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Improvement of thermotolerance in Lachancea thermotolerans using a bacterial selection pressure

Zhou, Nerve LU ; Ishchuk, Olena P. LU ; Knecht, Wolfgang LU ; Compagno, Concetta and Piškur, Jure LU (2019) In Journal of Industrial Microbiology and Biotechnology 46(2). p.133-145
Abstract

The use of thermotolerant yeast strains is an important attribute for a cost-effective high temperature biofermentation processes. However, the availability of thermotolerant yeast strains remains a major challenge. Isolation of temperature resistant strains from extreme environments or the improvements of current strains are two major strategies known to date. We hypothesised that bacteria are potential “hurdles” in the life cycle of yeasts, which could influence the evolution of extreme phenotypes, such as thermotolerance. We subjected a wild-type yeast, Lachancea thermotolerans to six species of bacteria sequentially for several generations. After coevolution, we observed that three replicate lines of yeasts grown in the presence of... (More)

The use of thermotolerant yeast strains is an important attribute for a cost-effective high temperature biofermentation processes. However, the availability of thermotolerant yeast strains remains a major challenge. Isolation of temperature resistant strains from extreme environments or the improvements of current strains are two major strategies known to date. We hypothesised that bacteria are potential “hurdles” in the life cycle of yeasts, which could influence the evolution of extreme phenotypes, such as thermotolerance. We subjected a wild-type yeast, Lachancea thermotolerans to six species of bacteria sequentially for several generations. After coevolution, we observed that three replicate lines of yeasts grown in the presence of bacteria grew up to 37 °C whereas the controls run in parallel without bacteria could only grow poorly at 35 °C retaining the ancestral mesophilic trait. In addition to improvement of thermotolerance, our results show that the fermentative ability was also elevated, making the strains more ideal for the alcoholic fermentation process because the overall productivity and ethanol titers per unit volume of substrate consumed during the fermentation process was increased. Our unique method is attractive for the development of thermotolerant strains or to augment the available strain development approaches for high temperature industrial biofermentation.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alcoholic fermentation, Experimental evolution, Thermotolerance, Yeast-bacteria coevolution
in
Journal of Industrial Microbiology and Biotechnology
volume
46
issue
2
pages
133 - 145
publisher
Springer
external identifiers
  • scopus:85057534628
  • pmid:30488364
ISSN
1367-5435
DOI
10.1007/s10295-018-2107-4
language
English
LU publication?
yes
id
c8ecff35-4777-4368-963a-4f0d8f197d74
date added to LUP
2018-12-20 14:02:48
date last changed
2020-01-13 01:18:24
@article{c8ecff35-4777-4368-963a-4f0d8f197d74,
  abstract     = {<p>The use of thermotolerant yeast strains is an important attribute for a cost-effective high temperature biofermentation processes. However, the availability of thermotolerant yeast strains remains a major challenge. Isolation of temperature resistant strains from extreme environments or the improvements of current strains are two major strategies known to date. We hypothesised that bacteria are potential “hurdles” in the life cycle of yeasts, which could influence the evolution of extreme phenotypes, such as thermotolerance. We subjected a wild-type yeast, Lachancea thermotolerans to six species of bacteria sequentially for several generations. After coevolution, we observed that three replicate lines of yeasts grown in the presence of bacteria grew up to 37 °C whereas the controls run in parallel without bacteria could only grow poorly at 35 °C retaining the ancestral mesophilic trait. In addition to improvement of thermotolerance, our results show that the fermentative ability was also elevated, making the strains more ideal for the alcoholic fermentation process because the overall productivity and ethanol titers per unit volume of substrate consumed during the fermentation process was increased. Our unique method is attractive for the development of thermotolerant strains or to augment the available strain development approaches for high temperature industrial biofermentation.</p>},
  author       = {Zhou, Nerve and Ishchuk, Olena P. and Knecht, Wolfgang and Compagno, Concetta and Piškur, Jure},
  issn         = {1367-5435},
  language     = {eng},
  number       = {2},
  pages        = {133--145},
  publisher    = {Springer},
  series       = {Journal of Industrial Microbiology and Biotechnology},
  title        = {Improvement of thermotolerance in Lachancea thermotolerans using a bacterial selection pressure},
  url          = {http://dx.doi.org/10.1007/s10295-018-2107-4},
  doi          = {10.1007/s10295-018-2107-4},
  volume       = {46},
  year         = {2019},
}