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RhoB controls the Rab11-mediated recycling and surface reappearance of LFA-1 in migrating T lymphocytes

Samuelsson, Malin LU ; Potrzebowska, Katarzyna LU ; Lehtonen, Janne LU ; Beech, Jason P. LU ; Skorova, Ekatarina; Uronen-Hansson, Heli LU and Svensson, Lena LU (2017) In Science Signaling 10(509).
Abstract

The regulation of cell adhesion and motility is complex and requires the intracellular trafficking of integrins to and from sites of cell adhesion, especially in fast-moving cells such as leukocytes. The Rab family of guanosine triphosphatases (GTPases) is essential for vesicle transport, and vesicles mediate intracellular integrin trafficking. We showed that RhoB regulates the vesicular transport of the integrin LFA-1 along the microtubule network in migrating T lymphocytes. Impairment in RhoB function resulted in the accumulation of both LFA-1 and the recycling endosomal marker Rab11 at the rear of migrating T lymphocytes and decreased the association between these molecules. T lymphocytes lacking functional RhoB exhibited impaired... (More)

The regulation of cell adhesion and motility is complex and requires the intracellular trafficking of integrins to and from sites of cell adhesion, especially in fast-moving cells such as leukocytes. The Rab family of guanosine triphosphatases (GTPases) is essential for vesicle transport, and vesicles mediate intracellular integrin trafficking. We showed that RhoB regulates the vesicular transport of the integrin LFA-1 along the microtubule network in migrating T lymphocytes. Impairment in RhoB function resulted in the accumulation of both LFA-1 and the recycling endosomal marker Rab11 at the rear of migrating T lymphocytes and decreased the association between these molecules. T lymphocytes lacking functional RhoB exhibited impaired recycling and subsequently decreased surface amounts of LFA-1, leading to reduced T cell adhesion and migration mediated by the cell adhesion molecule ICAM-1 (intercellular adhesion molecule-1). We propose that vesicle-associated RhoB is a regulator of the Rab11-mediated recycling of LFA-1 to the cell surface, an event that is necessary for T lymphocyte motility.

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organization
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type
Contribution to journal
publication status
published
subject
in
Science Signaling
volume
10
issue
509
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • scopus:85038418417
  • wos:000417850600001
ISSN
1945-0877
DOI
10.1126/scisignal.aai8629
language
English
LU publication?
yes
id
c121bf81-f9c5-41d8-8801-2078e870348d
date added to LUP
2018-01-03 12:40:13
date last changed
2018-01-16 13:28:51
@article{c121bf81-f9c5-41d8-8801-2078e870348d,
  abstract     = {<p>The regulation of cell adhesion and motility is complex and requires the intracellular trafficking of integrins to and from sites of cell adhesion, especially in fast-moving cells such as leukocytes. The Rab family of guanosine triphosphatases (GTPases) is essential for vesicle transport, and vesicles mediate intracellular integrin trafficking. We showed that RhoB regulates the vesicular transport of the integrin LFA-1 along the microtubule network in migrating T lymphocytes. Impairment in RhoB function resulted in the accumulation of both LFA-1 and the recycling endosomal marker Rab11 at the rear of migrating T lymphocytes and decreased the association between these molecules. T lymphocytes lacking functional RhoB exhibited impaired recycling and subsequently decreased surface amounts of LFA-1, leading to reduced T cell adhesion and migration mediated by the cell adhesion molecule ICAM-1 (intercellular adhesion molecule-1). We propose that vesicle-associated RhoB is a regulator of the Rab11-mediated recycling of LFA-1 to the cell surface, an event that is necessary for T lymphocyte motility.</p>},
  articleno    = {eaai8629},
  author       = {Samuelsson, Malin and Potrzebowska, Katarzyna and Lehtonen, Janne and Beech, Jason P. and Skorova, Ekatarina and Uronen-Hansson, Heli and Svensson, Lena},
  issn         = {1945-0877},
  language     = {eng},
  month        = {12},
  number       = {509},
  publisher    = {American Association for the Advancement of Science (AAAS)},
  series       = {Science Signaling},
  title        = {RhoB controls the Rab11-mediated recycling and surface reappearance of LFA-1 in migrating T lymphocytes},
  url          = {http://dx.doi.org/10.1126/scisignal.aai8629},
  volume       = {10},
  year         = {2017},
}