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Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity

Fenton, Thomas M. ; Jørgensen, Peter B. ; Niss, Kristoffer ; Rubin, Samuel J.S. ; Mörbe, Urs M. LU ; Riis, Lene B. ; Da Silva, Clément LU ; Plumb, Adam LU ; Vandamme, Julien and Jakobsen, Henrik L. , et al. (2020) In Immunity 52(3). p.557-570
Abstract

The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal... (More)

The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
colon, human intestinal regional mucosal follicles, IgA, iIeum, ILF, PP
in
Immunity
volume
52
issue
3
pages
21 pages
publisher
Cell Press
external identifiers
  • scopus:85081279962
  • pmid:32160523
ISSN
1074-7613
DOI
10.1016/j.immuni.2020.02.001
language
English
LU publication?
yes
id
c38d2820-e539-4202-913c-9aaf12cc1a52
date added to LUP
2020-03-31 15:17:19
date last changed
2024-04-17 06:36:00
@article{c38d2820-e539-4202-913c-9aaf12cc1a52,
  abstract     = {{<p>The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1<sup>+</sup> high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.</p>}},
  author       = {{Fenton, Thomas M. and Jørgensen, Peter B. and Niss, Kristoffer and Rubin, Samuel J.S. and Mörbe, Urs M. and Riis, Lene B. and Da Silva, Clément and Plumb, Adam and Vandamme, Julien and Jakobsen, Henrik L. and Brunak, Søren and Habtezion, Aida and Nielsen, Ole H. and Johansson-Lindbom, Bengt and Agace, William W.}},
  issn         = {{1074-7613}},
  keywords     = {{colon; human intestinal regional mucosal follicles; IgA; iIeum; ILF; PP}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{3}},
  pages        = {{557--570}},
  publisher    = {{Cell Press}},
  series       = {{Immunity}},
  title        = {{Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity}},
  url          = {{http://dx.doi.org/10.1016/j.immuni.2020.02.001}},
  doi          = {{10.1016/j.immuni.2020.02.001}},
  volume       = {{52}},
  year         = {{2020}},
}