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Protein D expression promotes the adherence and internalization of non-typeable Haemophilus influenzae into human monocytic cells

Lazou Ahrén, Irini LU ; Janson, Håkan LU ; Forsgren, Arne LU and Riesbeck, Kristian LU (2001) In Microbial Pathogenesis 31(3). p.151-158
Abstract
Protein D, having a glycerol-3-phosphodiester phosphodiesterase activity, is found at the surface of all Haemophilus influenzae strains and is a possible virulence factor. In the present study, the involvement of protein D in the entry of NTHi into human monocytic cells is reported. Primary monocytes and the monocytic cell lines U-937 and THP-1 were infected with NTHi strain 772 and the mutant 772 Delta hpd 1 (lacking the gene for protein D). NTHi 772 adhered to and entered monocytic cells up to four-fold more efficiently compared to 772 Delta hpd 1. When an Escherichia coli transformant expressing protein D was incubated with monocytic cells, the number of intracellular bacteria increased 1.6-fold compared to protein D-deficient controls.... (More)
Protein D, having a glycerol-3-phosphodiester phosphodiesterase activity, is found at the surface of all Haemophilus influenzae strains and is a possible virulence factor. In the present study, the involvement of protein D in the entry of NTHi into human monocytic cells is reported. Primary monocytes and the monocytic cell lines U-937 and THP-1 were infected with NTHi strain 772 and the mutant 772 Delta hpd 1 (lacking the gene for protein D). NTHi 772 adhered to and entered monocytic cells up to four-fold more efficiently compared to 772 Delta hpd 1. When an Escherichia coli transformant expressing protein D was incubated with monocytic cells, the number of intracellular bacteria increased 1.6-fold compared to protein D-deficient controls. Any correlation between internalization and phosphorylcholine expression was not detected. In conclusion, our data suggest that surface-expressed protein D promotes the adherence of NTHi to human monocytes leading to a higher number of internalized bacteria. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
non-typeable Haemophilus influenzae, internalization, glycerophosphodiester phosphodiesterase, protein D, monocytes
in
Microbial Pathogenesis
volume
31
issue
3
pages
151 - 158
publisher
Academic Press
external identifiers
  • pmid:11500100
  • scopus:0034866228
ISSN
1096-1208
DOI
10.1006/mpat.2001.0456
language
English
LU publication?
yes
id
c100d656-7c1a-4c82-8a1c-571bafa41783 (old id 1120776)
date added to LUP
2008-06-23 14:21:49
date last changed
2018-05-29 10:26:13
@article{c100d656-7c1a-4c82-8a1c-571bafa41783,
  abstract     = {Protein D, having a glycerol-3-phosphodiester phosphodiesterase activity, is found at the surface of all Haemophilus influenzae strains and is a possible virulence factor. In the present study, the involvement of protein D in the entry of NTHi into human monocytic cells is reported. Primary monocytes and the monocytic cell lines U-937 and THP-1 were infected with NTHi strain 772 and the mutant 772 Delta hpd 1 (lacking the gene for protein D). NTHi 772 adhered to and entered monocytic cells up to four-fold more efficiently compared to 772 Delta hpd 1. When an Escherichia coli transformant expressing protein D was incubated with monocytic cells, the number of intracellular bacteria increased 1.6-fold compared to protein D-deficient controls. Any correlation between internalization and phosphorylcholine expression was not detected. In conclusion, our data suggest that surface-expressed protein D promotes the adherence of NTHi to human monocytes leading to a higher number of internalized bacteria.},
  author       = {Lazou Ahrén, Irini and Janson, Håkan and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {1096-1208},
  keyword      = {non-typeable Haemophilus influenzae,internalization,glycerophosphodiester phosphodiesterase,protein D,monocytes},
  language     = {eng},
  number       = {3},
  pages        = {151--158},
  publisher    = {Academic Press},
  series       = {Microbial Pathogenesis},
  title        = {Protein D expression promotes the adherence and internalization of non-typeable Haemophilus influenzae into human monocytic cells},
  url          = {http://dx.doi.org/10.1006/mpat.2001.0456},
  volume       = {31},
  year         = {2001},
}