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Acoustofluidic hematocrit determination

Petersson, Klara LU ; Jakobsson, Ola LU ; Ohlsson, Pelle LU orcid ; Augustsson, Per LU ; Scheding, Stefan LU ; Malm, Johan LU and Laurell, Thomas LU (2018) In Analytica Chimica Acta 1000. p.199-204
Abstract

Hematocrit (HCT) measurements of blood from patients, blood donors and athletes are routinely performed on a daily basis. These measurements are often performed in centralized hospital labs by whole blood analyzers, which leads to long time-to-result. On site measurements, based on centrifugation can be done, but these assays require manual handling, are slow and can just measure HCT in contrast to the central lab whole blood analyzers. In this work, we present a microfluidic based method to measure HCT in blood samples by acoustic separation of whole blood into discrete regions of plasma and red blood cells. Comparison of the areas of the red blood cell and plasma regions gives an accurate HCT value, with a linear correlation to the... (More)

Hematocrit (HCT) measurements of blood from patients, blood donors and athletes are routinely performed on a daily basis. These measurements are often performed in centralized hospital labs by whole blood analyzers, which leads to long time-to-result. On site measurements, based on centrifugation can be done, but these assays require manual handling, are slow and can just measure HCT in contrast to the central lab whole blood analyzers. In this work, we present a microfluidic based method to measure HCT in blood samples by acoustic separation of whole blood into discrete regions of plasma and red blood cells. Comparison of the areas of the red blood cell and plasma regions gives an accurate HCT value, with a linear correlation to the centrifugation-based reference method. A readout can be performed within 2 s of acoustic actuation providing a readout accuracy of approximately 3% points (pp) HCT. Additional accuracy can be achieved by extending the acoustic actuation to 20 s, yielding an error of less than 1 pp HCT. This acoustic tool is optimal for integration into a lab-on-a-chip device with in-line measurements of different clinical parameters.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acoustofluidic, Acoustophoresis, Hematocrit, Microfluidic blood analyze
in
Analytica Chimica Acta
volume
1000
pages
199 - 204
publisher
Elsevier
external identifiers
  • wos:000418832900019
  • pmid:29289309
  • scopus:85034836011
ISSN
0003-2670
DOI
10.1016/j.aca.2017.11.037
language
English
LU publication?
yes
id
c21d0175-9b1b-4cad-83d6-2c21ed780d68
date added to LUP
2017-12-06 14:24:49
date last changed
2025-01-08 02:00:42
@article{c21d0175-9b1b-4cad-83d6-2c21ed780d68,
  abstract     = {{<p>Hematocrit (HCT) measurements of blood from patients, blood donors and athletes are routinely performed on a daily basis. These measurements are often performed in centralized hospital labs by whole blood analyzers, which leads to long time-to-result. On site measurements, based on centrifugation can be done, but these assays require manual handling, are slow and can just measure HCT in contrast to the central lab whole blood analyzers. In this work, we present a microfluidic based method to measure HCT in blood samples by acoustic separation of whole blood into discrete regions of plasma and red blood cells. Comparison of the areas of the red blood cell and plasma regions gives an accurate HCT value, with a linear correlation to the centrifugation-based reference method. A readout can be performed within 2 s of acoustic actuation providing a readout accuracy of approximately 3% points (pp) HCT. Additional accuracy can be achieved by extending the acoustic actuation to 20 s, yielding an error of less than 1 pp HCT. This acoustic tool is optimal for integration into a lab-on-a-chip device with in-line measurements of different clinical parameters.</p>}},
  author       = {{Petersson, Klara and Jakobsson, Ola and Ohlsson, Pelle and Augustsson, Per and Scheding, Stefan and Malm, Johan and Laurell, Thomas}},
  issn         = {{0003-2670}},
  keywords     = {{Acoustofluidic; Acoustophoresis; Hematocrit; Microfluidic blood analyze}},
  language     = {{eng}},
  pages        = {{199--204}},
  publisher    = {{Elsevier}},
  series       = {{Analytica Chimica Acta}},
  title        = {{Acoustofluidic hematocrit determination}},
  url          = {{http://dx.doi.org/10.1016/j.aca.2017.11.037}},
  doi          = {{10.1016/j.aca.2017.11.037}},
  volume       = {{1000}},
  year         = {{2018}},
}