Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer

González-Ceballos, Lara ; Carlos Guirado-Moreno, Jose ; Utzeri, Gianluca LU orcid ; Miguel García, José ; Fernández-Muiño, Miguel A. ; Osés, Sandra M. ; Teresa Sancho, M. ; Arnaiz, Ana ; Valente, Artur J.M. and Vallejos, Saúl (2023) In Food Chemistry 405.
Abstract

Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic... (More)

Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
1,2-Dihydroxybenzene, Catalase, Catechol, Glucose oxidase, Honey, Phenylboronic acid
in
Food Chemistry
volume
405
article number
134789
publisher
Elsevier
external identifiers
  • pmid:36347201
  • scopus:85141224639
ISSN
0308-8146
DOI
10.1016/j.foodchem.2022.134789
language
English
LU publication?
no
additional info
Publisher Copyright: © 2022 The Author(s)
id
c0f60517-99c4-4392-9930-34cff4f1a9b9
date added to LUP
2024-07-22 10:49:27
date last changed
2024-08-15 10:00:43
@article{c0f60517-99c4-4392-9930-34cff4f1a9b9,
  abstract     = {{<p>Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (&gt;24 h), eco-unfriendly (&gt;6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents’ removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).</p>}},
  author       = {{González-Ceballos, Lara and Carlos Guirado-Moreno, Jose and Utzeri, Gianluca and Miguel García, José and Fernández-Muiño, Miguel A. and Osés, Sandra M. and Teresa Sancho, M. and Arnaiz, Ana and Valente, Artur J.M. and Vallejos, Saúl}},
  issn         = {{0308-8146}},
  keywords     = {{1,2-Dihydroxybenzene; Catalase; Catechol; Glucose oxidase; Honey; Phenylboronic acid}},
  language     = {{eng}},
  month        = {{03}},
  publisher    = {{Elsevier}},
  series       = {{Food Chemistry}},
  title        = {{Straightforward purification method for the determination of the activity of glucose oxidase and catalase in honey by extracting polyphenols with a film-shaped polymer}},
  url          = {{http://dx.doi.org/10.1016/j.foodchem.2022.134789}},
  doi          = {{10.1016/j.foodchem.2022.134789}},
  volume       = {{405}},
  year         = {{2023}},
}