Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity
(2020) In Immunity 52(3). p.557-570- Abstract
The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal... (More)
The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.
(Less)
- author
- organization
- publishing date
- 2020-03-17
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- colon, human intestinal regional mucosal follicles, IgA, iIeum, ILF, PP
- in
- Immunity
- volume
- 52
- issue
- 3
- pages
- 21 pages
- publisher
- Cell Press
- external identifiers
-
- scopus:85081279962
- pmid:32160523
- ISSN
- 1074-7613
- DOI
- 10.1016/j.immuni.2020.02.001
- language
- English
- LU publication?
- yes
- id
- c38d2820-e539-4202-913c-9aaf12cc1a52
- date added to LUP
- 2020-03-31 15:17:19
- date last changed
- 2024-04-17 06:36:00
@article{c38d2820-e539-4202-913c-9aaf12cc1a52, abstract = {{<p>The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1<sup>+</sup> high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.</p>}}, author = {{Fenton, Thomas M. and Jørgensen, Peter B. and Niss, Kristoffer and Rubin, Samuel J.S. and Mörbe, Urs M. and Riis, Lene B. and Da Silva, Clément and Plumb, Adam and Vandamme, Julien and Jakobsen, Henrik L. and Brunak, Søren and Habtezion, Aida and Nielsen, Ole H. and Johansson-Lindbom, Bengt and Agace, William W.}}, issn = {{1074-7613}}, keywords = {{colon; human intestinal regional mucosal follicles; IgA; iIeum; ILF; PP}}, language = {{eng}}, month = {{03}}, number = {{3}}, pages = {{557--570}}, publisher = {{Cell Press}}, series = {{Immunity}}, title = {{Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity}}, url = {{http://dx.doi.org/10.1016/j.immuni.2020.02.001}}, doi = {{10.1016/j.immuni.2020.02.001}}, volume = {{52}}, year = {{2020}}, }