Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells
(2018) In Scientific Reports 8(1).- Abstract
Chronic obstructive pulmonary disease (COPD) is a serious global health problem characterized by chronic airway inflammation, progressive airflow limitation and destruction of lung parenchyma. Remodeling of the bronchial airways in COPD includes changes in both the bronchial epithelium and the subepithelial extracellular matrix (ECM). To explore the impact of an aberrant ECM on epithelial cell phenotype in COPD we developed a new ex vivo model, in which normal human bronchial epithelial (NHBE) cells repopulate and differentiate on decellularized human bronchial scaffolds derived from COPD patients and healthy individuals. By using transcriptomics, we show that bronchial ECM from COPD patients induces differential gene expression in... (More)
Chronic obstructive pulmonary disease (COPD) is a serious global health problem characterized by chronic airway inflammation, progressive airflow limitation and destruction of lung parenchyma. Remodeling of the bronchial airways in COPD includes changes in both the bronchial epithelium and the subepithelial extracellular matrix (ECM). To explore the impact of an aberrant ECM on epithelial cell phenotype in COPD we developed a new ex vivo model, in which normal human bronchial epithelial (NHBE) cells repopulate and differentiate on decellularized human bronchial scaffolds derived from COPD patients and healthy individuals. By using transcriptomics, we show that bronchial ECM from COPD patients induces differential gene expression in primary NHBE cells when compared to normal bronchial ECM. The gene expression profile indicated altered activity of upstream mediators associated with COPD pathophysiology, including hepatocyte growth factor, transforming growth factor beta 1 and platelet-derived growth factor B, which suggests that COPD-related changes in the bronchial ECM contribute to the defective regenerative ability in the airways of COPD patients.
(Less)
- author
- Hedström, Ulf LU ; Hallgren, Oskar LU ; Öberg, Lisa ; Demicco, Amy ; Vaarala, Outi ; Westergren-Thorsson, Gunilla LU and Zhou, Xiaohong
- organization
- publishing date
- 2018-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 8
- issue
- 1
- article number
- 3502
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:29472603
- scopus:85042536613
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-018-21727-w
- language
- English
- LU publication?
- yes
- id
- cc7cb18d-8aea-4959-b89d-1860af9feba2
- date added to LUP
- 2018-03-08 08:14:07
- date last changed
- 2024-09-16 18:20:43
@article{cc7cb18d-8aea-4959-b89d-1860af9feba2, abstract = {{<p>Chronic obstructive pulmonary disease (COPD) is a serious global health problem characterized by chronic airway inflammation, progressive airflow limitation and destruction of lung parenchyma. Remodeling of the bronchial airways in COPD includes changes in both the bronchial epithelium and the subepithelial extracellular matrix (ECM). To explore the impact of an aberrant ECM on epithelial cell phenotype in COPD we developed a new ex vivo model, in which normal human bronchial epithelial (NHBE) cells repopulate and differentiate on decellularized human bronchial scaffolds derived from COPD patients and healthy individuals. By using transcriptomics, we show that bronchial ECM from COPD patients induces differential gene expression in primary NHBE cells when compared to normal bronchial ECM. The gene expression profile indicated altered activity of upstream mediators associated with COPD pathophysiology, including hepatocyte growth factor, transforming growth factor beta 1 and platelet-derived growth factor B, which suggests that COPD-related changes in the bronchial ECM contribute to the defective regenerative ability in the airways of COPD patients.</p>}}, author = {{Hedström, Ulf and Hallgren, Oskar and Öberg, Lisa and Demicco, Amy and Vaarala, Outi and Westergren-Thorsson, Gunilla and Zhou, Xiaohong}}, issn = {{2045-2322}}, language = {{eng}}, month = {{12}}, number = {{1}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{Bronchial extracellular matrix from COPD patients induces altered gene expression in repopulated primary human bronchial epithelial cells}}, url = {{http://dx.doi.org/10.1038/s41598-018-21727-w}}, doi = {{10.1038/s41598-018-21727-w}}, volume = {{8}}, year = {{2018}}, }