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The Influence of pH on the Lipase Digestion of Nanosized Triolein, Diolein and Monoolein films

Humphreys, Ben A. LU ; Campos-Terán, José LU ; Arnold, Thomas ; Baunsgaard, Lone ; Vind, Jesper ; Dicko, Cedric LU orcid and Nylander, Tommy LU (2022) In Frontiers in Soft Matter 2.
Abstract
Herein we studied the processes at the liquid aqueous interface at pH 7 and 8.5 during Thermomyces lanuginosus lipase-catalyzed hydrolysis of nanosized tri-, di- and mono-olein films deposited on a planar substrate. By employing a combination of ellipsometry, QCM-D and ATR-FTIR, we were able to reveal the physical properties of the thin films at high time resolution throughout the initial hydration and subsequent digestion, as well as the main chemical species present before and after lipolysis. The ATR-FTIR results showed that the degree of digestion and protonated state of the oleic acid produced in the reaction are highly dependent on the pH of the aqueous solvent. Furthermore, the ellipsometry and QCM-D results reveal that the duration... (More)
Herein we studied the processes at the liquid aqueous interface at pH 7 and 8.5 during Thermomyces lanuginosus lipase-catalyzed hydrolysis of nanosized tri-, di- and mono-olein films deposited on a planar substrate. By employing a combination of ellipsometry, QCM-D and ATR-FTIR, we were able to reveal the physical properties of the thin films at high time resolution throughout the initial hydration and subsequent digestion, as well as the main chemical species present before and after lipolysis. The ATR-FTIR results showed that the degree of digestion and protonated state of the oleic acid produced in the reaction are highly dependent on the pH of the aqueous solvent. Furthermore, the ellipsometry and QCM-D results reveal that the duration of the lag phase observed before lipolysis was detected and the magnitude and type of changes to the physical properties of the thin films throughout digestion was influenced by whether the initial substrate consisted of tri-, di- or mono-olein. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Frontiers in Soft Matter
volume
2
article number
929104
pages
13 pages
publisher
Frontiers Media S. A.
ISSN
2813-0499
DOI
10.3389/frsfm.2022.929104
language
English
LU publication?
yes
id
cc80a5a1-175e-4b53-a92f-2a088cbc0c52
date added to LUP
2022-07-28 12:21:32
date last changed
2022-08-02 09:57:22
@article{cc80a5a1-175e-4b53-a92f-2a088cbc0c52,
  abstract     = {{Herein we studied the processes at the liquid aqueous interface at pH 7 and 8.5 during Thermomyces lanuginosus lipase-catalyzed hydrolysis of nanosized tri-, di- and mono-olein films deposited on a planar substrate. By employing a combination of ellipsometry, QCM-D and ATR-FTIR, we were able to reveal the physical properties of the thin films at high time resolution throughout the initial hydration and subsequent digestion, as well as the main chemical species present before and after lipolysis. The ATR-FTIR results showed that the degree of digestion and protonated state of the oleic acid produced in the reaction are highly dependent on the pH of the aqueous solvent. Furthermore, the ellipsometry and QCM-D results reveal that the duration of the lag phase observed before lipolysis was detected and the magnitude and type of changes to the physical properties of the thin films throughout digestion was influenced by whether the initial substrate consisted of tri-, di- or mono-olein.}},
  author       = {{Humphreys, Ben A. and Campos-Terán, José and Arnold, Thomas and Baunsgaard, Lone and Vind, Jesper and Dicko, Cedric and Nylander, Tommy}},
  issn         = {{2813-0499}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Soft Matter}},
  title        = {{The Influence of pH on the Lipase Digestion of Nanosized Triolein, Diolein and Monoolein films}},
  url          = {{http://dx.doi.org/10.3389/frsfm.2022.929104}},
  doi          = {{10.3389/frsfm.2022.929104}},
  volume       = {{2}},
  year         = {{2022}},
}