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Immunohistochemical profiles in primary lung cancers and epithelial pulmonary metastases

Vidarsdottir, Halla LU ; Tran, Lena; Nodin, Björn LU ; Jirström, Karin LU ; Planck, Maria LU ; Jönsson, Per LU ; Mattsson, Johanna S.M.; Botling, Johan; Micke, Patrick and Brunnström, Hans LU (2019) In Human Pathology 84. p.221-230
Abstract

Correct diagnosis of pulmonary tumors is essential for treatment decision and often relies on immunohistochemical markers. We stained tissue microarrays from resected primary lung cancer (n = 665) and pulmonary metastases (n = 425) for CK7, CK20, CDX2, CK5, p40, p63, TTF-1, napsin A, GATA3, and PAX8 to systematically assess the diagnostic value of these markers. Primary lung adenocarcinomas expressed TTF-1 in 90% and napsin A in 84% of the cases, whereas 10% were positive for p63, 7% for CDX2, 2% for CK20, and 2% for GATA3. Only 68% of the lung adenocarcinomas were positive for CK7, TTF-1, and napsin A and negative for all other markers. Primary lung squamous cell carcinomas expressed CK5, p40, and p63 in 94%-97% of cases, whereas 44%... (More)

Correct diagnosis of pulmonary tumors is essential for treatment decision and often relies on immunohistochemical markers. We stained tissue microarrays from resected primary lung cancer (n = 665) and pulmonary metastases (n = 425) for CK7, CK20, CDX2, CK5, p40, p63, TTF-1, napsin A, GATA3, and PAX8 to systematically assess the diagnostic value of these markers. Primary lung adenocarcinomas expressed TTF-1 in 90% and napsin A in 84% of the cases, whereas 10% were positive for p63, 7% for CDX2, 2% for CK20, and 2% for GATA3. Only 68% of the lung adenocarcinomas were positive for CK7, TTF-1, and napsin A and negative for all other markers. Primary lung squamous cell carcinomas expressed CK5, p40, and p63 in 94%-97% of cases, whereas 44% were positive for CK7, 20% for GATA3, 7% for CDX2, and 3% for TTF-1. Rare cases expressed PAX8, CK20, or napsin A. Pulmonary metastases of colorectal cancer were positive for CK20 in 83% and CDX2 in 99% of the cases. Rare cases expressed CK7, p63, or PAX8, whereas 4% expressed TTF-1. Pulmonary metastases of renal cell carcinomas were positive for PAX8 in 74%, napsin A in 7%, and CK7 in 7% of the cases. Pulmonary metastases of breast cancer were positive for GATA3 in 93% and CK7 in 78% of the cases, whereas 15% expressed CK5. Information on expression and patterns of immunohistochemical markers facilitates histopathological diagnostics. Evidently, unusual immune profiles occur and may lead to incorrect diagnosis.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CDX2, Cytokeratin, GATA3, Napsin A, Tissue microarray, TTF-1
in
Human Pathology
volume
84
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85060112849
ISSN
0046-8177
DOI
10.1016/j.humpath.2018.10.009
language
English
LU publication?
yes
id
c95e777f-4e87-48e6-ac8a-4b6d8a7922ca
date added to LUP
2019-01-28 12:50:01
date last changed
2019-02-20 11:44:45
@article{c95e777f-4e87-48e6-ac8a-4b6d8a7922ca,
  abstract     = {<p>Correct diagnosis of pulmonary tumors is essential for treatment decision and often relies on immunohistochemical markers. We stained tissue microarrays from resected primary lung cancer (n = 665) and pulmonary metastases (n = 425) for CK7, CK20, CDX2, CK5, p40, p63, TTF-1, napsin A, GATA3, and PAX8 to systematically assess the diagnostic value of these markers. Primary lung adenocarcinomas expressed TTF-1 in 90% and napsin A in 84% of the cases, whereas 10% were positive for p63, 7% for CDX2, 2% for CK20, and 2% for GATA3. Only 68% of the lung adenocarcinomas were positive for CK7, TTF-1, and napsin A and negative for all other markers. Primary lung squamous cell carcinomas expressed CK5, p40, and p63 in 94%-97% of cases, whereas 44% were positive for CK7, 20% for GATA3, 7% for CDX2, and 3% for TTF-1. Rare cases expressed PAX8, CK20, or napsin A. Pulmonary metastases of colorectal cancer were positive for CK20 in 83% and CDX2 in 99% of the cases. Rare cases expressed CK7, p63, or PAX8, whereas 4% expressed TTF-1. Pulmonary metastases of renal cell carcinomas were positive for PAX8 in 74%, napsin A in 7%, and CK7 in 7% of the cases. Pulmonary metastases of breast cancer were positive for GATA3 in 93% and CK7 in 78% of the cases, whereas 15% expressed CK5. Information on expression and patterns of immunohistochemical markers facilitates histopathological diagnostics. Evidently, unusual immune profiles occur and may lead to incorrect diagnosis.</p>},
  author       = {Vidarsdottir, Halla and Tran, Lena and Nodin, Björn and Jirström, Karin and Planck, Maria and Jönsson, Per and Mattsson, Johanna S.M. and Botling, Johan and Micke, Patrick and Brunnström, Hans},
  issn         = {0046-8177},
  keyword      = {CDX2,Cytokeratin,GATA3,Napsin A,Tissue microarray,TTF-1},
  language     = {eng},
  pages        = {221--230},
  publisher    = {Elsevier},
  series       = {Human Pathology},
  title        = {Immunohistochemical profiles in primary lung cancers and epithelial pulmonary metastases},
  url          = {http://dx.doi.org/10.1016/j.humpath.2018.10.009},
  volume       = {84},
  year         = {2019},
}