically dense media to be observed. However, in order to preserve the image spatial resolution while suppressing the intensity contribution from multiple light scattering, the method relies on multiple acquisitions and thus sequential illumination. These requirements significantly limit the usefulness of structured illumination when imaging highly transient events. Here we present a method for achieving snapshot visualizations using structured illumination, where the spatial frequency domain is increased by... (More)

Imaging through turbid environments is experimentally challenging due to multiple light scattering. Structured laser illumination has proven to be effective to minimize errors arising from this phenomenon, allowing the interior of optically dense media to be observed. However, in order to preserve the image spatial resolution while suppressing the intensity contribution from multiple light scattering, the method relies on multiple acquisitions and thus sequential illumination. These requirements significantly limit the usefulness of structured illumination when imaging highly transient events. Here we present a method for achieving snapshot visualizations using structured illumination, where the spatial frequency domain is increased by a factor of two compared to past structured illumination snapshots. Our approach uses two crossed intensity-modulated patterns, allowing us to expand the spatial frequency response of the extracted data. The snapshot capability of this imaging approach allows tracking single particles and opens up for the extraction of velocity vectors by combining it with standard particle tracking/image velocimetry (PTV or PIV) equipment. In this paper we demonstrate the capabilities of this new method and, for the first time, use structured illumination to extract velocity vectors in 2D in a transient turbid medium, in this case an optically dense atomizing spray.

(Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
8
issue
1
article number
11751
publisher
Nature Publishing Group
external identifiers
  • pmid:30082685
  • scopus:85051280434
ISSN
2045-2322
DOI
10.1038/s41598-018-30233-y
language
English
LU publication?
yes
id
bd600535-b0a1-43a1-a42d-2c92cf8638f3
date added to LUP
2018-09-05 14:39:09
date last changed
2021-09-22 01:55:53
@article{bd600535-b0a1-43a1-a42d-2c92cf8638f3,
  abstract     = {<p>Imaging through turbid environments is experimentally challenging due to multiple light scattering. Structured laser illumination has proven to be effective to minimize errors arising from this phenomenon, allowing the interior of optically dense media to be observed. However, in order to preserve the image spatial resolution while suppressing the intensity contribution from multiple light scattering, the method relies on multiple acquisitions and thus sequential illumination. These requirements significantly limit the usefulness of structured illumination when imaging highly transient events. Here we present a method for achieving snapshot visualizations using structured illumination, where the spatial frequency domain is increased by a factor of two compared to past structured illumination snapshots. Our approach uses two crossed intensity-modulated patterns, allowing us to expand the spatial frequency response of the extracted data. The snapshot capability of this imaging approach allows tracking single particles and opens up for the extraction of velocity vectors by combining it with standard particle tracking/image velocimetry (PTV or PIV) equipment. In this paper we demonstrate the capabilities of this new method and, for the first time, use structured illumination to extract velocity vectors in 2D in a transient turbid medium, in this case an optically dense atomizing spray.</p>},
  author       = {Kristensson, Elias and Berrocal, Edouard},
  issn         = {2045-2322},
  language     = {eng},
  month        = {12},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Crossed patterned structured illumination for the analysis and velocimetry of transient turbid media},
  url          = {https://lup.lub.lu.se/search/files/85533123/s41598_018_30233_y.pdf},
  doi          = {10.1038/s41598-018-30233-y},
  volume       = {8},
  year         = {2018},
}