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Airway contraction and cytokine release in isolated rat lungs induced by wear particles from the road and tire interface and road vehicle brakes

Nosratabadi, Ali Reza ; Gustafsson, Mats ; Lovén, Karin LU ; Ljunggren, Stefan A. ; Olofsson, Ulf ; Abbasi, Saeed ; Blomqvist, Göran ; Karlsson, Helen ; Ljungman, Anders G. and Cassee, Flemming R. , et al. (2023) In Inhalation Toxicology 35(13-14). p.309-323
Abstract

The dominant road traffic particle sources are wear particles from the road and tire interface, and from vehicle brake pads. The aim of this work was to investigate the effect of road and brake wear particles on pulmonary function and biomarkers in isolated perfused rat lungs. Particles were sampled from the studded tire wear of three road pavements containing different rock materials in a road simulator; and from the wear of two brake pad materials using a pin-on-disk machine. Isolated rat lungs inhaled the coarse and fine fractions of the sampled particles resulting in an estimated total particle lung dose of 50 μg. The tidal volume (TV) was measured during the particle exposure and the following 50 min. Perfusate and BALF were... (More)

The dominant road traffic particle sources are wear particles from the road and tire interface, and from vehicle brake pads. The aim of this work was to investigate the effect of road and brake wear particles on pulmonary function and biomarkers in isolated perfused rat lungs. Particles were sampled from the studded tire wear of three road pavements containing different rock materials in a road simulator; and from the wear of two brake pad materials using a pin-on-disk machine. Isolated rat lungs inhaled the coarse and fine fractions of the sampled particles resulting in an estimated total particle lung dose of 50 μg. The tidal volume (TV) was measured during the particle exposure and the following 50 min. Perfusate and BALF were analyzed for the cytokines TNF, CXCL1 and CCL3. The TV of lungs exposed to rock materials was significantly reduced after 25 min of exposure compared to the controls, for quartzite already after 4 min. The particles of the heavy-duty brake pads had no effect on the TV. Brake particles resulted in a significant elevation of CXCL1 in the perfusate. Brake particles showed significant elevations of all three measured cytokines, and quartzite showed a significant elevation of TNF in BALF. The study shows that the toxic effect on lungs exposed to airborne particles can be investigated using measurements of tidal volume. Furthermore, the study shows that the choice of rock material in road pavements has the potential to affect the toxicity of road wear PM10.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biomarkers, brake wear particles, isolated perfused lung, Pulmonary disorders, road wear particles, tidal volume
in
Inhalation Toxicology
volume
35
issue
13-14
pages
15 pages
publisher
Taylor & Francis
external identifiers
  • pmid:38054445
  • scopus:85179981752
ISSN
0895-8378
DOI
10.1080/08958378.2023.2289018
language
English
LU publication?
yes
id
ce583515-7a51-4e85-8bed-540026a690de
date added to LUP
2024-01-10 11:10:14
date last changed
2024-04-25 07:07:06
@article{ce583515-7a51-4e85-8bed-540026a690de,
  abstract     = {{<p>The dominant road traffic particle sources are wear particles from the road and tire interface, and from vehicle brake pads. The aim of this work was to investigate the effect of road and brake wear particles on pulmonary function and biomarkers in isolated perfused rat lungs. Particles were sampled from the studded tire wear of three road pavements containing different rock materials in a road simulator; and from the wear of two brake pad materials using a pin-on-disk machine. Isolated rat lungs inhaled the coarse and fine fractions of the sampled particles resulting in an estimated total particle lung dose of 50 μg. The tidal volume (TV) was measured during the particle exposure and the following 50 min. Perfusate and BALF were analyzed for the cytokines TNF, CXCL1 and CCL3. The TV of lungs exposed to rock materials was significantly reduced after 25 min of exposure compared to the controls, for quartzite already after 4 min. The particles of the heavy-duty brake pads had no effect on the TV. Brake particles resulted in a significant elevation of CXCL1 in the perfusate. Brake particles showed significant elevations of all three measured cytokines, and quartzite showed a significant elevation of TNF in BALF. The study shows that the toxic effect on lungs exposed to airborne particles can be investigated using measurements of tidal volume. Furthermore, the study shows that the choice of rock material in road pavements has the potential to affect the toxicity of road wear PM10.</p>}},
  author       = {{Nosratabadi, Ali Reza and Gustafsson, Mats and Lovén, Karin and Ljunggren, Stefan A. and Olofsson, Ulf and Abbasi, Saeed and Blomqvist, Göran and Karlsson, Helen and Ljungman, Anders G. and Cassee, Flemming R. and Gerlofs-Nijland, Miriam E. and Gudmundsson, Anders}},
  issn         = {{0895-8378}},
  keywords     = {{biomarkers; brake wear particles; isolated perfused lung; Pulmonary disorders; road wear particles; tidal volume}},
  language     = {{eng}},
  number       = {{13-14}},
  pages        = {{309--323}},
  publisher    = {{Taylor & Francis}},
  series       = {{Inhalation Toxicology}},
  title        = {{Airway contraction and cytokine release in isolated rat lungs induced by wear particles from the road and tire interface and road vehicle brakes}},
  url          = {{http://dx.doi.org/10.1080/08958378.2023.2289018}},
  doi          = {{10.1080/08958378.2023.2289018}},
  volume       = {{35}},
  year         = {{2023}},
}