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Can sensory and motor collateral sprouting be induced from intact peripheral nerve by end-to-side anastomosis?

Lundborg, G LU ; Zhao, Q; Kanje, M LU ; Danielsen, N LU and Kerns, J M (1994) In Journal of Hand Surgery: European Volume 19(3). p.82-277
Abstract

The possibility that collateral sprouting could occur from intact axons in an undamaged sciatic nerve was studied in the rat by suturing either a 7-day predegenerated or a fresh nerve segment in an end-to-side fashion to the sciatic nerve proper. Following a 14- or 35-day recovery period, the pinch reflex test was performed on the transplanted segment to demonstrate the presence of sensory axons. The majority of cases, using a predegenerated nerve segment but not a fresh segment, responded positively. Neurofilament staining and histological examination confirmed the presence of axons in the attached nerve segment. In another series of experiments, the proximal peroneal fascicle was ligated and cut. Following a 7-day predegeneration... (More)

The possibility that collateral sprouting could occur from intact axons in an undamaged sciatic nerve was studied in the rat by suturing either a 7-day predegenerated or a fresh nerve segment in an end-to-side fashion to the sciatic nerve proper. Following a 14- or 35-day recovery period, the pinch reflex test was performed on the transplanted segment to demonstrate the presence of sensory axons. The majority of cases, using a predegenerated nerve segment but not a fresh segment, responded positively. Neurofilament staining and histological examination confirmed the presence of axons in the attached nerve segment. In another series of experiments, the proximal peroneal fascicle was ligated and cut. Following a 7-day predegeneration period the distal stump was sutured end-to-side to the ipsilateral tibial fascicle. After 90 days, stimulation of the tibial nerve proximal to the attached site induced substantial contraction in both the native gastrocnemius muscle and the foreign tibialis anterior muscle. These findings suggest that collateral sprouting may occur from intact axons, perhaps induced by factors emanating from the attached nerve segment, and subsequently make functional peripheral connections.

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keywords
Anastomosis, Surgical, Animals, Axons, Female, Isometric Contraction, Motor Neurons, Muscle Contraction, Muscles, Nerve Degeneration, Nerve Regeneration, Neurofilament Proteins, Neurons, Afferent, Peroneal Nerve, Rats, Rats, Wistar, Sciatic Nerve, Tibial Nerve, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
in
Journal of Hand Surgery: European Volume
volume
19
issue
3
pages
82 - 277
publisher
SAGE Publications Inc.
external identifiers
  • scopus:0028275975
ISSN
0266-7681
DOI
10.1016/0266-7681(94)90069-8
language
English
LU publication?
yes
id
ccdf343f-3d10-4dd1-b76a-2fd2b4b7c92d
date added to LUP
2017-10-13 13:57:56
date last changed
2017-11-14 16:45:12
@article{ccdf343f-3d10-4dd1-b76a-2fd2b4b7c92d,
  abstract     = {<p>The possibility that collateral sprouting could occur from intact axons in an undamaged sciatic nerve was studied in the rat by suturing either a 7-day predegenerated or a fresh nerve segment in an end-to-side fashion to the sciatic nerve proper. Following a 14- or 35-day recovery period, the pinch reflex test was performed on the transplanted segment to demonstrate the presence of sensory axons. The majority of cases, using a predegenerated nerve segment but not a fresh segment, responded positively. Neurofilament staining and histological examination confirmed the presence of axons in the attached nerve segment. In another series of experiments, the proximal peroneal fascicle was ligated and cut. Following a 7-day predegeneration period the distal stump was sutured end-to-side to the ipsilateral tibial fascicle. After 90 days, stimulation of the tibial nerve proximal to the attached site induced substantial contraction in both the native gastrocnemius muscle and the foreign tibialis anterior muscle. These findings suggest that collateral sprouting may occur from intact axons, perhaps induced by factors emanating from the attached nerve segment, and subsequently make functional peripheral connections.</p>},
  author       = {Lundborg, G and Zhao, Q and Kanje, M and Danielsen, N and Kerns, J M},
  issn         = {0266-7681},
  keyword      = {Anastomosis, Surgical,Animals,Axons,Female,Isometric Contraction,Motor Neurons,Muscle Contraction,Muscles,Nerve Degeneration,Nerve Regeneration,Neurofilament Proteins,Neurons, Afferent,Peroneal Nerve,Rats,Rats, Wistar,Sciatic Nerve,Tibial Nerve,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, P.H.S.},
  language     = {eng},
  number       = {3},
  pages        = {82--277},
  publisher    = {SAGE Publications Inc.},
  series       = {Journal of Hand Surgery: European Volume},
  title        = {Can sensory and motor collateral sprouting be induced from intact peripheral nerve by end-to-side anastomosis?},
  url          = {http://dx.doi.org/10.1016/0266-7681(94)90069-8},
  volume       = {19},
  year         = {1994},
}