Diet-Induced Obesity Modulates Close-Packing of Triacylglycerols in Lipid Droplets of Adipose Tissue
(2024) In Journal of the American Chemical Society 146(50). p.34796-34810- Abstract
Adipose-derived lipid droplets (LDs) are rich in triacylglycerols (TAGs), which regulate essential cellular processes, such as energy storage. Although TAG accumulation and LD expansion in adipocytes occur during obesity, how LDs dynamically package TAGs in response to excessive nutrients remains elusive. Here, we found that LD lipidomes display a remarkable increase in TAG acyl chain saturation under calorie-dense diets, turning them conducive to close-packing. Using high-resolution X-ray diffraction, solid-state NMR, and imaging, we show that beyond size expansion LDs from mice under varied obesogenic diets govern fat accumulation by packing TAGs in different crystalline polymorphs. Consistently, LDs and tissue stiffen for... (More)
Adipose-derived lipid droplets (LDs) are rich in triacylglycerols (TAGs), which regulate essential cellular processes, such as energy storage. Although TAG accumulation and LD expansion in adipocytes occur during obesity, how LDs dynamically package TAGs in response to excessive nutrients remains elusive. Here, we found that LD lipidomes display a remarkable increase in TAG acyl chain saturation under calorie-dense diets, turning them conducive to close-packing. Using high-resolution X-ray diffraction, solid-state NMR, and imaging, we show that beyond size expansion LDs from mice under varied obesogenic diets govern fat accumulation by packing TAGs in different crystalline polymorphs. Consistently, LDs and tissue stiffen for high-calorie-fed mice with more than a 2-fold increase in elastic moduli compared to normal diet. Our data suggest that in addition to expanding, adipocyte LDs undergo structural remodeling by close-packing rigid and highly saturated TAGs in response to caloric overload, as opposed to liquid TAGs in a low-calorie diet. This work provides insights into how lipid packing within LDs can allow for the rapid and optimal expansion of fat during the initial stages of obesity.
(Less)
- author
- organization
- publishing date
- 2024-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cryo-EM, fat packing, lipid droplets, micropipette aspiration, obesity, solid-state NMR, X-ray diffraction
- in
- Journal of the American Chemical Society
- volume
- 146
- issue
- 50
- pages
- 15 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85211354365
- pmid:39644234
- ISSN
- 0002-7863
- DOI
- 10.1021/jacs.4c13420
- language
- English
- LU publication?
- yes
- id
- c4ab20cc-9909-40af-b0e0-f139313d6e40
- date added to LUP
- 2025-01-16 15:13:51
- date last changed
- 2025-07-18 06:24:17
@article{c4ab20cc-9909-40af-b0e0-f139313d6e40,
abstract = {{<p>Adipose-derived lipid droplets (LDs) are rich in triacylglycerols (TAGs), which regulate essential cellular processes, such as energy storage. Although TAG accumulation and LD expansion in adipocytes occur during obesity, how LDs dynamically package TAGs in response to excessive nutrients remains elusive. Here, we found that LD lipidomes display a remarkable increase in TAG acyl chain saturation under calorie-dense diets, turning them conducive to close-packing. Using high-resolution X-ray diffraction, solid-state NMR, and imaging, we show that beyond size expansion LDs from mice under varied obesogenic diets govern fat accumulation by packing TAGs in different crystalline polymorphs. Consistently, LDs and tissue stiffen for high-calorie-fed mice with more than a 2-fold increase in elastic moduli compared to normal diet. Our data suggest that in addition to expanding, adipocyte LDs undergo structural remodeling by close-packing rigid and highly saturated TAGs in response to caloric overload, as opposed to liquid TAGs in a low-calorie diet. This work provides insights into how lipid packing within LDs can allow for the rapid and optimal expansion of fat during the initial stages of obesity.</p>}},
author = {{Ko, Kyungwon and Bandara, Sarith R. and Zhou, Weinan and Svenningsson, Leo and Porras-Gómez, Marilyn and Kambar, Nurila and Dreher-Threlkeld, Julia and Topgaard, Daniel and Hernández-Saavedra, Diego and Anakk, Sayeepriyadarshini and Leal, Cecília}},
issn = {{0002-7863}},
keywords = {{cryo-EM; fat packing; lipid droplets; micropipette aspiration; obesity; solid-state NMR; X-ray diffraction}},
language = {{eng}},
number = {{50}},
pages = {{34796--34810}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of the American Chemical Society}},
title = {{Diet-Induced Obesity Modulates Close-Packing of Triacylglycerols in Lipid Droplets of Adipose Tissue}},
url = {{http://dx.doi.org/10.1021/jacs.4c13420}},
doi = {{10.1021/jacs.4c13420}},
volume = {{146}},
year = {{2024}},
}