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Inhalation of hydrogenated vegetable oil combustion exhaust and genotoxicity responses in humans

Scholten, Rebecca Harnung ; Essig, Yona J ; Roursgaard, Martin ; Jensen, Annie ; Krais, Annette M LU orcid ; Gren, Louise LU ; Dierschke, Katrin LU ; Gudmundsson, Anders LU ; Wierzbicka, Aneta LU orcid and Møller, Peter (2021) In Archives of Toxicology 95(10). p.3407-3416
Abstract

Biofuels from vegetable oils or animal fats are considered to be more sustainable than petroleum-derived diesel fuel. In this study, we have assessed the effect of hydrogenated vegetable oil (HVO) exhaust on levels of DNA damage in peripheral blood mononuclear cells (PBMCs) as primary outcome, and oxidative stress and inflammation as mediators of genotoxicity. In a randomized cross-over study, healthy humans were exposed to filtered air, inorganic salt particles, exhausts from combustion of HVO in engines with aftertreatment [i.e. emission with nitrogen oxides and low amounts of particulate matter less than 2.5 µm (approximately 1 µg/m3)], or without aftertreatment (i.e. emission with nitrogen oxides and 93 ± 13 µg/m3 of PM2.5). The... (More)

Biofuels from vegetable oils or animal fats are considered to be more sustainable than petroleum-derived diesel fuel. In this study, we have assessed the effect of hydrogenated vegetable oil (HVO) exhaust on levels of DNA damage in peripheral blood mononuclear cells (PBMCs) as primary outcome, and oxidative stress and inflammation as mediators of genotoxicity. In a randomized cross-over study, healthy humans were exposed to filtered air, inorganic salt particles, exhausts from combustion of HVO in engines with aftertreatment [i.e. emission with nitrogen oxides and low amounts of particulate matter less than 2.5 µm (approximately 1 µg/m3)], or without aftertreatment (i.e. emission with nitrogen oxides and 93 ± 13 µg/m3 of PM2.5). The subjects were exposed for 3 h and blood samples were collected before, within 1 h after the exposure and 24 h after. None of the exposures caused generation of DNA strand breaks and oxidatively damaged DNA, or affected gene expression of factors related to DNA repair (Ogg1), antioxidant defense (Hmox1) or pro-inflammatory cytokines (Ccl2, Il8 and Tnfa) in PBMCs. The results from this study indicate that short-term HVO exhaust exposure is not associated with genotoxic hazard in humans.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Archives of Toxicology
volume
95
issue
10
pages
3407 - 3416
publisher
Springer
external identifiers
  • scopus:85114017373
  • pmid:34468814
ISSN
0340-5761
DOI
10.1007/s00204-021-03143-8
language
English
LU publication?
yes
id
c1efa830-ef39-4c95-82b7-2f4cac3ce7fe
date added to LUP
2021-09-10 08:57:20
date last changed
2024-06-29 17:12:23
@article{c1efa830-ef39-4c95-82b7-2f4cac3ce7fe,
  abstract     = {{<p>Biofuels from vegetable oils or animal fats are considered to be more sustainable than petroleum-derived diesel fuel. In this study, we have assessed the effect of hydrogenated vegetable oil (HVO) exhaust on levels of DNA damage in peripheral blood mononuclear cells (PBMCs) as primary outcome, and oxidative stress and inflammation as mediators of genotoxicity. In a randomized cross-over study, healthy humans were exposed to filtered air, inorganic salt particles, exhausts from combustion of HVO in engines with aftertreatment [i.e. emission with nitrogen oxides and low amounts of particulate matter less than 2.5 µm (approximately 1 µg/m3)], or without aftertreatment (i.e. emission with nitrogen oxides and 93 ± 13 µg/m3 of PM2.5). The subjects were exposed for 3 h and blood samples were collected before, within 1 h after the exposure and 24 h after. None of the exposures caused generation of DNA strand breaks and oxidatively damaged DNA, or affected gene expression of factors related to DNA repair (Ogg1), antioxidant defense (Hmox1) or pro-inflammatory cytokines (Ccl2, Il8 and Tnfa) in PBMCs. The results from this study indicate that short-term HVO exhaust exposure is not associated with genotoxic hazard in humans.</p>}},
  author       = {{Scholten, Rebecca Harnung and Essig, Yona J and Roursgaard, Martin and Jensen, Annie and Krais, Annette M and Gren, Louise and Dierschke, Katrin and Gudmundsson, Anders and Wierzbicka, Aneta and Møller, Peter}},
  issn         = {{0340-5761}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{10}},
  pages        = {{3407--3416}},
  publisher    = {{Springer}},
  series       = {{Archives of Toxicology}},
  title        = {{Inhalation of hydrogenated vegetable oil combustion exhaust and genotoxicity responses in humans}},
  url          = {{http://dx.doi.org/10.1007/s00204-021-03143-8}},
  doi          = {{10.1007/s00204-021-03143-8}},
  volume       = {{95}},
  year         = {{2021}},
}